FXL14_HUMAN
ID FXL14_HUMAN Reviewed; 418 AA.
AC Q8N1E6;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=F-box/LRR-repeat protein 14;
DE AltName: Full=F-box and leucine-rich repeat protein 14;
GN Name=FBXL14; Synonyms=FBL14;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP FUNCTION, INTERACTION WITH SNAI1, SUBCELLULAR LOCATION, AND INDUCTION BY
RP HYPOXIA.
RX PubMed=19955572; DOI=10.1074/jbc.m109.065995;
RA Vinas-Castells R., Beltran M., Valls G., Gomez I., Garcia J.M.,
RA Montserrat-Sentis B., Baulida J., Bonilla F., de Herreros A.G., Diaz V.M.;
RT "The hypoxia-controlled FBXL14 ubiquitin ligase targets SNAIL1 for
RT proteasome degradation.";
RL J. Biol. Chem. 285:3794-3805(2010).
CC -!- FUNCTION: Substrate-recognition component of some SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin-protein ligase complexes. The SCF(FBXL14)
CC complex acts by mediating ubiquitination and subsequent degradation of
CC SNAI1. {ECO:0000269|PubMed:19955572}.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase
CC complex. Interacts with SKP1 and CUL1 (By similarity). Interacts with
CC SNAI1; the interaction requires the phosphorylation of the two serine
CC residues in the substrate destruction motif D-S-G-X(2,3,4)-S.
CC {ECO:0000250, ECO:0000269|PubMed:19955572}.
CC -!- INTERACTION:
CC Q8N1E6; P63208: SKP1; NbExp=5; IntAct=EBI-6425532, EBI-307486;
CC Q8N1E6; O95863: SNAI1; NbExp=2; IntAct=EBI-6425532, EBI-1045459;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19955572}.
CC -!- INDUCTION: Down-regulated by hypoxia. {ECO:0000269|PubMed:19955572}.
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DR EMBL; BC028132; AAH28132.1; -; mRNA.
DR CCDS; CCDS8509.1; -.
DR RefSeq; NP_689654.1; NM_152441.2.
DR AlphaFoldDB; Q8N1E6; -.
DR SMR; Q8N1E6; -.
DR BioGRID; 126870; 36.
DR IntAct; Q8N1E6; 31.
DR MINT; Q8N1E6; -.
DR STRING; 9606.ENSP00000344855; -.
DR GlyGen; Q8N1E6; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8N1E6; -.
DR PhosphoSitePlus; Q8N1E6; -.
DR BioMuta; FBXL14; -.
DR DMDM; 48428083; -.
DR EPD; Q8N1E6; -.
DR MassIVE; Q8N1E6; -.
DR MaxQB; Q8N1E6; -.
DR PaxDb; Q8N1E6; -.
DR PeptideAtlas; Q8N1E6; -.
DR PRIDE; Q8N1E6; -.
DR ProteomicsDB; 71590; -.
DR Antibodypedia; 41744; 111 antibodies from 22 providers.
DR DNASU; 144699; -.
DR Ensembl; ENST00000339235.4; ENSP00000344855.3; ENSG00000171823.7.
DR GeneID; 144699; -.
DR KEGG; hsa:144699; -.
DR MANE-Select; ENST00000339235.4; ENSP00000344855.3; NM_152441.3; NP_689654.1.
DR UCSC; uc001qjh.3; human.
DR CTD; 144699; -.
DR DisGeNET; 144699; -.
DR GeneCards; FBXL14; -.
DR HGNC; HGNC:28624; FBXL14.
DR HPA; ENSG00000171823; Low tissue specificity.
DR MIM; 609081; gene.
DR neXtProt; NX_Q8N1E6; -.
DR OpenTargets; ENSG00000171823; -.
DR PharmGKB; PA134979350; -.
DR VEuPathDB; HostDB:ENSG00000171823; -.
DR eggNOG; KOG1947; Eukaryota.
DR GeneTree; ENSGT00940000159857; -.
DR HOGENOM; CLU_016072_7_0_1; -.
DR InParanoid; Q8N1E6; -.
DR OMA; DQALVHI; -.
DR OrthoDB; 1282076at2759; -.
DR PhylomeDB; Q8N1E6; -.
DR TreeFam; TF313434; -.
DR PathwayCommons; Q8N1E6; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q8N1E6; -.
DR BioGRID-ORCS; 144699; 14 hits in 1116 CRISPR screens.
DR ChiTaRS; FBXL14; human.
DR GenomeRNAi; 144699; -.
DR Pharos; Q8N1E6; Tbio.
DR PRO; PR:Q8N1E6; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q8N1E6; protein.
DR Bgee; ENSG00000171823; Expressed in left testis and 112 other tissues.
DR Genevisible; Q8N1E6; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF13516; LRR_6; 5.
DR SMART; SM00367; LRR_CC; 11.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Leucine-rich repeat; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..418
FT /note="F-box/LRR-repeat protein 14"
FT /id="PRO_0000119861"
FT DOMAIN 2..48
FT /note="F-box"
FT REPEAT 144..163
FT /note="LRR 1"
FT REPEAT 170..191
FT /note="LRR 2"
FT REPEAT 203..225
FT /note="LRR 3"
FT REPEAT 229..250
FT /note="LRR 4"
FT REPEAT 254..275
FT /note="LRR 5"
FT REGION 2..48
FT /note="Required for down-regulation of SNAI1"
FT VARIANT 220
FT /note="L -> V (in dbSNP:rs35571553)"
FT /id="VAR_049033"
SQ SEQUENCE 418 AA; 45886 MW; 5779961C8177779F CRC64;
METHISCLFP ELLAMIFGYL DVRDKGRAAQ VCTAWRDAAY HKSVWRGVEA KLHLRRANPS
LFPSLQARGI RRVQILSLRR SLSYVIQGMA NIESLNLSGC YNLTDNGLGH AFVQEIGSLR
ALNLSLCKQI TDSSLGRIAQ YLKGLEVLEL GGCSNITNTG LLLIAWGLQR LKSLNLRSCR
HLSDVGIGHL AGMTRSAAEG CLGLEQLTLQ DCQKLTDLSL KHISRGLTGL RLLNLSFCGG
ISDAGLLHLS HMGSLRSLNL RSCDNISDTG IMHLAMGSLR LSGLDVSFCD KVGDQSLAYI
AQGLDGLKSL SLCSCHISDD GINRMVRQMH GLRTLNIGQC VRITDKGLEL IAEHLSQLTG
IDLYGCTRIT KRGLERITQL PCLKVLNLGL WQMTDSEKEA RGDFSPLFTV RTRGSSRR
