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FYN_BOVIN
ID   FYN_BOVIN               Reviewed;         537 AA.
AC   A0JNB0;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Tyrosine-protein kinase Fyn;
DE            EC=2.7.10.2;
DE   AltName: Full=Proto-oncogene c-Fyn;
DE   AltName: Full=p59-Fyn;
GN   Name=FYN {ECO:0000312|EMBL:AAI26592.1};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1] {ECO:0000312|EMBL:AAI26592.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford {ECO:0000312|EMBL:AAI26592.1};
RC   TISSUE=Fetal medulla {ECO:0000312|EMBL:AAI26592.1};
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=10930415; DOI=10.1074/jbc.m005275200;
RA   Edwards J.C., Kapadia S.;
RT   "Regulation of the bovine kidney microsomal chloride channel p64 by p59fyn,
RT   a Src family tyrosine kinase.";
RL   J. Biol. Chem. 275:31826-31832(2000).
CC   -!- FUNCTION: Non-receptor tyrosine-protein kinase that plays a role in
CC       many biological processes including regulation of cell growth and
CC       survival, cell adhesion, integrin-mediated signaling, cytoskeletal
CC       remodeling, cell motility, immune response and axon guidance. Inactive
CC       FYN is phosphorylated on its C-terminal tail within the catalytic
CC       domain. Following activation by PKA, the protein subsequently
CC       associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation,
CC       activation and targeting to focal adhesions. Involved in the regulation
CC       of cell adhesion and motility through phosphorylation of CTNNB1 (beta-
CC       catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling
CC       by phosphorylating several proteins including the actin regulator WAS
CC       and the microtubule-associated proteins MAP2 and MAPT. Promotes cell
CC       survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic
CC       cleavage. Participates in signal transduction pathways that regulate
CC       the integrity of the glomerular slit diaphragm (an essential part of
CC       the glomerular filter of the kidney) by phosphorylating several slit
CC       diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role
CC       in neural processes by phosphorylating DPYSL2, a multifunctional
CC       adapter protein within the central nervous system, ARHGAP32, a
CC       regulator for Rho family GTPases implicated in various neural
CC       functions, and SNCA, a small pre-synaptic protein. Participates in the
CC       downstream signaling pathways that lead to T-cell differentiation and
CC       proliferation following T-cell receptor (TCR) stimulation.
CC       Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation.
CC       Also participates in negative feedback regulation of TCR signaling
CC       through phosphorylation of PAG1, thereby promoting interaction between
CC       PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK
CC       and FYN in an inactive form. Promotes CD28-induced phosphorylation of
CC       VAV1. In mast cells, phosphorylates CLNK after activation of
CC       immunoglobulin epsilon receptor signaling (By similarity).
CC       {ECO:0000250|UniProtKB:P06241, ECO:0000250|UniProtKB:P39688,
CC       ECO:0000269|PubMed:10930415}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC         [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC         COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC         ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10028,
CC         ECO:0000269|PubMed:10930415};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: Inhibited by phosphorylation of Tyr-531 by
CC       leukocyte common antigen and activated by dephosphorylation of this
CC       site. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via its SH3 domain) with PIK3R1 and PRMT8.
CC       Interacts with FYB1, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-
CC       phosphorylated form); the interaction increases FYN activity. Interacts
CC       (via SH2 domain) with CSF1R (tyrosine phosphorylated) (By similarity).
CC       Interacts with TOM1L1 (phosphorylated form). Interacts with KDR
CC       (tyrosine phosphorylated). Interacts (via SH3 domain) with KLHL2 (via
CC       N-terminus) (By similarity). Interacts with SH2D1A and SLAMF1.
CC       Interacts with ITCH; the interaction phosphorylates ITCH and negatively
CC       regulates its activity. Interacts with FASLG. Interacts with RUNX3.
CC       Interacts with KIT. Interacts with EPHA8; possible downstream effector
CC       of EPHA8 in regulation of cell adhesion. Interacts with PTK2/FAK1; this
CC       interaction leads to PTK2/FAK1 phosphorylation and activation.
