FZD3_MOUSE
ID FZD3_MOUSE Reviewed; 666 AA.
AC Q61086;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Frizzled-3;
DE Short=Fz-3;
DE Short=mFz3;
DE Flags: Precursor;
GN Name=Fzd3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=8626800; DOI=10.1074/jbc.271.8.4468;
RA Wang Y., Macke J.P., Abella B.S., Andreasson K., Worley P., Gilbert D.J.,
RA Copeland N.G., Jenkins N.A., Nathans J.;
RT "A large family of putative transmembrane receptors homologous to the
RT product of the Drosophila tissue polarity gene frizzled.";
RL J. Biol. Chem. 271:4468-4476(1996).
RN [2]
RP WNT-MEDIATED PKC ACTIVATION.
RX PubMed=10395542; DOI=10.1016/s0960-9822(99)80310-8;
RA Sheldahl L.C., Park M., Malbon C.C., Moon R.T.;
RT "Protein kinase C is differentially stimulated by Wnt and Frizzled homologs
RT in a G-protein-dependent manner.";
RL Curr. Biol. 9:695-698(1999).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11407985; DOI=10.1046/j.1523-1747.2001.01336.x;
RA Hung B.S., Wang X.-Q., Cam G.R., Rothnagel J.A.;
RT "Characterization of mouse frizzled-3 expression in hair follicle
RT development and identification of the human homolog in keratinocytes.";
RL J. Invest. Dermatol. 116:940-946(2001).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=12351730; DOI=10.1523/jneurosci.22-19-08563.2002;
RA Wang Y., Thekdi N., Smallwood P.M., Macke J.P., Nathans J.;
RT "Frizzled-3 is required for the development of major fiber tracts in the
RT rostral CNS.";
RL J. Neurosci. 22:8563-8573(2002).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=14671310; DOI=10.1126/science.1089610;
RA Lyuksyutova A.I., Lu C.C., Milanesio N., King L.A., Gu o N., Wang Y.,
RA Nathans J., Tessier-Lavigne M., Zou Y.;
RT "Anterior-posterior guidance of commissural axons by Wnt-frizzled
RT signaling.";
RL Science 302:1984-1988(2003).
RN [6]
RP FUNCTION.
RX PubMed=16407530; DOI=10.1523/jneurosci.3221-05.2006;
RA Wang Y., Zhang J., Mori S., Nathans J.;
RT "Axonal growth and guidance defects in Frizzled3 knock-out mice: a
RT comparison of diffusion tensor magnetic resonance imaging, neurofilament
RT staining, and genetically directed cell labeling.";
RL J. Neurosci. 26:355-364(2006).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=16495441; DOI=10.1523/jneurosci.4698-05.2005;
RA Wang Y., Guo N., Nathans J.;
RT "The role of Frizzled3 and Frizzled6 in neural tube closure and in the
RT planar polarity of inner-ear sensory hair cells.";
RL J. Neurosci. 26:2147-2156(2006).
RN [8]
RP INTERACTION WITH VANGL2.
RX PubMed=16687519; DOI=10.1523/jneurosci.4680-05.2006;
RA Montcouquiol M., Sans N., Huss D., Kach J., Dickman J.D., Forge A.,
RA Rachel R.A., Copeland N.G., Jenkins N.A., Bogani D., Murdoch J.,
RA Warchol M.E., Wenthold R.J., Kelley M.W.;
RT "Asymmetric localization of Vangl2 and Fz3 indicate novel mechanisms for
RT planar cell polarity in mammals.";
RL J. Neurosci. 26:5265-5275(2006).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=21325504; DOI=10.1523/jneurosci.4243-10.2011;
RA Armstrong A., Ryu Y.K., Chieco D., Kuruvilla R.;
RT "Frizzled3 is required for neurogenesis and target innervation during
RT sympathetic nervous system development.";
RL J. Neurosci. 31:2371-2381(2011).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND CONDITIONAL KNOCKOUTS.
RX PubMed=24347548; DOI=10.7554/elife.01482;
RA Hua Z.L., Smallwood P.M., Nathans J.;
RT "Frizzled3 controls axonal development in distinct populations of cranial
RT and spinal motor neurons.";
RL Elife 2:E01482-E01482(2013).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, AND CONDITIONAL KNOCKOUTS.
RX PubMed=24799694; DOI=10.1073/pnas.1406399111;
RA Hua Z.L., Jeon S., Caterina M.J., Nathans J.;
RT "Frizzled3 is required for the development of multiple axon tracts in the
RT mouse central nervous system.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:E3005-E3005(2014).
CC -!- FUNCTION: Receptor for Wnt proteins. Most of frizzled receptors are
CC coupled to the beta-catenin canonical signaling pathway, which leads to
CC the activation of disheveled proteins, inhibition of GSK-3 kinase,
CC nuclear accumulation of beta-catenin and activation of Wnt target
CC genes. A second signaling pathway involving PKC and calcium fluxes has
CC been seen for some family members, but it is not yet clear if it
CC represents a distinct pathway or if it can be integrated in the
CC canonical pathway, as PKC seems to be required for Wnt-mediated
CC inactivation of GSK-3 kinase. Both pathways seem to involve
CC interactions with G-proteins. Activation by Wnt5A stimulates PKC
CC activity via a G-protein-dependent mechanism. Involved in transduction
CC and intercellular transmission of polarity information during tissue
CC morphogenesis and/or in differentiated tissues. Plays a role in
CC controlling early axon growth and guidance processes necessary for the
CC formation of a subset of central and peripheral major fiber tracts.
