FUS3_ARATH
ID FUS3_ARATH Reviewed; 313 AA.
AC Q9LW31; O82411; Q700C8; Q9SAT8;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=B3 domain-containing transcription factor FUS3;
DE AltName: Full=Protein FUSCA3;
GN Name=FUS3; OrderedLocusNames=At3g26790; ORFNames=MDJ14.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia; TISSUE=Silique;
RX PubMed=9807814; DOI=10.1046/j.1365-313x.1998.00259.x;
RA Luerssen H., Kirik V., Herrmann P., Misera S.;
RT "FUSCA3 encodes a protein with a conserved VP1/AB13-like B3 domain which is
RT of functional importance for the regulation of seed maturation in
RT Arabidopsis thaliana.";
RL Plant J. 15:755-764(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=12244252; DOI=10.2307/3869865;
RA Keith K., Kraml M., Dengler N.G., McCourt P.;
RT "fusca3: a heterochronic mutation affecting late embryo development in
RT Arabidopsis.";
RL Plant Cell 6:589-600(1994).
RN [6]
RP FUNCTION.
RX PubMed=15363412; DOI=10.1016/j.devcel.2004.06.017;
RA Gazzarrini S., Tsuchiya Y., Lumba S., Okamoto M., McCourt P.;
RT "The transcription factor FUSCA3 controls developmental timing in
RT Arabidopsis through the hormones gibberellin and abscisic acid.";
RL Dev. Cell 7:373-385(2004).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=14675433; DOI=10.1046/j.1365-313x.2003.01939.x;
RA Tsuchiya Y., Nambara E., Naito S., McCourt P.;
RT "The FUS3 transcription factor functions through the epidermal regulator
RT TTG1 during embryogenesis in Arabidopsis.";
RL Plant J. 37:73-81(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
RN [9]
RP INTERACTION WITH KIN10, PHOSPHORYLATION, FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=22026387; DOI=10.1111/j.1365-313x.2011.04832.x;
RA Tsai A.Y., Gazzarrini S.;
RT "AKIN10 and FUSCA3 interact to control lateral organ development and phase
RT transitions in Arabidopsis.";
RL Plant J. 69:809-821(2012).
RN [10]
RP FUNCTION.
RX PubMed=22902692; DOI=10.4161/psb.21549;
RA Tsai A.Y., Gazzarrini S.;
RT "Overlapping and distinct roles of AKIN10 and FUSCA3 in ABA and sugar
RT signaling during seed germination.";
RL Plant Signal. Behav. 7:1238-1242(2012).
RN [11]
RP MUTAGENESIS OF SER-55; SER-56 AND SER-57, AND ACTIVITY REGULATION.
RX PubMed=28922765; DOI=10.1093/jxb/erx233;
RA Chan A., Carianopol C., Tsai A.Y., Varathanajah K., Chiu R.S.,
RA Gazzarrini S.;
RT "SnRK1 phosphorylation of FUSCA3 positively regulates embryogenesis, seed
RT yield, and plant growth at high temperature in Arabidopsis.";
RL J. Exp. Bot. 68:4219-4231(2017).
RN [12]
RP ERRATUM OF PUBMED:28922765.
RX PubMed=29140438; DOI=10.1093/jxb/erx379;
RA Chan A., Carianopol C., Tsai A.Y., Varatharajah K., Chiu R.S.,
RA Gazzarrini S.;
RT "Corrigendum: SnRK1 phosphorylation of FUSCA3 positively regulates
RT embryogenesis, seed yield, and plant growth at high temperature in
RT Arabidopsis.";
RL J. Exp. Bot. 68:5981-5981(2017).
