ID   FUMA_GEOSE              Reviewed;         514 AA.
AC   Q04718;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Putative fumarate hydratase class I;
DE            Short=Fumarase;
DE            EC=4.2.1.2;
GN   Name=fumA;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 2334 / Var. Non-diastaticus;
RX   PubMed=8473853; DOI=10.1099/00221287-139-3-403;
RA   Reaney S.K., Bungard S.J., Guest J.R.;
RT   "Molecular and enzymological evidence for two classes of fumarase in
RT   Bacillus stearothermophilus (var. non-diastaticus).";
RL   J. Gen. Microbiol. 139:403-416(1993).
CC   -!- FUNCTION: Catalyzes the reversible hydration of fumarate to (S)-malate.
CC       {ECO:0000250|UniProtKB:P0AC33}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P0AC33};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250|UniProtKB:P0AC33};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:P0AC33};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate
CC       from fumarate: step 1/1. {ECO:0000250|UniProtKB:P0AC33}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P0AC33}.
CC   -!- SIMILARITY: Belongs to the class-I fumarase family. {ECO:0000305}.
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DR   EMBL; L05611; AAA72317.1; -; Genomic_DNA.
DR   PIR; A47692; A47692.
DR   AlphaFoldDB; Q04718; -.
DR   SMR; Q04718; -.
DR   UniPathway; UPA00223; UER01007.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.130.10; -; 1.
DR   InterPro; IPR004646; Fe-S_hydro-lyase_TtdA-typ_cat.
DR   InterPro; IPR004647; Fe-S_hydro-lyase_TtdB-typ_cat.
DR   InterPro; IPR036660; Fe-S_hydroAse_TtdB_cat_sf.
DR   InterPro; IPR011167; Fe_dep_fumarate_hydratase.
DR   Pfam; PF05681; Fumerase; 1.
DR   Pfam; PF05683; Fumerase_C; 1.
DR   PIRSF; PIRSF001394; Fe_dep_fumar_hy; 1.
DR   SUPFAM; SSF117457; SSF117457; 1.
DR   TIGRFAMs; TIGR00722; ttdA_fumA_fumB; 1.
DR   TIGRFAMs; TIGR00723; ttdB_fumA_fumB; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Lyase; Metal-binding; Tricarboxylic acid cycle.
FT   CHAIN           1..514
FT                   /note="Putative fumarate hydratase class I"
FT                   /id="PRO_0000195655"
FT   BINDING         62
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
FT   BINDING         187
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
FT   BINDING         274
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:E9AE57"
SQ   SEQUENCE   514 AA;  56792 MW;  238F2890E8CC54E1 CRC64;
     MEKLYESMYQ LIVETSTKLP KDVRRAILKA KQRENAGTRA AMSLATITEN IKMADENVSP
     ICQDTGLPTF KIKVPVGINQ IQIKETIKKA IAQATKDGKL RPNSVDSLTG ENSGDNLGEG
     LPVVKFEQWE KDYMDVRLIL KGGGCENKNI QYSLPCELEG LGRAGRDLDG IRKCILHAVY
     QAQGQGCSAG FIGVGIGGDR SAGYDLAKEQ LFREVDDVNP NEELRQLEEY IMENANKLGI
     GTMGFGGETT LLGCKVGAMH RIPASFFVSV AYNCWAFRRL GVHIDPNTGE IIKWLYQDGE
     DVDFQENAAQ EEHLASTDSE RRVITLQAPI TEEQIRELKV GDVVRINGII YTGRDAIHKY
     LMDHDAPVDL NGQIIYHCGP VMLKDENGNW EVKAAGPTTS IREEPYQGDI MKKFGIRAVM
     GKGGMGQKTL QALKEHGGVY LNAIGGAAQY YADCIEAVEG VDLLEFGIPE AMWHLRVKDF
     TAVVTMDSHG NSLHEDIEKS SLEKLSQFKE PVFS