ALF1_SCHPO
ID ALF1_SCHPO Reviewed; 660 AA.
AC O43078;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=ATPase-like fidgetin;
DE AltName: Full=Protein sur2;
GN Name=alf1; Synonyms=sur2; ORFNames=SPBC947.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. {ECO:0000305}.
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DR EMBL; CU329671; CAA17029.1; -; Genomic_DNA.
DR PIR; T40781; T40781.
DR RefSeq; NP_595275.1; NM_001021182.2.
DR AlphaFoldDB; O43078; -.
DR SMR; O43078; -.
DR BioGRID; 277763; 43.
DR STRING; 4896.SPBC947.01.1; -.
DR iPTMnet; O43078; -.
DR SwissPalm; O43078; -.
DR MaxQB; O43078; -.
DR PaxDb; O43078; -.
DR PRIDE; O43078; -.
DR EnsemblFungi; SPBC947.01.1; SPBC947.01.1:pep; SPBC947.01.
DR GeneID; 2541249; -.
DR KEGG; spo:SPBC947.01; -.
DR PomBase; SPBC947.01; -.
DR VEuPathDB; FungiDB:SPBC947.01; -.
DR eggNOG; KOG0740; Eukaryota.
DR HOGENOM; CLU_000688_15_1_1; -.
DR InParanoid; O43078; -.
DR OMA; NGLYDNC; -.
DR PRO; PR:O43078; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0032153; C:cell division site; HDA:PomBase.
DR GO; GO:0051286; C:cell tip; HDA:PomBase.
DR GO; GO:0036391; C:medial cortex septin ring; IDA:PomBase.
DR GO; GO:0035841; C:new growing cell tip; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0035840; C:old growing cell tip; IDA:PomBase.
DR GO; GO:0005524; F:ATP binding; ISM:PomBase.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISM:PomBase.
DR GO; GO:0008568; F:microtubule severing ATPase activity; ISS:PomBase.
DR GO; GO:0001578; P:microtubule bundle formation; ISS:PomBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Vps4_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..660
FT /note="ATPase-like fidgetin"
FT /id="PRO_0000290650"
FT REGION 141..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..166
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..264
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 419..426
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 177
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 660 AA; 71733 MW; 908080909AC2826C CRC64;
MDSDLDRILP IASRALLCEG NRDWAGAYVS YCKVLEEMKK SSAARDRMGL GPLTGAEACS
WNGLYDNCLS KASKLRKTIL ESEMERQNYQ LAAKLSKKAP VDLHPLRPVR SQTPAYTPMT
TRMMYRQTRG AQSEVNLSTP KQIYSKHSPP STSTSSIVSS SYGDAPSYLA PSKPNRSPPL
KPEDPFASFN SSASAIAAAS KSAAASASAL SSDTGRSATM NSTTFPTAMK SQSTTKPTLS
NSVSSPSIQV SNNQNANNST PLSFHAPIPP LHVPAVPLTS ASHSSSDGKS RKHPSPYKPY
LNSSHDTLGS STRPSSADTA GSPATSPPAT ADSKTIVSKT ISASTTQQTE PLQQTTPSSD
FEYAIMNEII SNHEPVYWSD IAGLDDAKNS LKEAVIYPFL RPELFQGLRE PVQGMLLFGP
PGTGKTMLAR AVATEAKATF FSISASSLTS KYLGDSEKLV RALFEVAKRQ TCSVIFVDEI
DSILSARNDS GNEHESSRRL KTEFLIQWSS LTNAAPDKQT GHSPRVLVLA ATNLPWCIDE
AARRRFVKRT YIPLPEKETR YKHLSHLLHN QVHCLTEEDL EELVNLTEGY SGSDITALAK
DAAMGPLRNL GDALLTTSAE MIPPISLNHF KASLRTIRPS VSQEGIHRYE EWNKQFGSQR