CC -!- FUNCTION: Transcription regulator involved in gene regulation during
CC late embryogenesis. Its expression to the epidermis is sufficient to
CC control foliar organ identity by regulating positively the synthesis
CC abscisic acid (ABA) and negatively gibberellin production. Negatively
CC regulates TTG1 in the embryo. Positively regulates the abundance of the
CC ABI3 protein in the seed. Cooperates with KIN10 to regulate
CC developmental phase transitions and lateral organ development and act
CC both as positive regulators of abscisic acid (ABA) signaling during
CC germination (PubMed:22026387, PubMed:22902692).
CC {ECO:0000269|PubMed:14675433, ECO:0000269|PubMed:15363412,
CC ECO:0000269|PubMed:22026387, ECO:0000269|PubMed:22902692}.
CC -!- ACTIVITY REGULATION: Phosphorylation by KIN10 is required to positively
CC regulates embryogenesis, seed yield, and plant growth at high
CC temperature. {ECO:0000269|PubMed:28922765}.
CC -!- SUBUNIT: Interacts with KIN10. {ECO:0000269|PubMed:22026387}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons and hypocotyls.
CC {ECO:0000269|PubMed:22026387}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing embryo. At globular stage,
CC expressed in all cells of the embryo proper. At heart stage,
CC preferentially expressed in the protodermal tissue. In mature embryo,
CC expressed in the provascular tissue, root cap and mature epidermis.
CC Expressed in the aleurone layer in mature seed. Expressed in leaf
CC primordia and shoot apical meristem (PubMed:22026387).
CC {ECO:0000269|PubMed:14675433, ECO:0000269|PubMed:22026387,
CC ECO:0000269|PubMed:9807814}.
CC -!- PTM: Phosphorylation by KIN10 increases its stability. Phosphorylated
CC at one or more of the Ser-55, Ser-56 and/or Ser-57 residues.
CC {ECO:0000269|PubMed:22026387}.
CC -!- DISRUPTION PHENOTYPE: Accumulation of anthocyanins in embryo. Presence
CC of trichomes on cotyledons. Unusual pattern of storage product
CC accumulation in embryos and cotyledons. {ECO:0000269|PubMed:12244252}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC35246.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAC35247.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB01226.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF016264; AAC35246.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF016265; AAC35247.1; ALT_INIT; mRNA.
DR EMBL; AB016889; BAB01226.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77216.1; -; Genomic_DNA.
DR EMBL; AJ630497; CAG25870.1; -; mRNA.
DR EMBL; AY568669; AAS79559.1; -; mRNA.
DR RefSeq; NP_566799.1; NM_113591.4.
DR PDB; 6J9B; X-ray; 1.90 A; A/D=89-201.
DR PDBsum; 6J9B; -.
DR AlphaFoldDB; Q9LW31; -.
DR SMR; Q9LW31; -.
DR BioGRID; 7623; 2.
DR STRING; 3702.AT3G26790.1; -.
DR PaxDb; Q9LW31; -.
DR PRIDE; Q9LW31; -.
DR ProteomicsDB; 230553; -.
DR EnsemblPlants; AT3G26790.1; AT3G26790.1; AT3G26790.
DR GeneID; 822293; -.
DR Gramene; AT3G26790.1; AT3G26790.1; AT3G26790.
DR KEGG; ath:AT3G26790; -.
DR Araport; AT3G26790; -.
DR TAIR; locus:2088439; AT3G26790.
DR eggNOG; ENOG502QPW6; Eukaryota.
DR HOGENOM; CLU_084617_0_0_1; -.
DR InParanoid; Q9LW31; -.
DR OMA; IPIRMED; -.
DR OrthoDB; 317013at2759; -.
DR PhylomeDB; Q9LW31; -.
DR PRO; PR:Q9LW31; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LW31; baseline and differential.
DR Genevisible; Q9LW31; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0019900; F:kinase binding; IPI:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
DR GO; GO:0010373; P:negative regulation of gibberellin biosynthetic process; IMP:UniProtKB.
DR GO; GO:0099402; P:plant organ development; IMP:UniProtKB.
DR GO; GO:0010116; P:positive regulation of abscisic acid biosynthetic process; IMP:TAIR.
