ID   FUMD_ECO57              Reviewed;          69 AA.
AC   P0ACX7; P77274;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Fumarase D {ECO:0000250|UniProtKB:P0ACX5};
DE            EC=4.2.1.2 {ECO:0000250|UniProtKB:P0ACX5};
GN   Name=fumD {ECO:0000250|UniProtKB:P0ACX5}; Synonyms=ydhZ;
GN   OrderedLocusNames=Z2703, ECs2382;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: In vitro catalyzes the addition of water to fumarate, forming
CC       malate. Cannot catalyze the reverse reaction. Cannot use the cis-isomer
CC       maleate as substrate. {ECO:0000250|UniProtKB:P0ACX5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P0ACX5};
CC   -!- SIMILARITY: Belongs to the FumD family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG56662.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35805.1; -; Genomic_DNA.
DR   PIR; B85775; B85775.
DR   PIR; F90926; F90926.
DR   RefSeq; NP_310409.1; NC_002695.1.
DR   RefSeq; WP_000528342.1; NZ_SWKA01000004.1.
DR   AlphaFoldDB; P0ACX7; -.
DR   SMR; P0ACX7; -.
DR   STRING; 155864.EDL933_2632; -.
DR   EnsemblBacteria; AAG56662; AAG56662; Z2703.
DR   EnsemblBacteria; BAB35805; BAB35805; ECs_2382.
DR   GeneID; 66674432; -.
DR   GeneID; 914156; -.
DR   KEGG; ece:Z2703; -.
DR   KEGG; ecs:ECs_2382; -.
DR   PATRIC; fig|386585.9.peg.2495; -.
DR   HOGENOM; CLU_2755438_0_0_6; -.
DR   OMA; ALYQEMC; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR024493; FumD.
DR   Pfam; PF10965; DUF2767; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome.
FT   CHAIN           1..69
FT                   /note="Fumarase D"
FT                   /id="PRO_0000168983"
SQ   SEQUENCE   69 AA;  7907 MW;  A98BDA2AADEF5C32 CRC64;
     MGNRTKEDEL YREMCRVVGK VVLEMRDLGQ EPKHIVIAGV LRTALANKRI QRSELEKQAM
     ETVINALVK