CC       Interacts with CAV1; this interaction couples integrins to the Ras-ERK
CC       pathway. Interacts with UNC119. Interacts (via SH2 domain) with PTPRH
CC       (phosphorylated form) (By similarity). Interacts with PTPRO
CC       (phosphorylated form) (By similarity). Interacts with PTPRB
CC       (phosphorylated form) (By similarity). Interacts with FYB2 (By
CC       similarity). Interacts with DSCAM (By similarity). Interacts with SKAP1
CC       and FYB1; this interaction promotes the phosphorylation of CLNK (By
CC       similarity). {ECO:0000250|UniProtKB:P06241,
CC       ECO:0000250|UniProtKB:P39688, ECO:0000250|UniProtKB:Q62844}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC       Cell membrane {ECO:0000250}. Note=Present and active in lipid rafts.
CC       Palmitoylation is crucial for proper trafficking (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on
CC       the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an
CC       inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to
CC       activation. Ultraviolet B (UVB) strongly increase phosphorylation at
CC       Thr-12 and kinase activity, and promotes translocation from the
CC       cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2
CC       negatively regulates T-cell receptor signaling (By similarity).
CC       Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By
CC       similarity). {ECO:0000250|UniProtKB:P06241,
CC       ECO:0000250|UniProtKB:P39688}.
CC   -!- PTM: Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by
CC       ZDHHC21, regulates subcellular location.
CC       {ECO:0000250|UniProtKB:P39688}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein
CC       kinase family. SRC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR   EMBL; BC126591; AAI26592.1; -; mRNA.
DR   RefSeq; NP_001071440.1; NM_001077972.1.
DR   RefSeq; XP_005210845.1; XM_005210788.2.
DR   RefSeq; XP_005210846.1; XM_005210789.3.
DR   RefSeq; XP_005210847.1; XM_005210790.3.
DR   AlphaFoldDB; A0JNB0; -.
DR   BMRB; A0JNB0; -.
DR   SMR; A0JNB0; -.
DR   STRING; 9913.ENSBTAP00000015730; -.
DR   PaxDb; A0JNB0; -.
DR   PRIDE; A0JNB0; -.
DR   Ensembl; ENSBTAT00000015730; ENSBTAP00000015730; ENSBTAG00000011851.
DR   GeneID; 527263; -.
DR   KEGG; bta:527263; -.
DR   CTD; 2534; -.
DR   VEuPathDB; HostDB:ENSBTAG00000011851; -.
DR   VGNC; VGNC:29163; FYN.
DR   eggNOG; KOG0197; Eukaryota.
DR   GeneTree; ENSGT00940000155462; -.
DR   HOGENOM; CLU_000288_7_2_1; -.
DR   InParanoid; A0JNB0; -.
DR   OMA; WMEKADG; -.
DR   OrthoDB; 539311at2759; -.
DR   TreeFam; TF351634; -.
DR   Reactome; R-BTA-114604; GPVI-mediated activation cascade.
DR   Reactome; R-BTA-1227986; Signaling by ERBB2.
DR   Reactome; R-BTA-1257604; PIP3 activates AKT signaling.
DR   Reactome; R-BTA-1433557; Signaling by SCF-KIT.
DR   Reactome; R-BTA-1433559; Regulation of KIT signaling.
DR   Reactome; R-BTA-210990; PECAM1 interactions.
DR   Reactome; R-BTA-2424491; DAP12 signaling.
DR   Reactome; R-BTA-375165; NCAM signaling for neurite out-growth.
DR   Reactome; R-BTA-389356; CD28 co-stimulation.
DR   Reactome; R-BTA-389357; CD28 dependent PI3K/Akt signaling.
DR   Reactome; R-BTA-389359; CD28 dependent Vav1 pathway.
DR   Reactome; R-BTA-389513; CTLA4 inhibitory signaling.
DR   Reactome; R-BTA-3928663; EPHA-mediated growth cone collapse.
DR   Reactome; R-BTA-3928664; Ephrin signaling.
