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FUB4_GIBM7
ID   FUB4_GIBM7              Reviewed;         267 AA.
AC   W7MT28;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Hydrolase FUB4 {ECO:0000303|PubMed:22652150};
DE            EC=3.1.-.- {ECO:0000305|PubMed:25372119};
DE   AltName: Full=Fusaric acid biosynthesis protein 4 {ECO:0000303|PubMed:22652150};
GN   Name=FUB4 {ECO:0000303|PubMed:22652150}; ORFNames=FVEG_12520;
OS   Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot
OS   fungus) (Fusarium verticillioides).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=334819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M3125 / FGSC 7600;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=16868776; DOI=10.1007/s00425-006-0345-6;
RA   Samadi L., Shahsavan Behboodi B.;
RT   "Fusaric acid induces apoptosis in saffron root-tip cells: roles of
RT   caspase-like activity, cytochrome c, and H2O2.";
RL   Planta 225:223-234(2006).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=17927749; DOI=10.1111/j.1365-2672.2007.03581.x;
RA   Son S.W., Kim H.Y., Choi G.J., Lim H.K., Jang K.S., Lee S.O., Lee S.,
RA   Sung N.D., Kim J.C.;
RT   "Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete
RT   activity against Phytophthora infestans.";
RL   J. Appl. Microbiol. 104:692-698(2008).
RN   [4]
RP   BIOTECHNOLOGY.
RX   PubMed=21811925; DOI=10.1007/s12272-011-0716-9;
RA   Pan J.H., Chen Y., Huang Y.H., Tao Y.W., Wang J., Li Y., Peng Y., Dong T.,
RA   Lai X.M., Lin Y.C.;
RT   "Antimycobacterial activity of fusaric acid from a mangrove endophyte and
RT   its metal complexes.";
RL   Arch. Pharm. Res. 34:1177-1181(2011).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=20955724; DOI=10.1016/j.toxicon.2010.10.006;
RA   Stipanovic R.D., Puckhaber L.S., Liu J., Bell A.A.;
RT   "Phytotoxicity of fusaric acid and analogs to cotton.";
RL   Toxicon 57:176-178(2011).
RN   [6]
RP   FUNCTION.
RX   PubMed=22652150; DOI=10.1016/j.fgb.2012.05.010;
RA   Brown D.W., Butchko R.A., Busman M., Proctor R.H.;
RT   "Identification of gene clusters associated with fusaric acid, fusarin, and
RT   perithecial pigment production in Fusarium verticillioides.";
RL   Fungal Genet. Biol. 49:521-532(2012).
RN   [7]
RP   INDUCTION.
RX   PubMed=22713715; DOI=10.1016/j.fgb.2012.06.003;
RA   Butchko R.A., Brown D.W., Busman M., Tudzynski B., Wiemann P.;
RT   "Lae1 regulates expression of multiple secondary metabolite gene clusters
RT   in Fusarium verticillioides.";
RL   Fungal Genet. Biol. 49:602-612(2012).
RN   [8]
RP   BIOTECHNOLOGY.
RX   PubMed=22864988; DOI=10.1055/s-0032-1315146;
RA   Boonman N., Prachya S., Boonmee A., Kittakoop P., Wiyakrutta S.,
RA   Sriubolmas N., Warit S., Dharmkrong-At Chusattayanond A.;
RT   "In vitro acanthamoebicidal activity of fusaric acid and dehydrofusaric
RT   acid from an endophytic fungus Fusarium sp. Tlau3.";
RL   Planta Med. 78:1562-1567(2012).
RN   [9]
RP   BIOTECHNOLOGY.
RX   PubMed=23838885; DOI=10.1007/s00425-013-1928-7;
RA   Jiao J., Zhou B., Zhu X., Gao Z., Liang Y.;
RT   "Fusaric acid induction of programmed cell death modulated through nitric
RT   oxide signalling in tobacco suspension cells.";
RL   Planta 238:727-737(2013).
RN   [10]
RP   BIOTECHNOLOGY.
RX   PubMed=23922960; DOI=10.1371/journal.pone.0070226;
RA   Li C., Zuo C., Deng G., Kuang R., Yang Q., Hu C., Sheng O., Zhang S.,
RA   Ma L., Wei Y., Yang J., Liu S., Biswas M.K., Viljoen A., Yi G.;
RT   "Contamination of bananas with beauvericin and fusaric acid produced by
RT   Fusarium oxysporum f. sp. cubense.";
RL   PLoS ONE 8:E70226-E70226(2013).
RN   [11]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX   PubMed=25372119; DOI=10.1094/mpmi-09-14-0264-r;
RA   Brown D.W., Lee S.H., Kim L.H., Ryu J.G., Lee S., Seo Y., Kim Y.H.,
RA   Busman M., Yun S.H., Proctor R.H., Lee T.;
RT   "Identification of a 12-gene fusaric acid biosynthetic gene cluster in
RT   Fusarium species through comparative and functional genomics.";
RL   Mol. Plant Microbe Interact. 28:319-332(2015).
