FUMD_ECOLI
ID FUMD_ECOLI Reviewed; 69 AA.
AC P0ACX5; P77274; Q2MB57;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Fumarase D {ECO:0000303|PubMed:27941785};
DE EC=4.2.1.2 {ECO:0000269|PubMed:27941785};
GN Name=fumD {ECO:0000303|PubMed:27941785};
GN Synonyms=ydhZ {ECO:0000312|EMBL:AAC74745.1};
GN OrderedLocusNames=b1675, JW1665;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9023191; DOI=10.1128/jb.179.4.1105-1111.1997;
RA Hensel M., Shea J.E., Baeumler A.J., Gleeson C., Blattner F.R.,
RA Holden D.W.;
RT "Analysis of the boundaries of Salmonella pathogenicity island 2 and the
RT corresponding chromosomal region of Escherichia coli K-12.";
RL J. Bacteriol. 179:1105-1111(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12 / BW25113;
RX PubMed=25316676; DOI=10.1093/bioinformatics/btu671;
RA Vlasblom J., Zuberi K., Rodriguez H., Arnold R., Gagarinova A., Deineko V.,
RA Kumar A., Leung E., Rizzolo K., Samanfar B., Chang L., Phanse S.,
RA Golshani A., Greenblatt J.F., Houry W.A., Emili A., Morris Q., Bader G.,
RA Babu M.;
RT "Novel function discovery with GeneMANIA: a new integrated resource for
RT gene function prediction in Escherichia coli.";
RL Bioinformatics 31:306-310(2015).
RN [5]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=K12;
RX PubMed=27941785; DOI=10.1038/nmeth.4103;
RA Sevin D.C., Fuhrer T., Zamboni N., Sauer U.;
RT "Nontargeted in vitro metabolomics for high-throughput identification of
RT novel enzymes in Escherichia coli.";
RL Nat. Methods 14:187-194(2017).
CC -!- FUNCTION: In vitro catalyzes the addition of water to fumarate, forming
CC malate. Cannot catalyze the reverse reaction. Cannot use the cis-isomer
CC maleate as substrate. {ECO:0000269|PubMed:27941785}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC Evidence={ECO:0000269|PubMed:27941785};
CC -!- DISRUPTION PHENOTYPE: Cells grow to slightly higher density than wild-
CC type in sublethal levels of streptomycin (PubMed:25316676).
CC {ECO:0000269|PubMed:25316676}.
CC -!- SIMILARITY: Belongs to the FumD family. {ECO:0000305}.
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DR EMBL; U68703; AAB47951.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74745.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76499.1; -; Genomic_DNA.
DR PIR; C64925; C64925.
DR RefSeq; NP_416190.1; NC_000913.3.
DR RefSeq; WP_000528342.1; NZ_STEB01000003.1.
DR AlphaFoldDB; P0ACX5; -.
DR SMR; P0ACX5; -.
DR BioGRID; 4259670; 4.
DR IntAct; P0ACX5; 10.
DR STRING; 511145.b1675; -.
DR jPOST; P0ACX5; -.
DR PaxDb; P0ACX5; -.
DR PRIDE; P0ACX5; -.
DR EnsemblBacteria; AAC74745; AAC74745; b1675.
DR EnsemblBacteria; BAE76499; BAE76499; BAE76499.
DR GeneID; 66674432; -.
DR GeneID; 946180; -.
DR KEGG; ecj:JW1665; -.
DR KEGG; eco:b1675; -.
DR PATRIC; fig|511145.12.peg.1746; -.
DR EchoBASE; EB3718; -.
DR eggNOG; ENOG5032THM; Bacteria.
DR HOGENOM; CLU_2755438_0_0_6; -.
DR OMA; ALYQEMC; -.
DR PhylomeDB; P0ACX5; -.
DR BioCyc; EcoCyc:G6903-MON; -.
DR BioCyc; MetaCyc:G6903-MON; -.
DR PRO; PR:P0ACX5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004333; F:fumarate hydratase activity; IDA:EcoliWiki.
DR InterPro; IPR024493; FumD.
DR Pfam; PF10965; DUF2767; 1.
PE 1: Evidence at protein level;
KW Lyase; Reference proteome.
FT CHAIN 1..69
FT /note="Fumarase D"
FT /id="PRO_0000168982"
SQ SEQUENCE 69 AA; 7907 MW; A98BDA2AADEF5C32 CRC64;
MGNRTKEDEL YREMCRVVGK VVLEMRDLGQ EPKHIVIAGV LRTALANKRI QRSELEKQAM
ETVINALVK
