FUR4_YEAST
ID FUR4_YEAST Reviewed; 633 AA.
AC P05316; D6VQ23;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Uracil permease;
GN Name=FUR4; OrderedLocusNames=YBR021W; ORFNames=YBR0303;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX PubMed=3276521; DOI=10.1111/j.1432-1033.1988.tb13806.x;
RA Jund R., Weber E., Chevallier M.-R.;
RT "Primary structure of the uracil transport protein of Saccharomyces
RT cerevisiae.";
RL Eur. J. Biochem. 171:417-424(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091864; DOI=10.1002/yea.320100010;
RA Smits P.H.M., de Haan M., Maat C., Grivell L.A.;
RT "The complete sequence of a 33 kb fragment on the right arm of chromosome
RT II from Saccharomyces cerevisiae reveals 16 open reading frames, including
RT ten new open reading frames, five previously identified genes and a
RT homologue of the SCO1 gene.";
RL Yeast 10:S75-S80(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Transport of uracil.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- PTM: Glycosylated (possible); but there is not yet direct biochemical
CC evidence for it.
CC -!- SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family.
CC {ECO:0000305}.
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DR EMBL; X76078; CAA53678.1; -; Genomic_DNA.
DR EMBL; Z35890; CAA84963.1; -; Genomic_DNA.
DR EMBL; X06830; CAA29986.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07143.1; -; Genomic_DNA.
DR PIR; S45877; S45877.
DR RefSeq; NP_009577.1; NM_001178369.1.
DR AlphaFoldDB; P05316; -.
DR SMR; P05316; -.
DR BioGRID; 32724; 21.
DR DIP; DIP-4892N; -.
DR IntAct; P05316; 32.
DR STRING; 4932.YBR021W; -.
DR TCDB; 2.A.39.3.2; the nucleobase:cation symporter-1 (ncs1) family.
DR iPTMnet; P05316; -.
DR MaxQB; P05316; -.
DR PaxDb; P05316; -.
DR PRIDE; P05316; -.
DR EnsemblFungi; YBR021W_mRNA; YBR021W; YBR021W.
DR GeneID; 852309; -.
DR KEGG; sce:YBR021W; -.
DR SGD; S000000225; FUR4.
DR VEuPathDB; FungiDB:YBR021W; -.
DR eggNOG; KOG2466; Eukaryota.
DR GeneTree; ENSGT00940000176299; -.
DR HOGENOM; CLU_021555_2_2_1; -.
DR InParanoid; P05316; -.
DR OMA; CGTDMSA; -.
DR BioCyc; YEAST:G3O-29002-MON; -.
DR PRO; PR:P05316; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P05316; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0032126; C:eisosome; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015205; F:nucleobase transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015505; F:uracil:cation symporter activity; IDA:SGD.
DR GO; GO:0015851; P:nucleobase transport; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IDA:SGD.
DR GO; GO:0015857; P:uracil transport; IDA:SGD.
DR InterPro; IPR012681; NCS1.
DR InterPro; IPR001248; Pur-cyt_permease.
DR InterPro; IPR045225; Uracil/uridine/allantoin_perm.
DR PANTHER; PTHR30618; PTHR30618; 1.
DR Pfam; PF02133; Transp_cyt_pur; 1.
DR TIGRFAMs; TIGR00800; ncs1; 1.
PE 3: Inferred from homology;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..633
FT /note="Uracil permease"
FT /id="PRO_0000197925"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 217
FT /note="M -> I (in Ref. 1; CAA29986)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="K -> R (in Ref. 1; CAA29986)"
FT /evidence="ECO:0000305"
FT CONFLICT 320
FT /note="G -> R (in Ref. 1; CAA29986)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 633 AA; 71736 MW; 2FFCD9529412B2D6 CRC64;
MPDNLSLHLS GSSKRLNSRQ LMESSNETFA PNNVDLEKEY KSSQSNITTE VYEASSFEEK
VSSEKPQYSS FWKKIYYEYV VVDKSILGVS ILDSFMYNQD LKPVEKERRV WSWYNYCYFW
LAECFNINTW QIAATGLQLG LNWWQCWITI WIGYGFVGAF VVLASRVGSA YHLSFPISSR
ASFGIFFSLW PVINRVVMAI VWYSVQAYIA ATPVSLMLKS IFGKDLQDKI PDHFGSPNAT
TYEFMCFFIF WAASLPFLLV PPHKIRHLFT VKAVLVPFAS FGFLIWAIRR AHGRIALGSL
TDVQPHGSAF SWAFLRSLMG CMANFSTMVI NAPDFSRFSK NPNSALWSQL VCIPFLFSIT
CLIGILVTAA GYEIYGINYW SPLDVLEKFL QTTYNKGTRA GVFLISFVFA VAQLGTNISA
NSLSCGTDMS AIFPKFINIK RGSLFCAAMA LCICPWNLMA TSSKFTMALS AYAIFLSSIA
GVVCSDYFVV RRGYIKLTHI YSHQKGSFYM YGNRFGINWR ALAAYLCGVA PCLPGFIAEV
GAPAIKVSDG AMKLYYLSYW VGYGLSFSSY TALCYFFPVP GCPVNNIIKD KGWFQRWANV
DDFEEEWKDT IERDDLVDDN ISVYEHEHEK TFI