DR GO; GO:0009789; P:positive regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0045995; P:regulation of embryonic development; IMP:UniProtKB.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0010262; P:somatic embryogenesis; IMP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:UniProtKB.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR044800; LEC2-like.
DR PANTHER; PTHR31140; PTHR31140; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..313
FT /note="B3 domain-containing transcription factor FUS3"
FT /id="PRO_0000375089"
FT DNA_BIND 92..194
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT MUTAGEN 55
FT /note="S->A: Increased seed abortion and delayed embryo
FT development; when associated with A-56 and A-57."
FT /evidence="ECO:0000269|PubMed:28922765"
FT MUTAGEN 55
FT /note="S->D: Delay in flowering; when associated with D-56
FT and D-57."
FT /evidence="ECO:0000269|PubMed:28922765"
FT MUTAGEN 56
FT /note="S->A: Increased seed abortion and delayed embryo
FT development; when associated with A-55 and A-57."
FT /evidence="ECO:0000269|PubMed:28922765"
FT MUTAGEN 56
FT /note="S->D: Delay in flowering; when associated with D-55
FT and D-57."
FT /evidence="ECO:0000269|PubMed:28922765"
FT MUTAGEN 57
FT /note="S->A: Increased seed abortion and delayed embryo
FT development; when associated with A-55 and A-56."
FT /evidence="ECO:0000269|PubMed:28922765"
FT MUTAGEN 57
FT /note="S->D: Delay in flowering; when associated with D-55
FT and D-56."
FT /evidence="ECO:0000269|PubMed:28922765"
FT CONFLICT 10
FT /note="K -> N (in Ref. 1; AAC35246)"
FT /evidence="ECO:0000305"
FT CONFLICT 75
FT /note="P -> T (in Ref. 1; AAC35246)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="E -> G (in Ref. 1; AAC35246)"
FT /evidence="ECO:0000305"
FT CONFLICT 239..241
FT /note="Missing (in Ref. 4; CAG25870/AAS79559)"
FT /evidence="ECO:0000305"
FT CONFLICT 244..245
FT /note="Missing (in Ref. 1; AAC35246)"
FT /evidence="ECO:0000305"
FT STRAND 90..95
FT /evidence="ECO:0007829|PDB:6J9B"
FT HELIX 98..101
FT /evidence="ECO:0007829|PDB:6J9B"
FT HELIX 111..117
FT /evidence="ECO:0007829|PDB:6J9B"
FT STRAND 127..137
FT /evidence="ECO:0007829|PDB:6J9B"
FT STRAND 139..149
FT /evidence="ECO:0007829|PDB:6J9B"
FT STRAND 152..159
FT /evidence="ECO:0007829|PDB:6J9B"
FT HELIX 161..167
FT /evidence="ECO:0007829|PDB:6J9B"
FT STRAND 174..179
FT /evidence="ECO:0007829|PDB:6J9B"
FT TURN 181..183
FT /evidence="ECO:0007829|PDB:6J9B"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:6J9B"
SQ SEQUENCE 313 AA; 35616 MW; 7FBDD73B99ED7075 CRC64;
MMVDENVETK ASTLVASVDH GFGSGSGHDH HGLSASVPLL GVNWKKRRMP RQRRSSSSFN
LLSFPPPMPP ISHVPTPLPA RKIDPRKLRF LFQKELKNSD VSSLRRMILP KKAAEAHLPA
LECKEGIPIR MEDLDGFHVW TFKYRYWPNN NSRMYVLENT GDFVNAHGLQ LGDFIMVYQD
LYSNNYVIQA RKASEEEEVD VINLEEDDVY TNLTRIENTV VNDLLLQDFN HHNNNNNNNS
NSNSNKCSYY YPVIDDVTTN TESFVYDTTA LTSNDTPLDF LGGHTTTTNN YYSKFGTFDG
LGSVENISLD DFY
