ID   FUB6_FUSO4              Reviewed;         357 AA.
AC   A0A0D2YG03;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Dehydrogenase FUB6 {ECO:0000303|PubMed:25372119};
DE            EC=1.-.-.- {ECO:0000305|PubMed:25372119};
DE   AltName: Full=Fusaric acid biosynthesis protein 6 {ECO:0000303|PubMed:25372119};
GN   Name=FUB6 {ECO:0000303|PubMed:25372119}; ORFNames=FOXG_15241;
OS   Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935
OS   / NRRL 34936) (Fusarium vascular wilt of tomato).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium oxysporum species complex.
OX   NCBI_TaxID=426428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936;
RG   EnsemblFungi;
RL   Submitted (MAR-2015) to UniProtKB.
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=16868776; DOI=10.1007/s00425-006-0345-6;
RA   Samadi L., Shahsavan Behboodi B.;
RT   "Fusaric acid induces apoptosis in saffron root-tip cells: roles of
RT   caspase-like activity, cytochrome c, and H2O2.";
RL   Planta 225:223-234(2006).
RN   [4]
RP   BIOTECHNOLOGY.
RX   PubMed=17927749; DOI=10.1111/j.1365-2672.2007.03581.x;
RA   Son S.W., Kim H.Y., Choi G.J., Lim H.K., Jang K.S., Lee S.O., Lee S.,
RA   Sung N.D., Kim J.C.;
RT   "Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete
RT   activity against Phytophthora infestans.";
RL   J. Appl. Microbiol. 104:692-698(2008).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=21811925; DOI=10.1007/s12272-011-0716-9;
RA   Pan J.H., Chen Y., Huang Y.H., Tao Y.W., Wang J., Li Y., Peng Y., Dong T.,
RA   Lai X.M., Lin Y.C.;
RT   "Antimycobacterial activity of fusaric acid from a mangrove endophyte and
RT   its metal complexes.";
RL   Arch. Pharm. Res. 34:1177-1181(2011).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=20955724; DOI=10.1016/j.toxicon.2010.10.006;
RA   Stipanovic R.D., Puckhaber L.S., Liu J., Bell A.A.;
RT   "Phytotoxicity of fusaric acid and analogs to cotton.";
RL   Toxicon 57:176-178(2011).
RN   [7]
RP   BIOTECHNOLOGY.
RX   PubMed=22864988; DOI=10.1055/s-0032-1315146;
RA   Boonman N., Prachya S., Boonmee A., Kittakoop P., Wiyakrutta S.,
RA   Sriubolmas N., Warit S., Dharmkrong-At Chusattayanond A.;
RT   "In vitro acanthamoebicidal activity of fusaric acid and dehydrofusaric
RT   acid from an endophytic fungus Fusarium sp. Tlau3.";
RL   Planta Med. 78:1562-1567(2012).
RN   [8]
RP   BIOTECHNOLOGY.
RX   PubMed=23838885; DOI=10.1007/s00425-013-1928-7;
RA   Jiao J., Zhou B., Zhu X., Gao Z., Liang Y.;
RT   "Fusaric acid induction of programmed cell death modulated through nitric
RT   oxide signalling in tobacco suspension cells.";
RL   Planta 238:727-737(2013).
RN   [9]
RP   BIOTECHNOLOGY.
RX   PubMed=23922960; DOI=10.1371/journal.pone.0070226;
RA   Li C., Zuo C., Deng G., Kuang R., Yang Q., Hu C., Sheng O., Zhang S.,
RA   Ma L., Wei Y., Yang J., Liu S., Biswas M.K., Viljoen A., Yi G.;
RT   "Contamination of bananas with beauvericin and fusaric acid produced by
RT   Fusarium oxysporum f. sp. cubense.";
RL   PLoS ONE 8:E70226-E70226(2013).
RN   [10]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX   PubMed=25372119; DOI=10.1094/mpmi-09-14-0264-r;
RA   Brown D.W., Lee S.H., Kim L.H., Ryu J.G., Lee S., Seo Y., Kim Y.H.,
RA   Busman M., Yun S.H., Proctor R.H., Lee T.;
RT   "Identification of a 12-gene fusaric acid biosynthetic gene cluster in
RT   Fusarium species through comparative and functional genomics.";
RL   Mol. Plant Microbe Interact. 28:319-332(2015).
