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FUB6_GIBF5
ID   FUB6_GIBF5              Reviewed;         337 AA.
AC   S0DRW9;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2013, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Dehydrogenase FUB6 {ECO:0000303|PubMed:26662839};
DE            EC=1.-.-.- {ECO:0000305|PubMed:26662839};
DE   AltName: Full=Fusaric acid biosynthesis protein 6 {ECO:0000303|PubMed:26662839};
GN   Name=FUB6 {ECO:0000303|PubMed:26662839}; ORFNames=FFUJ_02113;
OS   Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS   and foot rot disease fungus) (Fusarium fujikuroi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=1279085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX   PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA   Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA   Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA   Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA   Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA   Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA   Tudzynski B.;
RT   "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT   Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT   novel metabolites.";
RL   PLoS Pathog. 9:E1003475-E1003475(2013).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=16868776; DOI=10.1007/s00425-006-0345-6;
RA   Samadi L., Shahsavan Behboodi B.;
RT   "Fusaric acid induces apoptosis in saffron root-tip cells: roles of
RT   caspase-like activity, cytochrome c, and H2O2.";
RL   Planta 225:223-234(2006).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=17927749; DOI=10.1111/j.1365-2672.2007.03581.x;
RA   Son S.W., Kim H.Y., Choi G.J., Lim H.K., Jang K.S., Lee S.O., Lee S.,
RA   Sung N.D., Kim J.C.;
RT   "Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete
RT   activity against Phytophthora infestans.";
RL   J. Appl. Microbiol. 104:692-698(2008).
RN   [4]
RP   BIOTECHNOLOGY.
RX   PubMed=21811925; DOI=10.1007/s12272-011-0716-9;
RA   Pan J.H., Chen Y., Huang Y.H., Tao Y.W., Wang J., Li Y., Peng Y., Dong T.,
RA   Lai X.M., Lin Y.C.;
RT   "Antimycobacterial activity of fusaric acid from a mangrove endophyte and
RT   its metal complexes.";
RL   Arch. Pharm. Res. 34:1177-1181(2011).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=20955724; DOI=10.1016/j.toxicon.2010.10.006;
RA   Stipanovic R.D., Puckhaber L.S., Liu J., Bell A.A.;
RT   "Phytotoxicity of fusaric acid and analogs to cotton.";
RL   Toxicon 57:176-178(2011).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=22864988; DOI=10.1055/s-0032-1315146;
RA   Boonman N., Prachya S., Boonmee A., Kittakoop P., Wiyakrutta S.,
RA   Sriubolmas N., Warit S., Dharmkrong-At Chusattayanond A.;
RT   "In vitro acanthamoebicidal activity of fusaric acid and dehydrofusaric
RT   acid from an endophytic fungus Fusarium sp. Tlau3.";
RL   Planta Med. 78:1562-1567(2012).
RN   [7]
RP   BIOTECHNOLOGY.
RX   PubMed=23838885; DOI=10.1007/s00425-013-1928-7;
RA   Jiao J., Zhou B., Zhu X., Gao Z., Liang Y.;
RT   "Fusaric acid induction of programmed cell death modulated through nitric
RT   oxide signalling in tobacco suspension cells.";
RL   Planta 238:727-737(2013).
RN   [8]
RP   BIOTECHNOLOGY.
RX   PubMed=23922960; DOI=10.1371/journal.pone.0070226;
RA   Li C., Zuo C., Deng G., Kuang R., Yang Q., Hu C., Sheng O., Zhang S.,
RA   Ma L., Wei Y., Yang J., Liu S., Biswas M.K., Viljoen A., Yi G.;
RT   "Contamination of bananas with beauvericin and fusaric acid produced by
RT   Fusarium oxysporum f. sp. cubense.";
RL   PLoS ONE 8:E70226-E70226(2013).
RN   [9]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX   PubMed=26662839; DOI=10.1111/1462-2920.13150;
RA   Studt L., Janevska S., Niehaus E.M., Burkhardt I., Arndt B., Sieber C.M.,
RA   Humpf H.U., Dickschat J.S., Tudzynski B.;
RT   "Two separate key enzymes and two pathway-specific transcription factors
RT   are involved in fusaric acid biosynthesis in Fusarium fujikuroi.";
RL   Environ. Microbiol. 18:936-956(2016).
