FXA4B_XENLA
ID FXA4B_XENLA Reviewed; 400 AA.
AC P33206; Q640I4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Forkhead box protein A4-B;
DE Short=FoxA4-B;
DE Short=FoxA4b;
DE AltName: Full=Fork head domain-related protein 1';
DE Short=FKH-1;
DE Short=Forkhead protein 1;
DE Short=xFD-1';
DE Short=xFKH1;
GN Name=foxa4-b; Synonyms=fkh1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP INDUCTION.
RC TISSUE=Embryo;
RX PubMed=1559610; DOI=10.1101/gad.6.4.599;
RA Dirksen M.L., Jamrich M.;
RT "A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis
RT contains a fork head DNA-binding domain.";
RL Genes Dev. 6:599-608(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC TISSUE=Gastrula;
RX PubMed=1358174; DOI=10.1016/0925-4773(92)90007-7;
RA Knoechel S., Lef J., Clement J.H., Klocke B., Hille S., Koester M.,
RA Knoechel W.;
RT "Activin A induced expression of a fork head related gene in posterior
RT chordamesoderm (notochord) of Xenopus laevis embryos.";
RL Mech. Dev. 38:157-165(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INDUCTION.
RX PubMed=8978699; DOI=10.1002/j.1460-2075.1996.tb01063.x;
RA Kaufmann E., Paul H., Friedle H., Metz A., Scheucher M., Clement J.H.,
RA Knoechel W.;
RT "Antagonistic actions of activin A and BMP-2/4 control dorsal lip-specific
RT activation of the early response gene XFD-1' in Xenopus laevis embryos.";
RL EMBO J. 15:6739-6749(1996).
RN [5]
RP INDUCTION.
RX PubMed=9545242; DOI=10.1093/emboj/17.8.2298;
RA Friedle H., Rastegar S., Paul H., Kaufmann E., Knoechel W.;
RT "Xvent-1 mediates BMP-4-induced suppression of the dorsal-lip-specific
RT early response gene XFD-1' in Xenopus embryos.";
RL EMBO J. 17:2298-2307(1998).
RN [6]
RP PUTATIVE FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11291856;
RA El-Hodiri H., Bhatia-Dey N., Kenyon K., Ault K., Dirksen M.-L., Jamrich M.;
RT "Fox (forkhead) genes are involved in the dorso-ventral patterning of the
RT Xenopus mesoderm.";
RL Int. J. Dev. Biol. 45:265-271(2001).
RN [7]
RP REVIEW.
RX PubMed=15656969; DOI=10.1016/j.gene.2004.09.037;
RA Pohl B.S., Knoechel W.;
RT "Of fox and frogs: fox (fork head/winged helix) transcription factors in
RT Xenopus development.";
RL Gene 344:21-32(2005).
CC -!- FUNCTION: Transcriptional repressor involved in embryonic nervous
CC system development. Plays a role in the induction and patterning of the
CC anterior-posterior neural axis. Involved in the establishment of floor
CC plate differentiation from neural plate cells during gastrulation.
CC Binds the anf1 promoter sequence to restrict expression of anf1 to the
CC anterior of the neural plate, thereby patterning the forebrain. Can
CC bind to the HNF-3-alpha DNA target sequence. Cooperates with t/bra in a
CC dose-dependent manner to specify dorsal mesoderm formation, including
CC notochord. May be involved in the dorso-ventral patterning of the
CC mesoderm. Binds to DNA via the target sequence 5'-[GA]TAAA[TC]A-3',
CC with 5'-GTAAATA-3' being the preferred binding site (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Primarily expressed in the dorsal blastopore lip
CC (Spemann organizer) of early gastrulae. At later stages, expressed in
CC the dorsal mesoderm and the neural floor plate. In the dorsal mesoderm,
CC expressed in the notochord but not in the presomitic mesoderm. Also
CC expressed in the mid-brain area. {ECO:0000269|PubMed:11291856,
CC ECO:0000269|PubMed:1559610}.
CC -!- DEVELOPMENTAL STAGE: First expressed shortly after the mid blastula
CC transition. Most abundant during gastrulation (stages 10 and 12).
CC {ECO:0000269|PubMed:1559610}.
CC -!- INDUCTION: By activin. Down-regulated indirectly by bmp-4 signaling,
CC mediated via vent1. {ECO:0000269|PubMed:1559610,
CC ECO:0000269|PubMed:8978699, ECO:0000269|PubMed:9545242}.
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DR EMBL; S93559; AAB22027.1; -; mRNA.
DR EMBL; U65750; AAD03481.1; -; Genomic_DNA.
DR EMBL; BC082641; AAH82641.1; -; mRNA.
DR PIR; A42237; A42237.
DR RefSeq; NP_001081076.1; NM_001087607.1.
DR AlphaFoldDB; P33206; -.
DR SMR; P33206; -.
DR DNASU; 394368; -.
DR GeneID; 394368; -.
DR KEGG; xla:394368; -.
DR CTD; 394368; -.
DR Xenbase; XB-GENE-6254437; foxa4.S.
DR OrthoDB; 489982at33208; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 394368; Expressed in gastrula and 2 other tissues.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0019904; F:protein domain specific binding; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR013638; Fork-head_N.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018533; Forkhead_box_C.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR Pfam; PF08430; Forkhead_N; 1.
DR Pfam; PF09354; HNF_C; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..400
FT /note="Forkhead box protein A4-B"
FT /id="PRO_0000091898"
FT DNA_BIND 119..213
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 218..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..235
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 245..290
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 400 AA; 44485 MW; EE8EEAF3AC9EE746 CRC64;
MLNRVKLELK DPMDWNTMYQ ENEIYSGIHN MTNGLPSNSF LPTDVPTVTS SMTYMSNGLP
GPVASIQGNL GSLGSMTQGM VGSLAPPPST SAYPLGYCQG ESEFQRDPRT YRRNYSHAKP
PYSYISLITM AIQQAPNKMM TLNEIYQWIV DLFPYYRQNQ QRWQNSIRHS LSFNDCFIKV
PRSPEKPGKG SYWTLHPESG NMFENGCYLR RQKRFKCERS KSGEGERKGN KPGDETGGSL
KETPVSFDDC SSSRSPQAAV NDGGRDSTGS SIHQATGGSP VGFSPTSEQA GTASQLMYPL
GLSNDGYLGL VGEDVHLKHD PFSGRHPFSI TQLMSSEQDQ TYPNKMEMCP TTDHLVHYSN
YSSDYHNLVS KNGLDMQTSS SSTDNGYYAN MYSRPILSSL
