ID   FXD3B_XENLA             Reviewed;         371 AA.
AC   Q9DEN3;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Forkhead box protein D3-B;
DE            Short=FoxD3-B;
DE            Short=FoxD3b;
DE   AltName: Full=Fork head domain-related protein 6';
DE            Short=xFD-6';
GN   Name=foxd3-b; Synonyms=foxd3b {ECO:0000312|EMBL:CAC12895.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAC12895.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND INDUCTION.
RC   TISSUE=Gastrula {ECO:0000269|PubMed:11335115};
RX   PubMed=11335115; DOI=10.1016/s0925-4773(01)00334-3;
RA   Pohl B.S., Knoechel W.;
RT   "Overexpression of the transcriptional repressor FoxD3 prevents neural
RT   crest formation in Xenopus embryos.";
RL   Mech. Dev. 103:93-106(2001).
RN   [2] {ECO:0000312|EMBL:AAH72772.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo {ECO:0000312|EMBL:AAH72772.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305}
RP   REVIEW.
RX   PubMed=15656969; DOI=10.1016/j.gene.2004.09.037;
RA   Pohl B.S., Knoechel W.;
RT   "Of fox and frogs: fox (fork head/winged helix) transcription factors in
RT   Xenopus development.";
RL   Gene 344:21-32(2005).
CC   -!- FUNCTION: Transcriptional repressor that is an essential upstream
CC       regulator of neural crest determination and mesoderm induction.
CC       Functions by recruiting the transcriptional corepressor tle4. Also acts
CC       in a negative auto-regulator loop by inhibiting the transcription of
CC       its own gene. {ECO:0000269|PubMed:11335115}.
CC   -!- SUBUNIT: Interacts with tle4. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Initially located within the dorsal blastopore lip
CC       (Spemann organizer) at the mid-gastrula stage. As expression fades in
CC       the neuroectoderm, expression begins in the head mesoderm in two
CC       regions at the lateral borders of the presumptive neural plate. These
CC       regions correspond to neural crest cells originating from the
CC       mesencephalon (anterior) and the rhombencephalon (posterior;
CC       rhombomeres 2, 4 and 6). Subsequently expressed within trunk
CC       premigratory neural crest cells. Expression disappears when neural
CC       crest cells begin to differentiate and migrate, except for those which
CC       populate the hyoid arch. {ECO:0000269|PubMed:11335115}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC       Maternal expression decreases until the late blastula/early gastrula
CC       stage when zygotic expression begins. Expressed at an almost constant
CC       level through the subsequent developmental stages.
CC       {ECO:0000269|PubMed:11335115}.
CC   -!- INDUCTION: By dorsal wnt signaling. Inhibited by bmp signaling.
CC       {ECO:0000269|PubMed:11335115}.
CC   -!- DOMAIN: The C-terminus is required for transcriptional repression and
CC       mesoderm induction. {ECO:0000250}.
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DR   EMBL; AJ298866; CAC12895.1; -; mRNA.
DR   EMBL; BC072772; AAH72772.1; -; mRNA.
DR   RefSeq; NP_001079078.1; NM_001085609.1.
DR   AlphaFoldDB; Q9DEN3; -.
DR   SMR; Q9DEN3; -.
DR   DNASU; 373610; -.
DR   GeneID; 373610; -.
DR   KEGG; xla:373610; -.
DR   CTD; 373610; -.
DR   Xenbase; XB-GENE-6252892; foxd3.S.
DR   OrthoDB; 1270467at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 373610; Expressed in internal ear and 12 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0050768; P:negative regulation of neurogenesis; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   GO; GO:0014034; P:neural crest cell fate commitment; ISS:UniProtKB.
DR   GO; GO:0050769; P:positive regulation of neurogenesis; ISS:UniProtKB.
DR   CDD; cd00059; FH; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001766; Fork_head_dom.
DR   InterPro; IPR030456; TF_fork_head_CS_2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00250; Forkhead; 1.
DR   PRINTS; PR00053; FORKHEAD.
DR   SMART; SM00339; FH; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00658; FORK_HEAD_2; 1.
DR   PROSITE; PS50039; FORK_HEAD_3; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..371
FT                   /note="Forkhead box protein D3-B"
FT                   /id="PRO_0000250609"
FT   DNA_BIND        94..188
FT                   /note="Fork-head"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT   REGION          1..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          297..303
FT                   /note="tle4-binding"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        30..56
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..90
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   371 AA;  40148 MW;  2638CA527AD531C4 CRC64;
     MTLSSSGSAS DMSGQTVLSA DDADIDVVGE GDEALDKDSE CESPVGHHDE VDALGGKEIP
     RSPSGSSTDA EGKGESQQQQ QEGIQNKPKN SVVKPPYSYI ALITMSILQS PQKKLTLSGI
     CEFISSRFPY YREKFPAWQN SIRHNLSLND CFIKIPREPG NPGKGNYWTL DPQSEDMFDN
     GSFLRRRKRF KRQQQDTLRE QTALMMQSFG AYSLASPYGR HYGLHPAAYT HPAALQYPYI
     PPVGPMLPPA VPLLPSSELT RKAFSSQLSP SLQLQLSSLS STAASIIKSE PSSRPSFSIE
     NIIGVSAASS VAPQTFLRPP VTVQSALMSH QPLVLSRSTA AIGPILSVPT NLISGQFLPT
     AATAVAKWPA Q