ID   FXYD3_PIG               Reviewed;          88 AA.
AC   O97797;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=FXYD domain-containing ion transport regulator 3;
DE   AltName: Full=Chloride conductance inducer protein Mat-8;
DE   AltName: Full=Mammary tumor 8 kDa protein;
DE   AltName: Full=Sodium/potassium-transporting ATPase subunit FXYD3 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=FXYD3; Synonyms=MAT8;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Gastric mucosa;
RX   PubMed=10206733; DOI=10.1247/csf.23.315;
RA   Maeda M., Hamano K., Hirano Y., Suzuki M., Takahashi E., Terada T.,
RA   Futai M., Sato R.;
RT   "Structures of P-type transporting ATPases and chromosomal locations of
RT   their genes.";
RL   Cell Struct. Funct. 23:315-323(1998).
CC   -!- FUNCTION: Associates with and regulates the activity of the
CC       sodium/potassium-transporting ATPase (NKA) which transports Na(+) out
CC       of the cell and K(+) into the cell. Reduces glutathionylation of the
CC       NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated
CC       inhibition of ATP1B1. Induces a hyperpolarization-activated chloride
CC       current when expressed in Xenopus oocytes.
CC       {ECO:0000250|UniProtKB:Q14802}.
CC   -!- SUBUNIT: Regulatory subunit of the sodium/potassium-transporting ATPase
CC       which is composed of a catalytic alpha subunit, a non-catalytic beta
CC       subunit and an additional regulatory subunit. Interacts with catalytic
CC       alpha subunit ATP1A1. Also interacts with non-catalytic beta subunit
CC       ATP1B1. Interacts with the alpha1-beta1, alpha2-beta1 and alpha3-beta1
CC       NKA isozymes. {ECO:0000250|UniProtKB:Q14802,
CC       ECO:0000250|UniProtKB:Q61835}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000255}.
CC   -!- PTM: Glutathionylated. {ECO:0000250|UniProtKB:Q14802}.
CC   -!- SIMILARITY: Belongs to the FXYD family. {ECO:0000305}.
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DR   EMBL; AB015759; BAA35078.1; -; mRNA.
DR   RefSeq; NP_999373.1; NM_214208.2.
DR   AlphaFoldDB; O97797; -.
DR   SMR; O97797; -.
DR   STRING; 9823.ENSSSCP00000023061; -.
DR   PaxDb; O97797; -.
DR   GeneID; 397413; -.
DR   KEGG; ssc:397413; -.
DR   CTD; 5349; -.
DR   eggNOG; ENOG502S9Z9; Eukaryota.
DR   InParanoid; O97797; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0043269; P:regulation of ion transport; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000272; Ion-transport_regulator_FXYD.
DR   Pfam; PF02038; ATP1G1_PLM_MAT8; 1.
DR   PROSITE; PS01310; FXYD; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Glutathionylation; Ion transport; Membrane; Potassium;
KW   Potassium transport; Reference proteome; Signal; Sodium; Sodium transport;
KW   Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000250|UniProtKB:Q14802"
FT   CHAIN           18..88
FT                   /note="FXYD domain-containing ion transport regulator 3"
FT                   /id="PRO_0000010364"
FT   TOPO_DOM        18..38
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        60..88
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          66..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   88 AA;  9314 MW;  6CC7810B90512E5A CRC64;
     MHEVALSVLI LLAGLSALDA NDPEDKNSPF YYDWHSLRVG GLICAGTPCA LGIIILLSGK
     CKCKFSQKPS HRPGDAPPLI TPGSAHDC