DR   Reactome; R-BTA-3928665; EPH-ephrin mediated repulsion of cells.
DR   Reactome; R-BTA-399954; Sema3A PAK dependent Axon repulsion.
DR   Reactome; R-BTA-399956; CRMPs in Sema3A signaling.
DR   Reactome; R-BTA-4420097; VEGFA-VEGFR2 Pathway.
DR   Reactome; R-BTA-5621480; Dectin-2 family.
DR   Reactome; R-BTA-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-BTA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR   Reactome; R-BTA-75892; Platelet Adhesion to exposed collagen.
DR   Reactome; R-BTA-912631; Regulation of signaling by CBL.
DR   Proteomes; UP000009136; Chromosome 9.
DR   Bgee; ENSBTAG00000011851; Expressed in floor plate of diencephalon and 105 other tissues.
DR   GO; GO:0005884; C:actin filament; IEA:Ensembl.
DR   GO; GO:0044297; C:cell body; IEA:Ensembl.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0030425; C:dendrite; IEA:Ensembl.
DR   GO; GO:0005768; C:endosome; IEA:Ensembl.
DR   GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IBA:GO_Central.
DR   GO; GO:0097386; C:glial cell projection; IEA:Ensembl.
DR   GO; GO:0045121; C:membrane raft; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0014069; C:postsynaptic density; IEA:Ensembl.
DR   GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IEA:Ensembl.
DR   GO; GO:0043014; F:alpha-tubulin binding; IEA:Ensembl.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0097718; F:disordered domain specific binding; IEA:Ensembl.
DR   GO; GO:0046875; F:ephrin receptor binding; IEA:Ensembl.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IEA:Ensembl.
DR   GO; GO:0070851; F:growth factor receptor binding; IEA:Ensembl.
DR   GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IBA:GO_Central.
DR   GO; GO:0016004; F:phospholipase activator activity; IEA:Ensembl.
DR   GO; GO:0043274; F:phospholipase binding; IEA:Ensembl.
DR   GO; GO:0004713; F:protein tyrosine kinase activity; ISS:UniProtKB.
DR   GO; GO:0097110; F:scaffold protein binding; IEA:Ensembl.
DR   GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR   GO; GO:0048156; F:tau protein binding; IEA:Ensembl.
DR   GO; GO:0044325; F:transmembrane transporter binding; IEA:Ensembl.
DR   GO; GO:0050798; P:activated T cell proliferation; IEA:Ensembl.
DR   GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:1904646; P:cellular response to amyloid-beta; IEA:Ensembl.
DR   GO; GO:0036120; P:cellular response to platelet-derived growth factor stimulus; IEA:Ensembl.
DR   GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEA:Ensembl.
DR   GO; GO:0048813; P:dendrite morphogenesis; IEA:Ensembl.
DR   GO; GO:0050966; P:detection of mechanical stimulus involved in sensory perception of pain; IEA:Ensembl.
DR   GO; GO:0030900; P:forebrain development; IEA:Ensembl.
DR   GO; GO:0003015; P:heart process; IEA:Ensembl.
DR   GO; GO:0007249; P:I-kappaB kinase/NF-kappaB signaling; IEA:Ensembl.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   GO; GO:0050804; P:modulation of chemical synaptic transmission; IEA:Ensembl.
DR   GO; GO:1902951; P:negative regulation of dendritic spine maintenance; IEA:Ensembl.
DR   GO; GO:0010629; P:negative regulation of gene expression; IEA:Ensembl.
DR   GO; GO:0042177; P:negative regulation of protein catabolic process; IEA:Ensembl.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; IEA:Ensembl.
DR   GO; GO:0001764; P:neuron migration; IEA:Ensembl.
DR   GO; GO:0018108; P:peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
DR   GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IEA:Ensembl.
DR   GO; GO:1901216; P:positive regulation of neuron death; IEA:Ensembl.
DR   GO; GO:0010976; P:positive regulation of neuron projection development; IEA:Ensembl.
DR   GO; GO:1900182; P:positive regulation of protein localization to nucleus; IEA:Ensembl.