CC   -!- FUNCTION: Hydrolase; part of the gene cluster that mediates the
CC       biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity
CC       to animals and humans, but with high phytotoxic properties
CC       (PubMed:22652150, PubMed:25372119). L-aspartate is suggested as fusaric
CC       acid amino acid precursor that is activated and further processed to O-
CC       acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and
CC       homoserine O-acetyltransferase FUB5, as well as enzymes of the primary
CC       metabolism (By similarity). The polyketide synthase (PKS) FUB1
CC       generates the triketide trans-2-hexenal which is presumptively released
CC       by the hydrolase FUB4 and linked to the NRPS-bound amino acid precursor
CC       by NAD(P)-dependent dehydrogenase FUB6 (By similarity). FUB1, FUB4, and
CC       the non-canonical NRPS Fub8 may form an enzyme complex (By similarity).
CC       Further processing of the NRPS-bound intermediate might be carried out
CC       by FUB6 and the sulfhydrylase FUB7, enabling a spontaneous
CC       electrocyclization to close the carbon backbone of fusaric acid (By
CC       similarity). Dihydrofusaric acid is likely to be released via reduction
CC       by the thioester reductase (TR) domain of FUB8 whereupon the final
CC       oxidation to fusaric acid may (also) be performed by the FMN-dependent
CC       dehydrogenase FUB9 (By similarity). {ECO:0000250|UniProtKB:S0DRW4,
CC       ECO:0000269|PubMed:22652150, ECO:0000269|PubMed:25372119}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:22652150,
CC       ECO:0000269|PubMed:25372119}.
CC   -!- INDUCTION: Expression is positively regulated by the secondary
CC       metabolism regulator LAE1 (PubMed:22713715).
CC       {ECO:0000269|PubMed:22713715}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of fusaric acid
CC       (PubMed:25372119). {ECO:0000269|PubMed:25372119}.
CC   -!- BIOTECHNOLOGY: Fusaric acid is phytotoxic to plants such as cotton and
CC       banana (PubMed:20955724, PubMed:23922960). It has been shown to induce
CC       programmed cell death in plants (PubMed:16868776, PubMed:23838885). In
CC       addition to a mild toxicity to animals, fusaric acid exhibits
CC       acanthamoebicidal, antioomycete, and antimycobacterial activities
CC       (PubMed:17927749, PubMed:22864988, PubMed:21811925).
CC       {ECO:0000269|PubMed:16868776, ECO:0000269|PubMed:17927749,
CC       ECO:0000269|PubMed:20955724, ECO:0000269|PubMed:21811925,
CC       ECO:0000269|PubMed:22864988, ECO:0000269|PubMed:23838885,
CC       ECO:0000269|PubMed:23922960}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase 3 family. {ECO:0000305}.
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DR   EMBL; DS022261; EWG54261.1; -; Genomic_DNA.
DR   RefSeq; XP_018760452.1; XM_018901860.1.
DR   AlphaFoldDB; W7MT28; -.
DR   SMR; W7MT28; -.
DR   EnsemblFungi; FVEG_12520T0; FVEG_12520T0; FVEG_12520.
DR   GeneID; 30069954; -.
DR   KEGG; fvr:FVEG_12520; -.
DR   VEuPathDB; FungiDB:FVEG_12520; -.
DR   eggNOG; KOG2551; Eukaryota.
DR   HOGENOM; CLU_051938_4_1_1; -.
DR   OMA; YVESEGP; -.
DR   OrthoDB; 1190789at2759; -.
DR   BioCyc; MetaCyc:MON-19344; -.
DR   PHI-base; PHI:3390; -.
DR   Proteomes; UP000009096; Chromosome 3.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005645; FSH_dom.
DR   Pfam; PF03959; FSH1; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Hydrolase FUB4"
FT                   /id="PRO_0000437316"
FT   ACT_SITE        93
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        183
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        243
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
SQ   SEQUENCE   267 AA;  30856 MW;  D28E3A53EB5966E4 CRC64;
     MRFLCLHGYA FSVEVLQQQM EPITAHLPSD WEYEFLEAGM EPTQLMLPNL KQVPKPNSSW
     YNFPYPEDVE EAYERLAAYV ESEGPFDGIW GFSQGGSMAA LLLLMHQAEH PDTPYPFKMA
     IFTSAFLPHS FDNGVISWDL TEKNTLEPAY LPGRIDVSHG KKLDWKKDLH TSIEYDMINA
     VKDELDFPVD LLLRWRPSDI PEKIPVPSVH VRGLKDHYSF VDESVYELFD PDMARKMTHR
     GGHNFPRYNE ELVHFAELII ETVVSLH
 
 
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