FXSA_ECOLI
ID FXSA_ECOLI Reviewed; 158 AA.
AC P37147; P76797; Q2M6G4;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=UPF0716 protein FxsA;
DE AltName: Full=Suppressor of F exclusion of phage T7;
GN Name=fxsA; Synonyms=yjeG; OrderedLocusNames=b4140, JW4100;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=10497016; DOI=10.1006/jmbi.1999.3087;
RA Wang W.-F., Cheng X., Molineux I.J.;
RT "Isolation and identification of fxsA, an Escherichia coli gene that can
RT suppress F exclusion of bacteriophage T7.";
RL J. Mol. Biol. 292:485-499(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-79.
RC STRAIN=K12;
RX PubMed=3541901; DOI=10.1042/bj2370547;
RA Woods S.A., Miles J.S., Roberts R.E., Guest J.R.;
RT "Structural and functional relationships between fumarase and aspartase.
RT Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of
RT Escherichia coli K12.";
RL Biochem. J. 237:547-557(1986).
RN [6]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [7]
RP CHARACTERIZATION.
RX PubMed=10497017; DOI=10.1006/jmbi.1999.3088;
RA Wang W.-F., Margolin W., Molineux I.J.;
RT "Increased synthesis of an Escherichia coli membrane protein suppresses F
RT exclusion of bacteriophage T7.";
RL J. Mol. Biol. 292:501-512(1999).
RN [8]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Overexpression alleviates the exclusion of phage T7 in cells
CC harboring the F plasmid.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the UPF0716 (FxsA) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA97039.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA27700.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U78484; AAB36686.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97039.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC77100.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE78142.1; -; Genomic_DNA.
DR EMBL; X04066; CAA27700.1; ALT_INIT; Genomic_DNA.
DR PIR; S56368; S56368.
DR RefSeq; NP_418564.2; NC_000913.3.
DR RefSeq; WP_001267448.1; NZ_STEB01000014.1.
DR AlphaFoldDB; P37147; -.
DR BioGRID; 4262196; 13.
DR IntAct; P37147; 3.
DR STRING; 511145.b4140; -.
DR jPOST; P37147; -.
DR PaxDb; P37147; -.
DR PRIDE; P37147; -.
DR EnsemblBacteria; AAC77100; AAC77100; b4140.
DR EnsemblBacteria; BAE78142; BAE78142; BAE78142.
DR GeneID; 66671948; -.
DR GeneID; 948657; -.
DR KEGG; ecj:JW4100; -.
DR KEGG; eco:b4140; -.
DR PATRIC; fig|1411691.4.peg.2560; -.
DR EchoBASE; EB2284; -.
DR eggNOG; COG3030; Bacteria.
DR HOGENOM; CLU_085083_0_0_6; -.
DR InParanoid; P37147; -.
DR OMA; WLRGMSK; -.
DR PhylomeDB; P37147; -.
DR BioCyc; EcoCyc:G7832-MON; -.
DR PRO; PR:P37147; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IDA:EcoCyc.
DR GO; GO:0031226; C:intrinsic component of plasma membrane; IDA:EcoliWiki.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR InterPro; IPR007313; FxsA.
DR PANTHER; PTHR35335; PTHR35335; 1.
DR Pfam; PF04186; FxsA; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..158
FT /note="UPF0716 protein FxsA"
FT /id="PRO_0000087394"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..78
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 125..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 158 AA; 17692 MW; DB2338BFE146076D CRC64;
MRWLPFIAIF LYVYIEISIF IQVAHVLGVL LTLVLVIFTS VIGMSLVRNQ GFKNFVLMQQ
KMAAGENPAA EMIKSVSLII AGLLLLLPGF FTDFLGLLLL LPPVQKHLTV KLMPHLRFSR
MPGGGFSAGT GGGNTFDGEY QRKDDERDRL DHKDDRQD