CC   -!- FUNCTION: Dehydrogenase; part of the gene cluster that mediates the
CC       biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity
CC       to animals and humans, but with high phytotoxic properties
CC       (PubMed:25372119). L-aspartate is suggested as fusaric acid amino acid
CC       precursor that is activated and further processed to O-acetyl-L-
CC       homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-
CC       acetyltransferase FUB5, as well as enzymes of the primary metabolism
CC       (By similarity). The polyketide synthase (PKS) FUB1 generates the
CC       triketide trans-2-hexenal which is presumptively released by the
CC       hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by
CC       NAD(P)-dependent dehydrogenase FUB6 (By similarity). FUB1, FUB4, and
CC       the non-canonical NRPS Fub8 may form an enzyme complex (By similarity).
CC       Further processing of the NRPS-bound intermediate might be carried out
CC       by FUB6 and the O-acetylhomoserine FUB7, enabling a spontaneous
CC       electrocyclization to close the carbon backbone of fusaric acid (By
CC       similarity). Dihydrofusaric acid is likely to be released via reduction
CC       by the thioester reductase (TR) domain of FUB8 whereupon the final
CC       oxidation to fusaric acid may (also) be performed by the FMN-dependent
CC       dehydrogenase FUB9 (By similarity). {ECO:0000250|UniProtKB:S0DRW9,
CC       ECO:0000269|PubMed:25372119}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:25372119}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of fusaric acid
CC       (PubMed:25372119). {ECO:0000269|PubMed:25372119}.
CC   -!- BIOTECHNOLOGY: Fusaric acid is phytotoxic to plants such as cotton and
CC       banana (PubMed:20955724, PubMed:23922960). It has been shown to induce
CC       programmed cell death in plants (PubMed:16868776, PubMed:23838885). In
CC       addition to a mild toxicity to animals, fusaric acid exhibits
CC       acanthamoebicidal, antioomycete, and antimycobacterial activities
CC       (PubMed:17927749, PubMed:22864988, PubMed:21811925).
CC       {ECO:0000269|PubMed:16868776, ECO:0000269|PubMed:17927749,
CC       ECO:0000269|PubMed:20955724, ECO:0000269|PubMed:21811925,
CC       ECO:0000269|PubMed:22864988, ECO:0000269|PubMed:23838885,
CC       ECO:0000269|PubMed:23922960}.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. Quinone oxidoreductase subfamily. {ECO:0000305}.
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DR   EMBL; DS231721; KNB17114.1; -; Genomic_DNA.
DR   RefSeq; XP_018255159.1; XM_018395326.1.
DR   AlphaFoldDB; A0A0D2YG03; -.
DR   SMR; A0A0D2YG03; -.
DR   EnsemblFungi; FOXG_15241T0; FOXG_15241P0; FOXG_15241.
DR   EnsemblFungi; KNB17114; KNB17114; FOXG_15241.
DR   GeneID; 28956317; -.
DR   KEGG; fox:FOXG_15241; -.
DR   VEuPathDB; FungiDB:FOXG_15241; -.
DR   OMA; HQRDRMR; -.
DR   Proteomes; UP000009097; Chromosome 5.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR041694; ADH_N_2.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR045010; MDR_fam.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   PANTHER; PTHR43205; PTHR43205; 1.
DR   Pfam; PF16884; ADH_N_2; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..357
FT                   /note="Dehydrogenase FUB6"
FT                   /id="PRO_0000437335"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   357 AA;  39132 MW;  057FF3270200CF2C CRC64;
     MGGEVSNKTW VFKQSPSGLP EPGVHTAFED RPLSLVAPPG GLVINLLTAG LDPHQRDRMR
     GAGNVDYVPG YEVNEPITNF SIAKVIRSDN AAFEEGSLIA GSLPIAEYGI IPKELIDARA
     MASPLVWKVS NDYNLDVKHY VGTLGLAGMT AWNSFYGLVK PVKGETIWVN AASSSVGEVV
     VQLAKIEGMK VIASVSSDDK LDYVVNELGA DVGFNYRKEP VGKALKRLAP DGLDVVFENV
     GGDHFQAAIE NMKWFGRIIS CGTASQYNKP VEEQYGVTNL SEIFRRRIKI QGFIFWDDNI
     YTDNIENFKA TMPKWVSEGK IKSRYTQFEG IEQADKAFLS MFTGGSHGKT VLKISDP