DR   GO; GO:0090314; P:positive regulation of protein targeting to membrane; IEA:Ensembl.
DR   GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl.
DR   GO; GO:0046777; P:protein autophosphorylation; IEA:Ensembl.
DR   GO; GO:0030163; P:protein catabolic process; IEA:Ensembl.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:Ensembl.
DR   GO; GO:1905664; P:regulation of calcium ion import across plasma membrane; IEA:Ensembl.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:Ensembl.
DR   GO; GO:1900449; P:regulation of glutamate receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR   GO; GO:0050852; P:T cell receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IBA:GO_Central.
DR   CDD; cd12006; SH3_Fyn_Yrk; 1.
DR   Gene3D; 3.30.505.10; -; 1.
DR   InterPro; IPR035750; Fyn/Yrk_SH3.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR   InterPro; IPR000980; SH2.
DR   InterPro; IPR036860; SH2_dom_sf.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   InterPro; IPR008266; Tyr_kinase_AS.
DR   InterPro; IPR020635; Tyr_kinase_cat_dom.
DR   Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR   Pfam; PF00017; SH2; 1.
DR   Pfam; PF00018; SH3_1; 1.
DR   PRINTS; PR00401; SH2DOMAIN.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   PRINTS; PR00109; TYRKINASE.
DR   SMART; SM00252; SH2; 1.
DR   SMART; SM00326; SH3; 1.
DR   SMART; SM00219; TyrKc; 1.
DR   SUPFAM; SSF50044; SSF50044; 1.
DR   SUPFAM; SSF55550; SSF55550; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR   PROSITE; PS50001; SH2; 1.
DR   PROSITE; PS50002; SH3; 1.
PE   2: Evidence at transcript level;
KW   Adaptive immunity; ATP-binding; Cell membrane; Cytoplasm;
KW   Developmental protein; Immunity; Kinase; Lipoprotein; Manganese; Membrane;
KW   Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate;
KW   Phosphoprotein; Proto-oncogene; Reference proteome; SH2 domain; SH3 domain;
KW   Transferase; Tyrosine-protein kinase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P06241"
FT   CHAIN           2..537
FT                   /note="Tyrosine-protein kinase Fyn"
FT                   /id="PRO_0000282314"
FT   DOMAIN          82..143
FT                   /note="SH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          149..246
FT                   /note="SH2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT   DOMAIN          271..524
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          14..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..35
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        390
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P28523,
FT                   ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-
FT                   ProRule:PRU10028"
FT   BINDING         277..285
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P28523,
FT                   ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         299
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q62844,
FT                   ECO:0000255|PROSITE-ProRule:PRU00159"
FT   MOD_RES         12
FT                   /note="Phosphothreonine; by PKC"
FT                   /evidence="ECO:0000250|UniProtKB:P06241"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P06241"
FT   MOD_RES         26
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P06241"
FT   MOD_RES         185
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P39688"
FT   MOD_RES         420
FT                   /note="Phosphotyrosine; by autocatalysis"
FT                   /evidence="ECO:0000250|UniProtKB:P39688"
FT   MOD_RES         531
FT                   /note="Phosphotyrosine; by CSK"
FT                   /evidence="ECO:0000250|UniProtKB:P06241"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000250|UniProtKB:P39688"
FT   LIPID           3
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000250|UniProtKB:P39688"
FT   LIPID           6
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000250|UniProtKB:P39688"
SQ   SEQUENCE   537 AA;  60718 MW;  A048E0F5193D7CFF CRC64;
     MGCVQCKDKE ATKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY NNFHGAGGQG
     LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFHKGEKFQ ILNSSEGDWW
     EARSLTTGET GYIPSNYVAP VDSIQAEEWY FGKLGRKDAE RQLLSFGNPR GTFLIRESET
     TKGAYSLSIR DWDDMKGDHV KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC
     RLVVPCHKGM PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT
     LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR
     ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN GLICKIADFG LARLIEDNEY
     TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE
     RGYRMPCPQD CPISLHELMI HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL
 
 
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