FUB11_FUSOX
ID FUB11_FUSOX Reviewed; 593 AA.
AC A0A0B5EMG9;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=Efflux pump FUBT {ECO:0000305};
DE AltName: Full=Fusaric acid biosynthesis protein T {ECO:0000303|PubMed:25627440};
DE AltName: Full=Fusaric acid transporter {ECO:0000303|PubMed:25627440};
GN Name=FUBT {ECO:0000303|PubMed:25627440};
OS Fusarium oxysporum (Fusarium vascular wilt).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium oxysporum species complex.
OX NCBI_TaxID=5507;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=AuK24232;
RX PubMed=25627440; DOI=10.1099/mic.0.000043;
RA Crutcher F.K., Liu J., Puckhaber L.S., Stipanovic R.D., Bell A.A.,
RA Nichols R.L.;
RT "FUBT, a putative MFS transporter, promotes secretion of fusaric acid in
RT the cotton pathogen Fusarium oxysporum f. sp. vasinfectum.";
RL Microbiology 161:875-883(2015).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=16868776; DOI=10.1007/s00425-006-0345-6;
RA Samadi L., Shahsavan Behboodi B.;
RT "Fusaric acid induces apoptosis in saffron root-tip cells: roles of
RT caspase-like activity, cytochrome c, and H2O2.";
RL Planta 225:223-234(2006).
RN [3]
RP BIOTECHNOLOGY.
RX PubMed=17927749; DOI=10.1111/j.1365-2672.2007.03581.x;
RA Son S.W., Kim H.Y., Choi G.J., Lim H.K., Jang K.S., Lee S.O., Lee S.,
RA Sung N.D., Kim J.C.;
RT "Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete
RT activity against Phytophthora infestans.";
RL J. Appl. Microbiol. 104:692-698(2008).
RN [4]
RP BIOTECHNOLOGY.
RX PubMed=21811925; DOI=10.1007/s12272-011-0716-9;
RA Pan J.H., Chen Y., Huang Y.H., Tao Y.W., Wang J., Li Y., Peng Y., Dong T.,
RA Lai X.M., Lin Y.C.;
RT "Antimycobacterial activity of fusaric acid from a mangrove endophyte and
RT its metal complexes.";
RL Arch. Pharm. Res. 34:1177-1181(2011).
RN [5]
RP BIOTECHNOLOGY.
RX PubMed=20955724; DOI=10.1016/j.toxicon.2010.10.006;
RA Stipanovic R.D., Puckhaber L.S., Liu J., Bell A.A.;
RT "Phytotoxicity of fusaric acid and analogs to cotton.";
RL Toxicon 57:176-178(2011).
RN [6]
RP BIOTECHNOLOGY.
RX PubMed=22864988; DOI=10.1055/s-0032-1315146;
RA Boonman N., Prachya S., Boonmee A., Kittakoop P., Wiyakrutta S.,
RA Sriubolmas N., Warit S., Dharmkrong-At Chusattayanond A.;
RT "In vitro acanthamoebicidal activity of fusaric acid and dehydrofusaric
RT acid from an endophytic fungus Fusarium sp. Tlau3.";
RL Planta Med. 78:1562-1567(2012).
RN [7]
RP BIOTECHNOLOGY.
RX PubMed=23838885; DOI=10.1007/s00425-013-1928-7;
RA Jiao J., Zhou B., Zhu X., Gao Z., Liang Y.;
RT "Fusaric acid induction of programmed cell death modulated through nitric
RT oxide signalling in tobacco suspension cells.";
RL Planta 238:727-737(2013).
RN [8]
RP BIOTECHNOLOGY.
RX PubMed=23922960; DOI=10.1371/journal.pone.0070226;
RA Li C., Zuo C., Deng G., Kuang R., Yang Q., Hu C., Sheng O., Zhang S.,
RA Ma L., Wei Y., Yang J., Liu S., Biswas M.K., Viljoen A., Yi G.;
RT "Contamination of bananas with beauvericin and fusaric acid produced by
RT Fusarium oxysporum f. sp. cubense.";
RL PLoS ONE 8:E70226-E70226(2013).
