FUS2_YEAST
ID FUS2_YEAST Reviewed; 677 AA.
AC Q05670; D6W058; Q05023;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Nuclear fusion protein FUS2;
GN Name=FUS2; OrderedLocusNames=YMR232W; ORFNames=YM9959.14;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7559752; DOI=10.1083/jcb.130.6.1283;
RA Elion E.A., Trueheart J., Fink G.R.;
RT "Fus2 localizes near the site of cell fusion and is required for both cell
RT fusion and nuclear alignment during zygote formation.";
RL J. Cell Biol. 130:1283-1296(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-67; SER-72; SER-84;
RP THR-88; SER-100 AND SER-106, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE
RP SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
CC -!- FUNCTION: Promotes cell fusion during zygote formation.
CC -!- SUBCELLULAR LOCATION: Cell tip. Note=Localizes at the mating projection
CC tip (shmoo).
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DR EMBL; X90752; CAA62275.1; -; Genomic_DNA.
DR EMBL; Z49939; CAA90203.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10132.1; -; Genomic_DNA.
DR PIR; S57599; S57599.
DR RefSeq; NP_013959.1; NM_001182739.1.
DR AlphaFoldDB; Q05670; -.
DR BioGRID; 35410; 81.
DR DIP; DIP-2711N; -.
DR IntAct; Q05670; 12.
DR MINT; Q05670; -.
DR STRING; 4932.YMR232W; -.
DR iPTMnet; Q05670; -.
DR PaxDb; Q05670; -.
DR PRIDE; Q05670; -.
DR EnsemblFungi; YMR232W_mRNA; YMR232W; YMR232W.
DR GeneID; 855272; -.
DR KEGG; sce:YMR232W; -.
DR SGD; S000004845; FUS2.
DR VEuPathDB; FungiDB:YMR232W; -.
DR eggNOG; ENOG502RC15; Eukaryota.
DR HOGENOM; CLU_392821_0_0_1; -.
DR InParanoid; Q05670; -.
DR OMA; YKNDYFH; -.
DR BioCyc; YEAST:G3O-32913-MON; -.
DR Reactome; R-SCE-416482; G alpha (12/13) signalling events.
DR Reactome; R-SCE-9013148; CDC42 GTPase cycle.
DR PRO; PR:Q05670; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q05670; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0000747; P:conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0000755; P:cytogamy; IMP:SGD.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0031385; P:regulation of termination of mating projection growth; IMP:SGD.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.20.900.10; -; 1.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR PROSITE; PS50010; DH_2; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..677
FT /note="Nuclear fusion protein FUS2"
FT /id="PRO_0000080954"
FT DOMAIN 112..326
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT MOD_RES 20
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 72
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 84
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 88
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 100
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 106
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CONFLICT 185
FT /note="L -> R (in Ref. 1; CAA62275)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="F -> L (in Ref. 1; CAA62275)"
FT /evidence="ECO:0000305"
FT CONFLICT 543..546
FT /note="Missing (in Ref. 1; CAA62275)"
FT /evidence="ECO:0000305"
FT CONFLICT 589..677
FT /note="HDTIECILLNYIKVFLKYLEIIAGGKKYLQKDLENMSLNDSIATGQIKNLDI
FT LQCYSKSRYMTKRMVRKDWPFPGDPSGSRVVRKLFEL -> PRYYRMYLVELYQSLLKI
FT FGNHCWWKKIPAKRS (in Ref. 1; CAA62275)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 677 AA; 79037 MW; D7C89ED0738EA31F CRC64;
MFKTSYNLYD LNYPKNDSLT PIRDYKNDYF HKNDDKLPEI VRKPTRKLSK HENKLNDKKF
TNKRPASLDL HSIVESLSNK KIYSPINTEI FQNVVRLNLS PQIPNSPHEG CKFYKIVQEF
YLSEVEYYNN LLTANNVYRK ALNSDPRFKN KLVKLDSSDE LLLFGNIDTI ASISKILVTA
IKDLLLAKQR GKMLDANEWQ KIFTKNEVQQ QLYSTFDISE AFEQHLLRIK STYTSYFVSH
QKQMELFTTL RMNKNHFFNK WYEYCLKESG CIKLEDILKS PMKRLTQWID TLETLESCYE
DILSPELGLK LSPTRRKYSL FSNKLETEVS EYKSNSMYNF SLTPSEIIQS YDEDQFTHLL
KPPDKQNKNI CNASRQESNL DNSRVPSLLS GSSSYYSDVS GLEIVTNTST ASAEMINLKM
DEETEFFTLA DHISKFKKVM KGLLELKKNL LKNDLSGIID ISLRRINAWK KVIECERPSG
AFFAHDNLIS TMCSSYIDKL HEQKNQVTIL KLTELETDVM NPLERIIAHC TTVKSKLKDL
QALKKDYMLF LQEKKANVRD IKRDLLGMHF QNLQNQMKRE LPVFITLIHD TIECILLNYI
KVFLKYLEII AGGKKYLQKD LENMSLNDSI ATGQIKNLDI LQCYSKSRYM TKRMVRKDWP
FPGDPSGSRV VRKLFEL
