ID   3MG_ENCCU               Reviewed;         208 AA.
AC   Q8SQI1;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Probable DNA-3-methyladenine glycosylase;
DE            EC=3.2.2.21;
DE   AltName: Full=3-methyladenine DNA glycosidase;
GN   OrderedLocusNames=ECU05_1590;
GN   and
GN   OrderedLocusNames=ECU11_0140;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-
CC       methyladenine, and 7-methylguanine from the damaged DNA polymer formed
CC       by alkylation lesions. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-
CC         methylguanine, 7-methylguanine and 7-methyladenine.; EC=3.2.2.21;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: Belongs to the DNA glycosylase MPG family. {ECO:0000305}.
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DR   EMBL; AL590445; CAD26679.1; -; Genomic_DNA.
DR   EMBL; AL590450; CAD25924.1; -; Genomic_DNA.
DR   RefSeq; NP_586320.1; NM_001042153.1.
DR   RefSeq; NP_597502.1; NM_001041368.1.
DR   AlphaFoldDB; Q8SQI1; -.
DR   SMR; Q8SQI1; -.
DR   STRING; 284813.Q8SQI1; -.
DR   GeneID; 859169; -.
DR   GeneID; 859971; -.
DR   KEGG; ecu:ECU05_1590; -.
DR   KEGG; ecu:ECU11_0140; -.
DR   VEuPathDB; MicrosporidiaDB:ECU05_1590; -.
DR   VEuPathDB; MicrosporidiaDB:ECU11_0140; -.
DR   HOGENOM; CLU_060471_2_1_1; -.
DR   InParanoid; Q8SQI1; -.
DR   OrthoDB; 1500650at2759; -.
DR   Proteomes; UP000000819; Chromosome V.
DR   Proteomes; UP000000819; Chromosome XI.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008725; F:DNA-3-methyladenine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052822; F:DNA-3-methylguanine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052821; F:DNA-7-methyladenine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043916; F:DNA-7-methylguanine glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR   CDD; cd00540; AAG; 1.
DR   Gene3D; 3.10.300.10; -; 1.
DR   HAMAP; MF_00527; 3MGH; 1.
DR   InterPro; IPR011034; Formyl_transferase-like_C_sf.
DR   InterPro; IPR003180; MPG.
DR   InterPro; IPR036995; MPG_sf.
DR   PANTHER; PTHR10429; PTHR10429; 1.
DR   Pfam; PF02245; Pur_DNA_glyco; 1.
DR   SUPFAM; SSF50486; SSF50486; 1.
DR   TIGRFAMs; TIGR00567; 3mg; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Hydrolase; Nucleus; Reference proteome.
FT   CHAIN           1..208
FT                   /note="Probable DNA-3-methyladenine glycosylase"
FT                   /id="PRO_0000100069"
SQ   SEQUENCE   208 AA;  23773 MW;  A4A22F1DAD5B443F CRC64;
     MDMGIPCSQL ARRLLGKMLC RRIEGRTTKG MIVETEAYLG KEDKACHSYG GRRTERNSAM
     YMKAGTCYVY RIYGRYECFN ISSVEAGAGV LVRALEPLCG VSEMRERRGG RVKDRDIANG
     PSKLCIAMGI TRREIDKEWI AGSEKIWLEE GREVADPEIV AGRRIGIRNC GEWEEKKLRF
     YIRDNEFVSC IRRRELGNRK HGSVQQLP