ID   FUS5_GIBF5              Reviewed;         233 AA.
AC   S0EES1;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2013, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Esterase FUS5 {ECO:0000250|UniProtKB:Q0C8M2};
DE            EC=3.1.2.- {ECO:0000305};
DE   AltName: Full=Fusarin biosynthesis protein 5 {ECO:0000303|PubMed:23932525};
GN   Name=FUS5 {ECO:0000303|PubMed:23932525}; ORFNames=FFUJ_10054;
OS   Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS   and foot rot disease fungus) (Fusarium fujikuroi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=1279085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX   PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA   Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA   Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA   Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA   Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA   Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA   Tudzynski B.;
RT   "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT   Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT   novel metabolites.";
RL   PLoS Pathog. 9:E1003475-E1003475(2013).
RN   [2]
RP   INDUCTION.
RX   PubMed=20572938; DOI=10.1111/j.1365-2958.2010.07263.x;
RA   Wiemann P., Brown D.W., Kleigrewe K., Bok J.W., Keller N.P., Humpf H.U.,
RA   Tudzynski B.;
RT   "FfVel1 and FfLae1, components of a velvet-like complex in Fusarium
RT   fujikuroi, affect differentiation, secondary metabolism and virulence.";
RL   Mol. Microbiol. 77:972-994(2010).
RN   [3]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23932525; DOI=10.1016/j.chembiol.2013.07.004;
RA   Niehaus E.M., Kleigrewe K., Wiemann P., Studt L., Sieber C.M.,
RA   Connolly L.R., Freitag M., Gueldener U., Tudzynski B., Humpf H.U.;
RT   "Genetic manipulation of the Fusarium fujikuroi fusarin gene cluster yields
RT   insight into the complex regulation and fusarin biosynthetic pathway.";
RL   Chem. Biol. 20:1055-1066(2013).
CC   -!- FUNCTION: Esterase; part of the gene cluster that mediates the
CC       biosynthesis of the mycotoxin fusarin C (PubMed:23932525). Within the
CC       cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin
CC       production (PubMed:23932525). The other FUS cluster members are not
CC       essential for fusarin C biosynthesis (PubMed:23932525).
CC       {ECO:0000269|PubMed:23932525}.
CC   -!- INDUCTION: Expressed under high amounts of nitrogen via regulation by
CC       GLN1 (PubMed:23932525). Moreover, components of the fungal-specific
CC       velvet complex VEL1, VEL2 and LAE1 act also as positive regulators of
CC       expression (PubMed:20572938, PubMed:23932525). Finally, expression is
CC       induced under acidic conditions in a PACC-independent manner
CC       (PubMed:23932525). {ECO:0000269|PubMed:20572938,
CC       ECO:0000269|PubMed:23932525}.
CC   -!- DISRUPTION PHENOTYPE: Does not alter fusarin C production
CC       (PubMed:23932525). {ECO:0000269|PubMed:23932525}.
CC   -!- SIMILARITY: Belongs to the LovG family. {ECO:0000305}.
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DR   EMBL; HF679031; CCT73264.1; -; Genomic_DNA.
DR   AlphaFoldDB; S0EES1; -.
DR   SMR; S0EES1; -.
DR   STRING; 1279085.S0EES1; -.
DR   EnsemblFungi; CCT73264; CCT73264; FFUJ_10054.
DR   VEuPathDB; FungiDB:FFUJ_10054; -.
DR   HOGENOM; CLU_051938_0_2_1; -.
DR   Proteomes; UP000016800; Chromosome 9.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005645; FSH_dom.
DR   Pfam; PF03959; FSH1; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..233
FT                   /note="Esterase FUS5"
FT                   /id="PRO_0000437369"
FT   ACT_SITE        105
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        159
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        187
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
SQ   SEQUENCE   233 AA;  26150 MW;  9EA33150D35A02D8 CRC64;
     MVHRPRLLCL HGGGASSQIM RVQFSKLESA LRKTFQLVFL EGPLDSAPGP GVLPFFEDFG
     PYSCWVSDDK SLLPEEKRKE ENNAIAYIKT FMLQYGPFAG ILGFSQGARA TASILLEQQR
     EAFTHDALFG VFFCGTFPPF IPDAPDISLP TVHILGLTDP YLRESEELLE HCTQQSVRRV
     IKFNGGHHMP TSSDVTQKIA DVITMTYRTS QRKKVSNIWR KKVPDCRSLA LES