ID   FXYD3_RAT               Reviewed;          88 AA.
AC   P59645;
DT   30-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-APR-2003, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=FXYD domain-containing ion transport regulator 3;
DE   AltName: Full=Sodium/potassium-transporting ATPase subunit FXYD3 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=Fxyd3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Lee N.H., Glodek A., Chandra I., Mason T.M., Quackenbush J.,
RA   Kerlavage A.R., Adams M.D.;
RL   Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   RECONSTRUCTION FROM ESTS, AND CONCEPTUAL TRANSLATION.
RX   PubMed=10950925; DOI=10.1006/geno.2000.6274;
RA   Sweadner K.J., Rael E.;
RT   "The FXYD gene family of small ion transport regulators or channels: cDNA
RT   sequence, protein signature sequence, and expression.";
RL   Genomics 68:41-56(2000).
CC   -!- FUNCTION: Associates with and regulates the activity of the
CC       sodium/potassium-transporting ATPase (NKA) which transports Na(+) out
CC       of the cell and K(+) into the cell. Reduces glutathionylation of the
CC       NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated
CC       inhibition of ATP1B1. Induces a hyperpolarization-activated chloride
CC       current when expressed in Xenopus oocytes.
CC       {ECO:0000250|UniProtKB:Q14802}.
CC   -!- SUBUNIT: Regulatory subunit of the sodium/potassium-transporting ATPase
CC       which is composed of a catalytic alpha subunit, a non-catalytic beta
CC       subunit and an additional regulatory subunit. Interacts with catalytic
CC       alpha subunit ATP1A1. Also interacts with non-catalytic beta subunit
CC       ATP1B1. Interacts with the alpha1-beta1, alpha2-beta1 and alpha3-beta1
CC       NKA isozymes. {ECO:0000250|UniProtKB:Q14802,
CC       ECO:0000250|UniProtKB:Q61835}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000255}.
CC   -!- PTM: Glutathionylated. {ECO:0000250|UniProtKB:Q14802}.
CC   -!- SIMILARITY: Belongs to the FXYD family. {ECO:0000305}.
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DR   EMBL; AA801365; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_758528.1; NM_172317.2.
DR   AlphaFoldDB; P59645; -.
DR   SMR; P59645; -.
DR   STRING; 10116.ENSRNOP00000028637; -.
DR   PhosphoSitePlus; P59645; -.
DR   PaxDb; P59645; -.
DR   GeneID; 116831; -.
DR   KEGG; rno:116831; -.
DR   UCSC; RGD:620825; rat.
DR   CTD; 5349; -.
DR   RGD; 620825; Fxyd3.
DR   eggNOG; ENOG502S9Z9; Eukaryota.
DR   HOGENOM; CLU_171208_0_1_1; -.
DR   InParanoid; P59645; -.
DR   OMA; IVLMSEW; -.
DR   OrthoDB; 1606794at2759; -.
DR   PhylomeDB; P59645; -.
DR   TreeFam; TF333443; -.
DR   Reactome; R-RNO-5578775; Ion homeostasis.
DR   Reactome; R-RNO-936837; Ion transport by P-type ATPases.
DR   PRO; PR:P59645; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000021095; Expressed in stomach and 18 other tissues.
DR   Genevisible; P59645; RN.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:RGD.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051117; F:ATPase binding; ISO:RGD.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:InterPro.
DR   GO; GO:0006811; P:ion transport; NAS:RGD.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0050790; P:regulation of catalytic activity; ISO:RGD.
DR   GO; GO:0043269; P:regulation of ion transport; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000272; Ion-transport_regulator_FXYD.
DR   Pfam; PF02038; ATP1G1_PLM_MAT8; 1.
DR   PROSITE; PS01310; FXYD; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Glutathionylation; Ion transport; Membrane; Potassium;
KW   Potassium transport; Reference proteome; Signal; Sodium; Sodium transport;
KW   Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000250|UniProtKB:Q14802"
FT   CHAIN           21..88
FT                   /note="FXYD domain-containing ion transport regulator 3"
FT                   /id="PRO_0000010365"
FT   TOPO_DOM        21..38
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        60..88
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          66..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   88 AA;  9411 MW;  45AFE872FD1AF944 CRC64;
     MQEFALSLLV LLAGLPTLDA NDPEDKDSPF YYDWHSLRVG GLICAGILCA LGIIVLMSGK
     CKCKFSQKPS HRPGDGPPLI TPGSAHNC