CC   -!- FUNCTION: Dehydrogenase; part of the gene cluster that mediates the
CC       biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity
CC       to animals and humans, but with high phytotoxic properties
CC       (PubMed:26662839). L-aspartate is suggested as fusaric acid amino acid
CC       precursor that is activated and further processed to O-acetyl-L-
CC       homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-
CC       acetyltransferase FUB5, as well as enzymes of the primary metabolism
CC       (PubMed:26662839). The polyketide synthase (PKS) FUB1 generates the
CC       triketide trans-2-hexenal which is presumptively released by the
CC       hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by
CC       NAD(P)-dependent dehydrogenase FUB6 (PubMed:26662839). FUB1, FUB4, and
CC       the non-canonical NRPS Fub8 may form an enzyme complex
CC       (PubMed:26662839). Further processing of the NRPS-bound intermediate
CC       might be carried out by FUB6 and the O-acetylhomoserine FUB7, enabling
CC       a spontaneous electrocyclization to close the carbon backbone of
CC       fusaric acid (PubMed:26662839). Dihydrofusaric acid is likely to be
CC       released via reduction by the thioester reductase (TR) domain of FUB8
CC       whereupon the final oxidation to fusaric acid may (also) be performed
CC       by the FMN-dependent dehydrogenase FUB9 (PubMed:26662839).
CC       {ECO:0000269|PubMed:26662839}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:26662839}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of fusaric acid, but leads
CC       to the accumulation of trans-2-hexenal (PubMed:26662839).
CC       {ECO:0000269|PubMed:26662839}.
CC   -!- BIOTECHNOLOGY: Fusaric acid is phytotoxic to plants such as cotton and
CC       banana (PubMed:20955724, PubMed:23922960). It has been shown to induce
CC       programmed cell death in plants (PubMed:16868776, PubMed:23838885). In
CC       addition to a mild toxicity to animals, fusaric acid exhibits
CC       acanthamoebicidal, antioomycete, and antimycobacterial activities
CC       (PubMed:17927749, PubMed:22864988, PubMed:21811925).
CC       {ECO:0000269|PubMed:16868776, ECO:0000269|PubMed:17927749,
CC       ECO:0000269|PubMed:20955724, ECO:0000269|PubMed:21811925,
CC       ECO:0000269|PubMed:22864988, ECO:0000269|PubMed:23838885,
CC       ECO:0000269|PubMed:23922960}.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. Quinone oxidoreductase subfamily. {ECO:0000305}.
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DR   EMBL; HF679025; CCT65191.1; -; Genomic_DNA.
DR   AlphaFoldDB; S0DRW9; -.
DR   SMR; S0DRW9; -.
DR   EnsemblFungi; CCT65191; CCT65191; FFUJ_02113.
DR   VEuPathDB; FungiDB:FFUJ_02113; -.
DR   HOGENOM; CLU_026673_29_1_1; -.
DR   Proteomes; UP000016800; Chromosome 3.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   InterPro; IPR041694; ADH_N_2.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR045010; MDR_fam.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   PANTHER; PTHR43205; PTHR43205; 1.
DR   Pfam; PF16884; ADH_N_2; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..337
FT                   /note="Dehydrogenase FUB6"
FT                   /id="PRO_0000437333"
SQ   SEQUENCE   337 AA;  37108 MW;  41728BBE0CAEB82A CRC64;
     MGGEVSNKTW VFKKSPSSLP EPGVHTAFED RPLSLVAPPG GLVIKLLTAG LDPHQRDRMR
     GAGNVDYVPG YEVNEPITNF SIAKVIRSDN DVFEEGSLIA GSLPIAEYGI IPRELIDARA
     MASPLVWKVS NDYNLDVKHY VGTLGLAGMT AWNSFYGLVK AVKGETIWVN AASSSVGEVV
     VQLAKIEGMK VIASVSSDDK LDYVVNELGA DVGFNYRKEP VGKALKPAIE NMKWFGRIIS
     CGTASQYNKP VEEQYGVTNL SEIFRRRIKI QGFIFWDDNI YTDNIENFKA TMPKWVSEGK
     IKSRYTQFEG IEQADKAFLS MFTGGSHGKT VLKISDP
 
 
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