FUCT1_DROME
ID FUCT1_DROME Reviewed; 337 AA.
AC Q9VHT4; Q53YF6;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=GDP-fucose transporter 1;
DE AltName: Full=Neuronally altered carbohydrate;
DE AltName: Full=Solute carrier family 35 member C1 homolog;
GN Name=nac; Synonyms=Gfr; ORFNames=CG9620;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19948734; DOI=10.1074/jbc.m109.016964;
RA Ishikawa H.O., Ayukawa T., Nakayama M., Higashi S., Kamiyama S.,
RA Nishihara S., Aoki K., Ishida N., Sanai Y., Matsuno K.;
RT "Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen
RT function redundantly in the O-fucosylation of Notch in Drosophila.";
RL J. Biol. Chem. 285:4122-4129(2010).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF SER-29.
RX PubMed=22745127; DOI=10.1074/jbc.m112.379313;
RA Geisler C., Kotu V., Sharrow M., Rendic D., Poltl G., Tiemeyer M.,
RA Wilson I.B., Jarvis D.L.;
RT "The Drosophila neurally altered carbohydrate mutant has a defective Golgi
RT GDP-fucose transporter.";
RL J. Biol. Chem. 287:29599-29609(2012).
CC -!- FUNCTION: Involved in GDP-fucose import from the cytoplasm into the
CC Golgi lumen. Plays a major role in the fucosylation of N-glycans.
CC Functions redundantly with Efr in the O-fucosylation of Notch,
CC positively regulating Notch signaling. {ECO:0000269|PubMed:19948734,
CC ECO:0000269|PubMed:22745127}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:19948734, ECO:0000269|PubMed:22745127}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:19948734,
CC ECO:0000269|PubMed:22745127}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35C subfamily.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF54215.1; -; Genomic_DNA.
DR EMBL; BT003501; AAO39505.1; -; mRNA.
DR RefSeq; NP_649782.1; NM_141525.2.
DR AlphaFoldDB; Q9VHT4; -.
DR SMR; Q9VHT4; -.
DR STRING; 7227.FBpp0081352; -.
DR TCDB; 2.A.7.16.2; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q9VHT4; -.
DR PRIDE; Q9VHT4; -.
DR DNASU; 40981; -.
DR EnsemblMetazoa; FBtr0081863; FBpp0081352; FBgn0265351.
DR GeneID; 40981; -.
DR KEGG; dme:Dmel_CG9620; -.
DR CTD; 40981; -.
DR FlyBase; FBgn0265351; nac.
DR VEuPathDB; VectorBase:FBgn0265351; -.
DR eggNOG; KOG1442; Eukaryota.
DR GeneTree; ENSGT00390000013315; -.
DR HOGENOM; CLU_044894_1_0_1; -.
DR InParanoid; Q9VHT4; -.
DR OMA; GLQIKYT; -.
DR OrthoDB; 870214at2759; -.
DR PhylomeDB; Q9VHT4; -.
DR Reactome; R-DME-6787639; GDP-fucose biosynthesis.
DR Reactome; R-DME-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 40981; 0 hits in 1 CRISPR screen.
DR ChiTaRS; nac; fly.
DR GenomeRNAi; 40981; -.
DR PRO; PR:Q9VHT4; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0265351; Expressed in embryonic/larval hemocyte (Drosophila) and 27 other tissues.
DR Genevisible; Q9VHT4; DM.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005798; C:Golgi-associated vesicle; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005457; F:GDP-fucose transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0036085; P:GDP-fucose import into Golgi lumen; IDA:FlyBase.
DR GO; GO:0015783; P:GDP-fucose transmembrane transport; IDA:FlyBase.
DR GO; GO:0048193; P:Golgi vesicle transport; IDA:FlyBase.
DR GO; GO:0007625; P:grooming behavior; IMP:FlyBase.
DR GO; GO:0036071; P:N-glycan fucosylation; IMP:FlyBase.
DR GO; GO:0036066; P:protein O-linked fucosylation; IMP:FlyBase.
DR InterPro; IPR029666; GDP-fucose_transp.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR PANTHER; PTHR11132:SF255; PTHR11132:SF255; 1.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..337
FT /note="GDP-fucose transporter 1"
FT /id="PRO_0000213393"
FT TRANSMEM 13..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 29
FT /note="S->L: In nac1; abolishes GDP-fucose transport into
FT the Golgi lumen. Shows lack of both core alpha-1,3- and
FT alpha-1,6-linked fucose residues on N-glycans. Reduces
FT neural expression of the HRP epitope. Does not affect
FT subcellular localization to the Golgi apparatus."
FT /evidence="ECO:0000269|PubMed:22745127"
SQ SEQUENCE 337 AA; 38107 MW; 1FB7E9C1EC280AD6 CRC64;
MYKNLEEHNR LVNKYLKIFF VVSLYWCTSI LTVFVNKHLL SSDTVNLGAP LFMSWFQCVV
STVICFVASR LSRKYPSVFT FPEGNPLDID TFRKILPLSV LYTLMIGANN LSLSYVTVAF
YYIGRSLTTV FSVVLTYVIL RQRTSFKCLL CCGAIVVGFW LGVDQESLTE VFSWRGTIFG
VLSSLALAMF SIQTKKSLGY VNQEVWLLSY YNNLYSTLLF LPLIIINGEL ESIITYPHLW
ASWFWAAMTL SGLCGFAIGF VTALEIKVTS ALTHNISGTA KACAQTVIAT QYYHDVRSAL
WWTSNVVVLV ASAAYTRVKQ LEMMRQHQQR STATQKA
