ID   FUR_KLEPN               Reviewed;         155 AA.
AC   P45599; O07027;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Ferric uptake regulation protein;
DE            Short=Ferric uptake regulator;
GN   Name=fur;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 / NCTC
RC   9633;
RX   PubMed=9055816; DOI=10.1016/s0378-1119(96)00642-7;
RA   Achenbach L.A., Yang W.;
RT   "The fur gene from Klebsiella pneumoniae: characterization, genomic
RT   organization and phylogenetic analysis.";
RL   Gene 185:201-207(1997).
CC   -!- FUNCTION: Acts as a global negative controlling element, employing
CC       Fe(2+) as a cofactor to bind the operator of the repressed genes.
CC       Regulates the expression of several outer-membrane proteins including
CC       the iron transport operon (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Fur family. {ECO:0000305}.
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DR   EMBL; L23871; AAB51077.1; -; Genomic_DNA.
DR   PDB; 5L0P; X-ray; 2.30 A; A=3-83.
DR   PDBsum; 5L0P; -.
DR   AlphaFoldDB; P45599; -.
DR   BMRB; P45599; -.
DR   SMR; P45599; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd07153; Fur_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.1490.190; -; 1.
DR   InterPro; IPR002481; FUR.
DR   InterPro; IPR043135; Fur_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33202; PTHR33202; 1.
DR   Pfam; PF01475; FUR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; Iron; Metal-binding; Repressor;
KW   Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..155
FT                   /note="Ferric uptake regulation protein"
FT                   /id="PRO_0000095557"
FT   REGION          1..84
FT                   /note="DNA-binding"
FT                   /evidence="ECO:0000250"
FT   REGION          85..155
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000250"
FT   BINDING         33
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         81
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         87
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         89
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         90
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         93
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         96
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         101
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         108
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         125
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   HELIX           4..10
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   HELIX           17..26
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   STRAND          32..35
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   HELIX           36..45
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   HELIX           52..64
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   STRAND          67..71
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   TURN            74..76
FT                   /evidence="ECO:0007829|PDB:5L0P"
FT   STRAND          79..82
FT                   /evidence="ECO:0007829|PDB:5L0P"
SQ   SEQUENCE   155 AA;  17779 MW;  C83D34650B68F5BD CRC64;
     MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN
     QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIELR QREIASRHGI
     RLTNHSLYLY GHCAEGDCRE TNTPTTRWKN NSPFR