FUSIL_DROME
ID FUSIL_DROME Reviewed; 967 AA.
AC Q9BJZ5; A1ZA30; A1ZA31; Q1EBX1; Q7JR39; Q960H4;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=RNA-binding protein fusilli;
GN Name=fus; ORFNames=CG8205;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D), DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=11133153; DOI=10.1006/dbio.2000.9954;
RA Wakabayashi-Ito N., Belvin M.P., Bluestein D.A., Anderson K.V.;
RT "fusilli, an essential gene with a maternal role in Drosophila embryonic
RT dorsal-ventral patterning.";
RL Dev. Biol. 229:44-54(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-694 (ISOFORM D).
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 527-967 (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP FUNCTION.
RX PubMed=19285943; DOI=10.1016/j.molcel.2009.01.025;
RA Warzecha C.C., Sato T.K., Nabet B., Hogenesch J.B., Carstens R.P.;
RT "ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2
RT splicing.";
RL Mol. Cell 33:591-601(2009).
CC -!- FUNCTION: mRNA splicing factor that regulates alternative splicing od
CC specific genes. Has a maternal role in embryonic dorsal-ventral
CC patterning; such role is probably indirect and due to its function as
CC mRNA splicing factor regulator. {ECO:0000269|PubMed:19285943}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=D; Synonyms=F;
CC IsoId=Q9BJZ5-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q9BJZ5-2; Sequence=VSP_036959, VSP_036960;
CC Name=C;
CC IsoId=Q9BJZ5-3; Sequence=VSP_036960;
CC Name=B;
CC IsoId=Q9BJZ5-4; Sequence=VSP_036959;
CC -!- DEVELOPMENTAL STAGE: Not present in the oocyte or early embryo.
CC Expressed in a number of embryonic tissues at later stages. First
CC detected at stage 9 of embryogenesis, in the epithelium of the
CC stomodeum, which develops into the foregut and in the epithelium of the
CC proctodeal opening. Segmentally repeated expression was observed in the
CC mesodermal layer in stage 11. At stage 13, segmentally repeated
CC ectodermal expression is observed. Also expressed in the epithelium of
CC the foregut and posterior spiracles and the fat body. Detected in both
CC nurse cells and follicle cells during oogenesis. Enriched in follicle
CC cells adjacent to the anterior end of the oocyte.
CC {ECO:0000269|PubMed:11133153}.
CC -!- DISRUPTION PHENOTYPE: Death during embryogenesis; without displaying
CC dorsal-ventral patterning defects. Heterozygous embryos act as a
CC dominant maternal enhancer of dorsalizing mutation cact(E10) and
CC display a partially dorsalized phenotype.
CC {ECO:0000269|PubMed:11133153}.
CC -!- SIMILARITY: Belongs to the ESRP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK93488.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF321979; AAK15280.1; -; mRNA.
DR EMBL; AE013599; AAF58117.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70980.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70981.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70983.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70984.1; -; Genomic_DNA.
DR EMBL; BT025963; ABG02207.1; -; mRNA.
DR EMBL; BT003299; AAO25059.1; -; mRNA.
DR EMBL; BT023793; AAZ41802.1; -; mRNA.
DR EMBL; AY052064; AAK93488.1; ALT_INIT; mRNA.
DR RefSeq; NP_524691.1; NM_079952.4. [Q9BJZ5-1]
DR RefSeq; NP_725479.1; NM_166105.3. [Q9BJZ5-3]
DR RefSeq; NP_725480.1; NM_166106.3. [Q9BJZ5-2]
DR RefSeq; NP_725481.1; NM_166107.2. [Q9BJZ5-1]
DR RefSeq; NP_725482.1; NM_166108.3. [Q9BJZ5-1]
DR RefSeq; NP_725483.1; NM_166109.2. [Q9BJZ5-4]
DR AlphaFoldDB; Q9BJZ5; -.
DR SMR; Q9BJZ5; -.
DR BioGRID; 68827; 5.
DR IntAct; Q9BJZ5; 3.
DR STRING; 7227.FBpp0086476; -.
DR PaxDb; Q9BJZ5; -.
DR ABCD; Q9BJZ5; 1 sequenced antibody.
DR DNASU; 44095; -.
DR EnsemblMetazoa; FBtr0087343; FBpp0086476; FBgn0023441. [Q9BJZ5-1]
DR EnsemblMetazoa; FBtr0087344; FBpp0086477; FBgn0023441. [Q9BJZ5-1]
DR EnsemblMetazoa; FBtr0087345; FBpp0086478; FBgn0023441. [Q9BJZ5-3]
DR EnsemblMetazoa; FBtr0087346; FBpp0086479; FBgn0023441. [Q9BJZ5-1]
DR EnsemblMetazoa; FBtr0087347; FBpp0086480; FBgn0023441. [Q9BJZ5-4]
DR EnsemblMetazoa; FBtr0087348; FBpp0086481; FBgn0023441. [Q9BJZ5-2]
DR GeneID; 44095; -.
DR KEGG; dme:Dmel_CG8205; -.
DR UCSC; CG8205-RA; d. melanogaster.
DR UCSC; CG8205-RB; d. melanogaster.
DR UCSC; CG8205-RC; d. melanogaster.
DR UCSC; CG8205-RD; d. melanogaster. [Q9BJZ5-1]
DR CTD; 2521; -.