CC -!- FUNCTION: Efflux pump involved in export of fusaric acid, a mycotoxin
CC with low to moderate toxicity to animals and humans, but with high
CC phytotoxic properties (PubMed:25627440). Constitutes a self-protecting
CC mechanism of the fungus against critical levels of FSA within the cell
CC (PubMed:25627440). {ECO:0000269|PubMed:25627440}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:25627440};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Blocks the secretion of fusaric acid and results
CC in decreased resistance to fusaric acid (PubMed:25627440).
CC {ECO:0000269|PubMed:25627440}.
CC -!- BIOTECHNOLOGY: Fusaric acid is phytotoxic to plants such as cotton and
CC banana (PubMed:20955724, PubMed:23922960). It has been shown to induce
CC programmed cell death in plants (PubMed:16868776, PubMed:23838885). In
CC addition to a mild toxicity to animals, fusaric acid exhibits
CC acanthamoebicidal, antioomycete, and antimycobacterial activities
CC (PubMed:17927749, PubMed:22864988, PubMed:21811925).
CC {ECO:0000269|PubMed:16868776, ECO:0000269|PubMed:17927749,
CC ECO:0000269|PubMed:20955724, ECO:0000269|PubMed:21811925,
CC ECO:0000269|PubMed:22864988, ECO:0000269|PubMed:23838885,
CC ECO:0000269|PubMed:23922960}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family.
CC Polyamines/proton antiporter (TC 2.A.1.2.16) subfamily. {ECO:0000305}.
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DR EMBL; KM435073; AJE75875.1; -; Genomic_DNA.
DR EMBL; KM435074; AJE75876.1; -; mRNA.
DR AlphaFoldDB; A0A0B5EMG9; -.
DR VEuPathDB; FungiDB:FOC1_g10002866; -.
DR VEuPathDB; FungiDB:FOC4_g10004164; -.
DR VEuPathDB; FungiDB:FOIG_16462; -.
DR VEuPathDB; FungiDB:FOMG_16506; -.
DR VEuPathDB; FungiDB:FOXG_15236; -.
DR VEuPathDB; FungiDB:FOZG_06207; -.
DR VEuPathDB; FungiDB:HZS61_009085; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..593
FT /note="Efflux pump FUBT"
FT /id="PRO_0000437354"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 503..523
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 570..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..593
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 593 AA; 65110 MW; 59FAA2169D12C4D7 CRC64;
MAIDPQPSSP SLSSETIAND TIGNDNNVNE PSVEPKTQEN QHTVPPRLSR IYSQAHHISQ
SFIDQNYPGE GTTQAPYRIN FLPDDTQNAQ LLPRWKKWAF VLLQSLACLA TTFASSAYSG
GIKQVIRAFG ISQEVATLGI SLYVLGFTFG PLIWAPLSEL YGRKKVFFFT FMVATAFSAG
AAGAGSIASL LVLRFLTGSI GSAPLSNAPA LIADMFDKSE RGLAMCMFSG APFLGPAIGP
IAGGFLGEAA GWRWLHGLMA AFTGVTWIAC TVFIPETYAP YILRKRAQHM SKLTGKVYIS
TLDADKPPSS AAHQLKNALT RPWLLLFKEP IVFITSIYIS IIYGTMYMCF AAFPIVFQKG
RGWSQGIGGL AFTGIVIGVV LSIISFAFED KRYARAAQRR GAPMEPEDRL PPAIMGSLLI
PIGLFWFAWT TFPSVHWIVP IIGTVFFAWG LVLVFMALLN YLIDSYVIFA ASIMAANSAL
RSLFGAAFPL FTRQMYDGLG VQWASSIPAF LALACVPFPF LFYKYGRQIR MKCEYAAEAA
NVLQKMRSLH VTVTEDDAMN EAEEMWRART HNSHASAAHS HGHRRSLSYT RSV
