FUME_ECOLI
ID FUME_ECOLI Reviewed; 169 AA.
AC P11663; Q2M9R3;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Fumarase E {ECO:0000303|PubMed:27941785};
DE EC=4.2.1.2 {ECO:0000269|PubMed:27941785};
GN Name=fumE {ECO:0000303|PubMed:27941785};
GN Synonyms=yggD {ECO:0000312|EMBL:AAC75966.1};
GN OrderedLocusNames=b2929, JW2896;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-155.
RC STRAIN=K12 / CS520;
RX PubMed=2546007; DOI=10.1111/j.1365-2958.1989.tb00221.x;
RA Alefounder P.R., Perham R.N.;
RT "Identification, molecular cloning and sequence analysis of a gene cluster
RT encoding the class II fructose 1,6-bisphosphate aldolase, 3-
RT phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate
RT dehydrogenase of Escherichia coli.";
RL Mol. Microbiol. 3:723-732(1989).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=K12;
RX PubMed=27941785; DOI=10.1038/nmeth.4103;
RA Sevin D.C., Fuhrer T., Zamboni N., Sauer U.;
RT "Nontargeted in vitro metabolomics for high-throughput identification of
RT novel enzymes in Escherichia coli.";
RL Nat. Methods 14:187-194(2017).
CC -!- FUNCTION: In vitro catalyzes the addition of water to fumarate, forming
CC malate. Cannot catalyze the reverse reaction. Cannot use the cis-isomer
CC maleate as substrate. {ECO:0000269|PubMed:27941785}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC Evidence={ECO:0000269|PubMed:27941785};
CC -!- SIMILARITY: Belongs to the MtlR/FumE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA32601.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U28377; AAA69096.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75966.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76993.1; -; Genomic_DNA.
DR EMBL; X14436; CAA32601.1; ALT_INIT; Genomic_DNA.
DR PIR; H65077; QQEC2B.
DR RefSeq; NP_417404.1; NC_000913.3.
DR RefSeq; WP_000224147.1; NZ_SSZK01000003.1.
DR AlphaFoldDB; P11663; -.
DR SMR; P11663; -.
DR BioGRID; 4262333; 86.
DR IntAct; P11663; 5.
DR STRING; 511145.b2929; -.
DR PaxDb; P11663; -.
DR PRIDE; P11663; -.
DR EnsemblBacteria; AAC75966; AAC75966; b2929.
DR EnsemblBacteria; BAE76993; BAE76993; BAE76993.
DR GeneID; 946861; -.
DR KEGG; ecj:JW2896; -.
DR KEGG; eco:b2929; -.
DR PATRIC; fig|1411691.4.peg.3803; -.
DR EchoBASE; EB1151; -.
DR eggNOG; COG3722; Bacteria.
DR HOGENOM; CLU_132701_0_0_6; -.
DR OMA; VSKLMFA; -.
DR PhylomeDB; P11663; -.
DR BioCyc; EcoCyc:EG11162-MON; -.
DR BioCyc; MetaCyc:EG11162-MON; -.
DR EvolutionaryTrace; P11663; -.
DR PRO; PR:P11663; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004333; F:fumarate hydratase activity; IDA:EcoCyc.
DR InterPro; IPR007761; MtlR-like.
DR InterPro; IPR038026; MtlR-like_sf.
DR PANTHER; PTHR37941; PTHR37941; 1.
DR Pfam; PF05068; MtlR; 1.
DR SUPFAM; SSF158668; SSF158668; 1.
PE 1: Evidence at protein level;
KW Lyase; Reference proteome.
FT CHAIN 1..169
FT /note="Fumarase E"
FT /id="PRO_0000169363"
SQ SEQUENCE 169 AA; 19272 MW; 427302F600960D31 CRC64;
MATLTEDDVL EQLDAQDNLF SFMKTAHTIL LQGIRQFLPS LFVDNDEEIV EYAVKPLLAQ
SGPLDDIDVA LRLIYALGKM DKWLYADITH FSQFWHYLNE QDETPGFADD MTWDFISNVN
SITRNAMLYD ALKAMKFADF SVWSEARFSG MVKTALTLAV TTTLKELTP
