FUME_SHIFL
ID FUME_SHIFL Reviewed; 169 AA.
AC Q83Q96; Q7C035;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Fumarase E {ECO:0000250|UniProtKB:P11663};
DE EC=4.2.1.2 {ECO:0000250|UniProtKB:P11663};
GN Name=fumE {ECO:0000250|UniProtKB:P11663};
GN Synonyms=yggD {ECO:0000303|PubMed:19840941, ECO:0000312|EMBL:AAP18223.1};
GN OrderedLocusNames=S3120 {ECO:0000312|EMBL:AAP18223.1},
GN SF2919 {ECO:0000312|EMBL:AAN44400.1};
GN ORFNames=AJR24_04990 {ECO:0000312|EMBL:OLM67743.1};
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BCW_4876;
RA Weis A.M., Gilpin B., Weimer B.C.;
RT "Shigella draft genomes.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), AND SUBUNIT.
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=19840941; DOI=10.1074/jbc.m109.062679;
RA Tan K., Clancy S., Borovilos M., Zhou M., Hoerer S., Moy S., Volkart L.L.,
RA Sassoon J., Baumann U., Joachimiak A.;
RT "The mannitol operon repressor MtlR belongs to a new class of transcription
RT regulators in bacteria.";
RL J. Biol. Chem. 284:36670-36679(2009).
CC -!- FUNCTION: In vitro catalyzes the addition of water to fumarate, forming
CC malate. Cannot catalyze the reverse reaction. Cannot use the cis-isomer
CC maleate as substrate. {ECO:0000250|UniProtKB:P11663}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC Evidence={ECO:0000250|UniProtKB:P11663};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19840941}.
CC -!- SIMILARITY: Belongs to the MtlR/FumE family. {ECO:0000305}.
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DR EMBL; AE005674; AAN44400.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP18223.1; -; Genomic_DNA.
DR EMBL; MSJZ01000073; OLM67743.1; -; Genomic_DNA.
DR RefSeq; NP_708693.1; NC_004337.2.
DR RefSeq; WP_000224135.1; NZ_WPGW01000018.1.
DR PDB; 3C8G; X-ray; 2.50 A; A/C/D=1-169.
DR PDBsum; 3C8G; -.
DR AlphaFoldDB; Q83Q96; -.
DR SMR; Q83Q96; -.
DR STRING; 198214.SF2919; -.
DR EnsemblBacteria; AAN44400; AAN44400; SF2919.
DR EnsemblBacteria; AAP18223; AAP18223; S3120.
DR GeneID; 1026023; -.
DR KEGG; sfl:SF2919; -.
DR KEGG; sfx:S3120; -.
DR PATRIC; fig|198214.7.peg.3473; -.
DR HOGENOM; CLU_132701_0_0_6; -.
DR OMA; VSKLMFA; -.
DR OrthoDB; 1375907at2; -.
DR EvolutionaryTrace; Q83Q96; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-EC.
DR InterPro; IPR007761; MtlR-like.
DR InterPro; IPR038026; MtlR-like_sf.
DR PANTHER; PTHR37941; PTHR37941; 1.
DR Pfam; PF05068; MtlR; 1.
DR SUPFAM; SSF158668; SSF158668; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Reference proteome.
FT CHAIN 1..169
FT /note="Fumarase E"
FT /id="PRO_0000439649"
FT HELIX 6..15
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 19..23
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 26..37
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 38..41
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 47..52
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 67..76
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 84..100
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 110..117
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 120..123
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 126..132
FT /evidence="ECO:0007829|PDB:3C8G"
FT HELIX 145..166
FT /evidence="ECO:0007829|PDB:3C8G"
SQ SEQUENCE 169 AA; 19210 MW; 0EF3182094AEF8DC CRC64;
MATLTEDDVL EQLDAQDNLF SFMKTAHSIL LQGIRQFLPS LFVDNDEEIV EYAVKPLLAQ
SGPLDDIDVA LRLIYALGKM DKWLYADITH FSQYWHYLNE QDETPGFADD ITWDFISNVN
SITRNATLYD ALKAMKFADF AVWSEARFSG MVKTALTLAV TTTLKELTP
