ID   FUMD_ECOL6              Reviewed;          69 AA.
AC   P0ACX6; P77274;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Fumarase D {ECO:0000250|UniProtKB:P0ACX5};
DE            EC=4.2.1.2 {ECO:0000250|UniProtKB:P0ACX5};
GN   Name=fumD {ECO:0000250|UniProtKB:P0ACX5}; Synonyms=ydhZ;
GN   OrderedLocusNames=c2069;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: In vitro catalyzes the addition of water to fumarate, forming
CC       malate. Cannot catalyze the reverse reaction. Cannot use the cis-isomer
CC       maleate as substrate. {ECO:0000250|UniProtKB:P0ACX5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P0ACX5};
CC   -!- SIMILARITY: Belongs to the FumD family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN80529.1; -; Genomic_DNA.
DR   RefSeq; WP_000528342.1; NC_004431.1.
DR   AlphaFoldDB; P0ACX6; -.
DR   SMR; P0ACX6; -.
DR   STRING; 199310.c2069; -.
DR   EnsemblBacteria; AAN80529; AAN80529; c2069.
DR   GeneID; 66674432; -.
DR   KEGG; ecc:c2069; -.
DR   eggNOG; ENOG5032THM; Bacteria.
DR   HOGENOM; CLU_2755438_0_0_6; -.
DR   OMA; ALYQEMC; -.
DR   BioCyc; ECOL199310:C2069-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR024493; FumD.
DR   Pfam; PF10965; DUF2767; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..69
FT                   /note="Fumarase D"
FT                   /id="PRO_0000168984"
SQ   SEQUENCE   69 AA;  7907 MW;  A98BDA2AADEF5C32 CRC64;
     MGNRTKEDEL YREMCRVVGK VVLEMRDLGQ EPKHIVIAGV LRTALANKRI QRSELEKQAM
     ETVINALVK