FXL14_MOUSE
ID FXL14_MOUSE Reviewed; 400 AA.
AC Q8BID8; Q3U2Q9; Q8R5H7; Q8VDT7; Q922N5;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=F-box/LRR-repeat protein 14;
DE AltName: Full=F-box and leucine-rich repeat protein 14;
GN Name=Fbxl14; Synonyms=Fbl14, Ppa;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Heart, and Mammary tumor;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 207-390.
RX PubMed=11976951; DOI=10.1007/s00427-002-0222-7;
RA Das T., Purkayastha-Mukherjee C., D'Angelo J., Weir M.;
RT "A conserved F-box gene with unusual transcript localization.";
RL Dev. Genes Evol. 212:134-140(2002).
CC -!- FUNCTION: Substrate-recognition component of some (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin-protein ligase complexes. The SCF(FBXL14)
CC complex acts by mediating ubiquitination and subsequent degradation of
CC SNAI1 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase
CC complex. Interacts with SKP1 and CUL1. Interacts with SNAI1; the
CC interaction requires the phosphorylation of the two serine residues in
CC the substrate destruction motif D-S-G-X(2,3,4)-S (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; AK084506; BAC39201.1; -; mRNA.
DR EMBL; AK155154; BAE33081.1; -; mRNA.
DR EMBL; BC006913; AAH06913.1; -; mRNA.
DR EMBL; BC021329; AAH21329.1; -; mRNA.
DR EMBL; AF467463; AAL75967.1; -; mRNA.
DR CCDS; CCDS20475.1; -.
DR RefSeq; NP_598701.2; NM_133940.3.
DR AlphaFoldDB; Q8BID8; -.
DR SMR; Q8BID8; -.
DR STRING; 10090.ENSMUSP00000032094; -.
DR iPTMnet; Q8BID8; -.
DR PhosphoSitePlus; Q8BID8; -.
DR EPD; Q8BID8; -.
DR PaxDb; Q8BID8; -.
DR PRIDE; Q8BID8; -.
DR ProteomicsDB; 267535; -.
DR Antibodypedia; 41744; 111 antibodies from 22 providers.
DR DNASU; 101358; -.
DR Ensembl; ENSMUST00000032094; ENSMUSP00000032094; ENSMUSG00000030019.
DR GeneID; 101358; -.
DR KEGG; mmu:101358; -.
DR UCSC; uc009dmj.2; mouse.
DR CTD; 144699; -.
DR MGI; MGI:2141676; Fbxl14.
DR VEuPathDB; HostDB:ENSMUSG00000030019; -.
DR eggNOG; KOG1947; Eukaryota.
DR GeneTree; ENSGT00940000159857; -.
DR HOGENOM; CLU_016072_7_0_1; -.
DR InParanoid; Q8BID8; -.
DR OMA; DQALVHI; -.
DR OrthoDB; 1282076at2759; -.
DR PhylomeDB; Q8BID8; -.
DR TreeFam; TF313434; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR BioGRID-ORCS; 101358; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Fbxl14; mouse.
DR PRO; PR:Q8BID8; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8BID8; protein.
DR Bgee; ENSMUSG00000030019; Expressed in granulocyte and 247 other tissues.
DR Genevisible; Q8BID8; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF13516; LRR_6; 5.
DR SMART; SM00367; LRR_CC; 11.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Leucine-rich repeat; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..400
FT /note="F-box/LRR-repeat protein 14"
FT /id="PRO_0000119862"
FT DOMAIN 2..48
FT /note="F-box"
FT REPEAT 144..163
FT /note="LRR 1"
FT REPEAT 170..191
FT /note="LRR 2"
FT REPEAT 203..225
FT /note="LRR 3"
FT REPEAT 229..250
FT /note="LRR 4"
FT REPEAT 254..275
FT /note="LRR 5"
FT REGION 2..48
FT /note="Required for down-regulation of SNAI1"
FT /evidence="ECO:0000250"
FT CONFLICT 22
FT /note="V -> F (in Ref. 2; AAH21329)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 400 AA; 43864 MW; E0B297E4B4F83C22 CRC64;
METHISCLFP ELLAMIFGYL DVRDKGRAAQ VCTAWRDAAY HKSVWRGVEA KLHLRRANPS
LFPSLQARGI RRVQILSLRR SLSYVIQGMA NIESLNLSGC YNLTDNGLGH AFVQEIGSLR
ALNLSLCKQI TDSSLGRIAQ YLKGLEVLEL GGCSNITNTG LLLIAWGLQR LKSLNLRSCR
HLSDVGIGHL AGMTRSAAEG CLGLEQLTLQ DCQKLTDLSL KHISRGLTGL RLLNLSFCGG
ISDAGLLHLS HMGSLRSLNL RSCDNISDTG IMHLAMGSLR LSGLDVSFCD KVGDQSLAYI
AQGLDGLKSL SLCSCHISDD GINRMVRQMH GLRTLNIGQC VRITDKGLEL IAEHLSQLTG
IDLYGCTRIT KRGLERITQL PCLKVLNLGL WQMTDSEKVR
