ALFC3_ORYSJ
ID ALFC3_ORYSJ Reviewed; 358 AA.
AC Q5N725; Q42476;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Fructose-bisphosphate aldolase 3, cytoplasmic {ECO:0000305};
DE EC=4.1.2.13 {ECO:0000250|UniProtKB:Q9SJQ9};
DE AltName: Full=Aldolase C-1 {ECO:0000303|PubMed:8616263};
DE Short=AldC-1 {ECO:0000303|PubMed:8616263};
GN Name=FBA3 {ECO:0000305};
GN OrderedLocusNames=Os01g0905800 {ECO:0000312|EMBL:BAF07044.2},
GN LOC_Os01g67860 {ECO:0000305};
GN ORFNames=B1417F08.1-1 {ECO:0000312|EMBL:BAD82731.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=8616263; DOI=10.1007/bf00020125;
RA Nakamura H., Satoh W., Hidaka S., Kagaya Y., Ejiri S., Tsutsumi K.;
RT "Genomic structure of the rice aldolase isozyme C-1 gene and its regulation
RT through a Ca 2+ -mediated protein kinase-phosphatase pathway.";
RL Plant Mol. Biol. 30:381-385(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA Yoon U.H., Kim Y.H.;
RT "Molecular cloning of fructose-bisphosphate aldolase genes in rice seeds.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo;
RA Yoon U.H., Kim Y.H.;
RT "Structural and expression analysis of germinating seed genes in Oryza
RT sativa L.";
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nakdong; TISSUE=Leaf;
RA Chang A.;
RT "Oryza sativa japonica group fructose-bisphosphate aldolase mRNA.";
RL Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [8]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Fructose-bisphosphate aldolase that plays a key role in
CC glycolysis and gluconeogenesis. {ECO:0000250|UniProtKB:Q9SJQ9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-
CC phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729,
CC ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13;
CC Evidence={ECO:0000250|UniProtKB:Q9SJQ9};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 4/4.
CC {ECO:0000305}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q944G9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q9SJQ9}.
CC -!- SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase
CC family. {ECO:0000305}.
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DR EMBL; D50301; BAA08830.1; -; mRNA.
DR EMBL; D50307; BAA08845.1; -; Genomic_DNA.
DR EMBL; EF122473; ABL74560.1; -; mRNA.
DR EMBL; GQ848028; ADM86841.1; -; mRNA.
DR EMBL; JN208224; AEV58398.1; -; mRNA.
DR EMBL; AP006531; BAD82731.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF07044.2; -; Genomic_DNA.
DR EMBL; AP014957; BAS75791.1; -; Genomic_DNA.
DR EMBL; AK104719; BAG96902.1; -; mRNA.
DR PIR; S65073; S65073.
DR RefSeq; XP_015613809.1; XM_015758323.1.
DR AlphaFoldDB; Q5N725; -.
DR SMR; Q5N725; -.
DR STRING; 4530.OS01T0905800-01; -.
DR CarbonylDB; Q5N725; -.
DR PaxDb; Q5N725; -.
DR PRIDE; Q5N725; -.
DR EnsemblPlants; Os01t0905800-01; Os01t0905800-01; Os01g0905800.
DR EnsemblPlants; Os01t0905800-03; Os01t0905800-03; Os01g0905800.
DR GeneID; 4325027; -.
DR Gramene; Os01t0905800-01; Os01t0905800-01; Os01g0905800.
DR Gramene; Os01t0905800-03; Os01t0905800-03; Os01g0905800.
DR KEGG; osa:4325027; -.
DR eggNOG; KOG1557; Eukaryota.
DR HOGENOM; CLU_031243_0_2_1; -.
DR InParanoid; Q5N725; -.
DR OMA; FGSCHAY; -.
DR OrthoDB; 799973at2759; -.
DR PlantReactome; R-OSA-1119519; Calvin cycle.
DR PlantReactome; R-OSA-1119570; Cytosolic glycolysis.
DR UniPathway; UPA00109; UER00183.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5N725; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004332; F:fructose-bisphosphate aldolase activity; ISS:UniProtKB.
DR GO; GO:0030388; P:fructose 1,6-bisphosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006094; P:gluconeogenesis; ISS:UniProtKB.
DR GO; GO:0006096; P:glycolytic process; ISS:UniProtKB.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR029768; Aldolase_I_AS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR000741; FBA_I.
DR PANTHER; PTHR11627; PTHR11627; 1.
DR Pfam; PF00274; Glycolytic; 1.
DR PROSITE; PS00158; ALDOLASE_CLASS_I; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Glycolysis; Lyase; Reference proteome; Schiff base.
FT CHAIN 1..358
FT /note="Fructose-bisphosphate aldolase 3, cytoplasmic"
FT /id="PRO_0000437243"
FT ACT_SITE 183
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P00883"
FT ACT_SITE 225
FT /note="Schiff-base intermediate with dihydroxyacetone-P"
FT /evidence="ECO:0000250|UniProtKB:P00883"
FT BINDING 39
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P00883"
FT BINDING 266..268
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P00883"
FT BINDING 298
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P00883"
FT SITE 358
FT /note="Necessary for preference for fructose 1,6-
FT bisphosphate over fructose 1-phosphate"
FT /evidence="ECO:0000250|UniProtKB:P00883"
SQ SEQUENCE 358 AA; 38823 MW; C836F3CB74F33A1F CRC64;
MSAYCGKYKD ELIKNAAYIG TPGKGILAAD ESTGTIGKRF ASINVENVEE NRRSLRELLF
TTPGALQHLS GVILFEETLY QKTKDGKPFV DVLKEGGVLP GIKVDKGTVE VAGTNKETTT
QGHDDLGKRC AKYYEAGARF AKWRAVLKIG PNEPSQLSID LNAQGLARYA IICQENGLVP
IVEPEILVDG SHDIERCAYV TEKVLAACYK ALNEHHVLLE GSLLKPNMVT PGSESKKVSP
QLIAEYTVRA LQRTVPAAVP AIVFLSGGQS EEEATVNLNA MNKLSTKKPW ALSFSFGRAL
QQSTLKAWGG KTENVVKAQK AFITRCKANS EATLGTYQGD AVLGEGASES LHVKDYKY
