ID   FUR_VIBVU               Reviewed;         149 AA.
AC   P33117;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Ferric uptake regulation protein;
DE            Short=Ferric uptake regulator;
GN   Name=fur; OrderedLocusNames=VV1_0175;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7678593; DOI=10.1128/jb.175.3.706-715.1993;
RA   Litwin C.M., Calderwood S.B.;
RT   "Cloning and genetic analysis of the Vibrio vulnificus fur gene and
RT   construction of a fur mutant by in vivo marker exchange.";
RL   J. Bacteriol. 175:706-715(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Fur acts as a repressor, employing Fe(2+) as a cofactor to
CC       bind the operator of the iron transport operon.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Fur family. {ECO:0000305}.
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DR   EMBL; L06428; AAA27521.1; -; Genomic_DNA.
DR   EMBL; AE016795; AAO08713.1; -; Genomic_DNA.
DR   PIR; A47054; A47054.
DR   RefSeq; WP_011078292.1; NC_004459.3.
DR   AlphaFoldDB; P33117; -.
DR   SMR; P33117; -.
DR   EnsemblBacteria; AAO08713; AAO08713; VV1_0175.
DR   KEGG; vvu:VV1_0175; -.
DR   HOGENOM; CLU_096072_3_3_6; -.
DR   OMA; HDHVILT; -.
DR   Proteomes; UP000002275; Chromosome 1.
DR   CollecTF; EXPREG_00000a30; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR   GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:CollecTF.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IPI:CollecTF.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:CollecTF.
DR   CDD; cd07153; Fur_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.1490.190; -; 1.
DR   InterPro; IPR002481; FUR.
DR   InterPro; IPR043135; Fur_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33202; PTHR33202; 1.
DR   Pfam; PF01475; FUR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Iron; Metal-binding; Repressor; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..149
FT                   /note="Ferric uptake regulation protein"
FT                   /id="PRO_0000095587"
FT   REGION          1..84
FT                   /note="DNA-binding"
FT                   /evidence="ECO:0000250"
FT   REGION          85..142
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000250"
FT   BINDING         33
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         81
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         87
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         89
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         90
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         93
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         96
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         101
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         108
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         125
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        149
FT                   /note="N -> S (in Ref. 1; AAA27521)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   149 AA;  16743 MW;  3C54EBB82C0F21C8 CRC64;
     MSDNNQALKD AGLKVTLPRL KILEVLQQPD CQHISAEDLY KKLIDLGEEI GLATVYRVLN
     QFDDAGIVTR HHFEGGKSVF ELSTQHHHDH LVCLDCGEVI EFSDDIIEER QKEIAAAYNV
     QLTNHSLYLY GKCGDGSCKG NPDAHKRKN