ID   3MG_ARATH               Reviewed;         254 AA.
AC   Q39147;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 141.
DE   RecName: Full=DNA-3-methyladenine glycosylase;
DE            EC=3.2.2.21;
DE   AltName: Full=3-methyladenine DNA glycosidase;
GN   Name=MAG; OrderedLocusNames=At3g12040;
GN   ORFNames=MEC18.17, T21B14.14, T21B14_115;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Aerial part;
RX   PubMed=8134381; DOI=10.1073/pnas.91.6.2240;
RA   Santerre A., Britt A.B.;
RT   "Cloning of a 3-methyladenine-DNA glycosylase from Arabidopsis thaliana.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:2240-2244(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-
CC       methyladenine, and 7-methylguanine from the damaged DNA polymer formed
CC       by alkylation lesions. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-
CC         methylguanine, 7-methylguanine and 7-methyladenine.; EC=3.2.2.21;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DNA glycosylase MPG family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X76169; CAA53763.1; -; mRNA.
DR   EMBL; AP002040; BAB03116.1; -; Genomic_DNA.
DR   EMBL; AC069473; AAG51039.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75140.1; -; Genomic_DNA.
DR   RefSeq; NP_187811.1; NM_112038.3.
DR   AlphaFoldDB; Q39147; -.
DR   SMR; Q39147; -.
DR   STRING; 3702.AT3G12040.1; -.
DR   PaxDb; Q39147; -.
DR   ProteomicsDB; 245104; -.
DR   DNASU; 820378; -.
DR   EnsemblPlants; AT3G12040.1; AT3G12040.1; AT3G12040.
DR   GeneID; 820378; -.
DR   Gramene; AT3G12040.1; AT3G12040.1; AT3G12040.
DR   KEGG; ath:AT3G12040; -.
DR   Araport; AT3G12040; -.
DR   TAIR; locus:2088639; AT3G12040.
DR   eggNOG; KOG4486; Eukaryota.
DR   HOGENOM; CLU_060471_1_0_1; -.
DR   InParanoid; Q39147; -.
DR   OMA; VEAYHHT; -.
DR   OrthoDB; 1500650at2759; -.
DR   PhylomeDB; Q39147; -.
DR   PRO; PR:Q39147; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q39147; baseline and differential.
DR   Genevisible; Q39147; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003905; F:alkylbase DNA N-glycosylase activity; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008725; F:DNA-3-methyladenine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052822; F:DNA-3-methylguanine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052821; F:DNA-7-methyladenine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043916; F:DNA-7-methylguanine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006284; P:base-excision repair; IBA:GO_Central.
DR   CDD; cd00540; AAG; 1.
DR   Gene3D; 3.10.300.10; -; 1.
DR   HAMAP; MF_00527; 3MGH; 1.
DR   InterPro; IPR011034; Formyl_transferase-like_C_sf.
DR   InterPro; IPR003180; MPG.
DR   InterPro; IPR036995; MPG_sf.
DR   PANTHER; PTHR10429; PTHR10429; 1.
DR   Pfam; PF02245; Pur_DNA_glyco; 1.
DR   SUPFAM; SSF50486; SSF50486; 1.
DR   TIGRFAMs; TIGR00567; 3mg; 1.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA repair; Hydrolase; Nucleus; Reference proteome.
FT   CHAIN           1..254
FT                   /note="DNA-3-methyladenine glycosylase"
FT                   /id="PRO_0000100068"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   254 AA;  28133 MW;  0BC880710D1BE1C5 CRC64;
     MKTPARRSKR VNQEESETNV TTRVVLRTRK TNCSKTRAAR VRPDYPLTRT TSESEMKLMP
     PEFFQIDALD LAPRLLGKFM RRDNVVLRIT EVEAYRPNDS ACHGRFGVTP RTAPVFGPGG
     HAYVYLCYGL HMMLNIVADK EGVGAAVLIR SCSPVSGMET IQERRGLKTD KPVLLNGPGK
     VGQALGLSTE WSHHPLYSPG GLELLDGGED VEKVMVGPRV GIDYALPEHV NALWRFAVAD
     TPWISAPKNT LKPL