DR FlyBase; FBgn0023441; fus.
DR VEuPathDB; VectorBase:FBgn0023441; -.
DR eggNOG; KOG1365; Eukaryota.
DR eggNOG; KOG4211; Eukaryota.
DR GeneTree; ENSGT00940000169473; -.
DR HOGENOM; CLU_008009_0_1_1; -.
DR InParanoid; Q9BJZ5; -.
DR PhylomeDB; Q9BJZ5; -.
DR Reactome; R-DME-6803529; FGFR2 alternative splicing.
DR BioGRID-ORCS; 44095; 0 hits in 3 CRISPR screens.
DR ChiTaRS; fus; fly.
DR GenomeRNAi; 44095; -.
DR PRO; PR:Q9BJZ5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0023441; Expressed in crop (Drosophila) and 44 other tissues.
DR ExpressionAtlas; Q9BJZ5; baseline and differential.
DR Genevisible; Q9BJZ5; DM.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0043484; P:regulation of RNA splicing; IDA:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd12741; RRM2_Fusilli; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR034980; Fusilli_RRM2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000504; RRM_dom.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 3.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..967
FT /note="RNA-binding protein fusilli"
FT /id="PRO_0000370633"
FT DOMAIN 279..356
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 380..464
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 65..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 673..716
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 673..694
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..334
FT /note="Missing (in isoform A and isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569, ECO:0000303|Ref.4"
FT /id="VSP_036959"
FT VAR_SEQ 669..967
FT /note="MFSSAGQSPTTVAAGTAQSPLGGTHTHTHPHSHAHAHATGHAHAAAAAAHHG
FT LSASSAMLPGLSAAASASGLASFMSAGQQSAAAAAHSAASLQNAAVALAPQGYALNPFS
FT LPPPGSAAAAAASTPALLAQQQAQFIAQQSLLVRQQAAAAALAAEQQQQQQQQQQLYAS
FT AMLQQHPLYLQQQQQQQQLYASAMLQQGQPQFVLMQRPSAAYLPPFPLSYMSAAGAASG
FT VGVAPGAAVAGAAAAASPSASNSSLSQSMKRSYENAFQQEAAGAAAAASAAKRALTRQP
FT SSVYSYYNSGI -> ATVLGAHYGGPFPAYGAAPPPPPPHTPLLATPRSHHHPHHHPAS
FT AFYPAPLVYWPYPSPPVSPTTYYSQPGAHSPSQAMYPLDFFPPSPLSPPNVSIPNTSTG
FT LILTPTKAAVSFVPPKQFSPSSTGSGSPSSSSVKLAGQAGPAHMPSIAVNGKTEGVTST
FT GAKDTSTPQETSASQTLLSIDTNHATPTTAAAPPLKTGSQGSAGAAATPLVTNSCVELY
FT IS (in isoform A and isoform C)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_036960"
SQ SEQUENCE 967 AA; 102743 MW; BAF72EB237768B86 CRC64;
MQVPEHVVSL YIATCGQNGS GLGSDEKEII LLVFVLLEVS TGQIVGTKQI LVRPDGYFIK
DRTISSSSDN SSVTNNTASS PPLAIGDANN GSGSTSGNGN SLENGSELIL PIAEAQAAGK
PLSEAIEEFD AYLRSLSLHD TEIKLVTDGQ LPLRQCLHRE ASAKDVELPA YYNRFSDLRK
EFLRYKSGDL ARALVPVKDV KKMLQAPTLP MPQSIAEMLG ELNISSVEDN DFYIRESRDM
VTVIQTLLQA GHKFAANELV NLVLEPGICS IDDEVDGNCI VRARGLPWQS SDQDIAKFFR
GLNVAKGGVA LCLSPLGRRN GEALIRFVCQ EHRDMALKRH KHHIGTRYIE VYRASGEDFL
AIAGGASNEA QAFLSKGAQV IIRMRGLPYD ATAKQVLDFF TTGDTPPCHV LDGNEGVLFV
KKPDGRATGD AFVLFANETD APKALGRHRE SIGQRYIELF RSTTAEVQQV LNRSMDPKNY
ESGGGHSQPP LIAQLPTMQL PLLPQVGAHS LSHSLGASPA NLCPPVPPPA LPLPTQHLIT
SGTTKNCIRL RGLPYEAMVE HILHFLDDFA KHIIYQGVHM VINAQGQPSG EAFIQMDLEE
SARLCAQRRH NHYMMFGKKY RYIEVFQCSG DDMNMVLNGG LASPVAQPPP PHLGHAHKQQ
SLLAATTGMF SSAGQSPTTV AAGTAQSPLG GTHTHTHPHS HAHAHATGHA HAAAAAAHHG
LSASSAMLPG LSAAASASGL ASFMSAGQQS AAAAAHSAAS LQNAAVALAP QGYALNPFSL
PPPGSAAAAA ASTPALLAQQ QAQFIAQQSL LVRQQAAAAA LAAEQQQQQQ QQQQLYASAM
LQQHPLYLQQ QQQQQQLYAS AMLQQGQPQF VLMQRPSAAY LPPFPLSYMS AAGAASGVGV
APGAAVAGAA AAASPSASNS SLSQSMKRSY ENAFQQEAAG AAAAASAAKR ALTRQPSSVY
SYYNSGI
