ID   ALF1_OCEIH              Reviewed;         294 AA.
AC   Q8ELI2;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Fructose-bisphosphate aldolase class 1 {ECO:0000255|HAMAP-Rule:MF_00729};
DE            EC=4.1.2.13 {ECO:0000255|HAMAP-Rule:MF_00729};
DE   AltName: Full=Fructose-bisphosphate aldolase class I;
DE            Short=FBP aldolase {ECO:0000255|HAMAP-Rule:MF_00729};
GN   Name=fda {ECO:0000255|HAMAP-Rule:MF_00729}; OrderedLocusNames=OB3245;
OS   Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS   3954 / HTE831).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX   NCBI_TaxID=221109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX   PubMed=12235376; DOI=10.1093/nar/gkf526;
RA   Takami H., Takaki Y., Uchiyama I.;
RT   "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT   and its unexpected adaptive capabilities to extreme environments.";
RL   Nucleic Acids Res. 30:3927-3935(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-
CC         phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729,
CC         ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00729};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 4/4.
CC       {ECO:0000255|HAMAP-Rule:MF_00729}.
CC   -!- SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_00729}.
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DR   EMBL; BA000028; BAC15201.1; -; Genomic_DNA.
DR   RefSeq; WP_011067641.1; NC_004193.1.
DR   AlphaFoldDB; Q8ELI2; -.
DR   SMR; Q8ELI2; -.
DR   STRING; 221109.22778934; -.
DR   EnsemblBacteria; BAC15201; BAC15201; BAC15201.
DR   KEGG; oih:OB3245; -.
DR   eggNOG; COG3588; Bacteria.
DR   HOGENOM; CLU_081560_0_0_9; -.
DR   OMA; GVFGTKM; -.
DR   OrthoDB; 945470at2; -.
DR   PhylomeDB; Q8ELI2; -.
DR   UniPathway; UPA00109; UER00183.
DR   Proteomes; UP000000822; Chromosome.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00949; FBP_aldolase_I_bact; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00729; FBP_aldolase_1; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000741; FBA_I.
DR   InterPro; IPR023014; FBA_I_Gram+-type.
DR   PANTHER; PTHR11627; PTHR11627; 1.
DR   Pfam; PF00274; Glycolytic; 1.
PE   3: Inferred from homology;
KW   Glycolysis; Lyase; Reference proteome; Schiff base.
FT   CHAIN           1..294
FT                   /note="Fructose-bisphosphate aldolase class 1"
FT                   /id="PRO_0000216901"
FT   ACT_SITE        176
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00729"
FT   ACT_SITE        213
FT                   /note="Schiff-base intermediate with dihydroxyacetone-P"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00729"
SQ   SEQUENCE   294 AA;  32819 MW;  76B018EE93174698 CRC64;
     MNQKQLEQMK TSKGFIAALD QSGGSTPKAL AAYGIPEDSY NNEDEMFDLV HEMRTRIITS
     KAFDSDSIIG AILFEQTMDR EIEGMYTGDY LAEKKGIVPF LKVDKGLAEE TNGVQLMKPI
     DNLDETLRRA NERNIFGTKM RSVIKQANPK AIKEVVDQQF DIGKKIIAAG LVPIIEPEVD
     IHSPEKEKCE DLLKAEIINH LNQLSEDENV MLKLTIPTKK NLYKELIDHP RVVRVVALSG
     GYSTDVANEK LKENNGLIAS FSRALSQDLN ADQSDEDFNL ALEKAVKSIY DASV