CC Required for the development of major fiber tracts in the central
CC nervous system, including: the anterior commissure, the corpus
CC callosum, the thalamocortical, corticothalamic and nigrostriatal
CC tracts, the corticospinal tract, the fasciculus retroflexus, the
CC mammillothalamic tract, the medial lemniscus, and ascending fiber
CC tracts from the spinal cord to the brain. In the peripheral nervous
CC system, controls axon growth in distinct populations of cranial and
CC spinal motor neurons, including the facial branchimotor nerve, the
CC hypoglossal nerve, the phrenic nerve, and motor nerves innervating
CC dorsal limbs. Involved in the migration of cranial neural crest cells.
CC May also be implicated in the transmission of sensory information from
CC the trunk and limbs to the brain. Controls commissural sensory axons
CC guidance after midline crossing along the anterior-posterior axis in
CC the developing spinal cord in a Wnt-dependent signaling pathway.
CC Together with FZD6, is involved in the neural tube closure and plays a
CC role in the regulation of the establishment of planar cell polarity
CC (PCP), particularly in the orientation of asymmetric bundles of
CC stereocilia on the apical faces of a subset of auditory and vestibular
CC sensory cells located in the inner ear. Promotes neurogenesis by
CC maintaining sympathetic neuroblasts within the cell cycle in a beta-
CC catenin-dependent manner. {ECO:0000269|PubMed:12351730,
CC ECO:0000269|PubMed:14671310, ECO:0000269|PubMed:16407530,
CC ECO:0000269|PubMed:16495441, ECO:0000269|PubMed:21325504,
CC ECO:0000269|PubMed:24347548, ECO:0000269|PubMed:24799694}.
CC -!- SUBUNIT: Interacts with VANGL2. {ECO:0000269|PubMed:16687519}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cell
CC surface {ECO:0000269|PubMed:16495441}. Apical cell membrane
CC {ECO:0000269|PubMed:16495441}; Multi-pass membrane protein.
CC Note=Colocalizes with FZD6 at the apical face of the cell.
CC {ECO:0000269|PubMed:16495441}.
CC -!- TISSUE SPECIFICITY: Expressed in the cortex, diencephalon, rostral
CC brainstem and little or no staining is seen in the striatum or
CC cerebellum. Expressed in both hair cells and supporting cells in the
CC utricle, saccule, cristae and the organ of Corti in the inner ear (at
CC protein level). Highly expressed in the CNS. In skin, it is restricted
CC to the epidermis and to the developing hair follicle.
CC {ECO:0000269|PubMed:11407985, ECO:0000269|PubMed:12351730,
CC ECO:0000269|PubMed:16495441, ECO:0000269|PubMed:8626800}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout the developing central
CC nervous system (CNS). Expressed in the cortex, diencephalon, and
CC brainstem, with the most intense staining in the striatum and
CC trigeminal ganglia at 18 dpc (at protein level). First detected in
CC discrete foci in the developing epidermis of 13 days old embryos, later
CC in the hair follicle placodes of 15 days old embryos. Expressed in the
CC ventral and lateral margins of the spinal cord from 9.5 to 13.5 dpc,
CC where post-crossing commissural axons project longitudinally. Expressed
CC in superior sympathetic cervical ganglia (SCG) at 14.5 and 16.5 dpc, a
CC stage when the SCG is comprised primarily of proliferating sympathetic
CC neuroblasts. In 17 days embryos and 1 day old newborn, expression is
CC limited to suprabasal keratinocytes and is not seen in pelage follicles
CC until 3 days postpartum. In 7 days old neonatal skin, expression occurs
CC throughout the epidermis and in the outer cell layers of hair
CC follicles. {ECO:0000269|PubMed:11407985, ECO:0000269|PubMed:12351730,
CC ECO:0000269|PubMed:14671310, ECO:0000269|PubMed:21325504}.
CC -!- DOMAIN: Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl
CC (Disheveled) family members and is involved in the activation of the
CC Wnt/beta-catenin signaling pathway. {ECO:0000250}.
CC -!- DOMAIN: The FZ domain is involved in binding with Wnt ligands.
CC {ECO:0000250}.
CC -!- PTM: Ubiquitinated by ZNRF3, leading to its degradation by the
CC proteasome. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Neonate knockout mice have a curly tail, flexed
CC lower limbs, breathe irregularly and typically die within 30 min of
CC birth. Central nervous system (CNS) shows severe defects in the
CC development of several major axon tracts, including: a nearly complete
CC absence of the three early most prominent axon tracts in the brain and
CC the ventral branch of the trigeminal nerve, absence of subcortical and
CC striatal axons, the anterior commissure, misrouting of thalamocortical
CC axons, a nearly complete absence of the corticospinal tract, the
CC fasciculus retroflexus, and the mammillothalamic tract, poor
CC fasciculation of the medial lemniscus and a disorganization of axon
CC bundles in the reticular formation, severe defect in the asymmetric
CC rostrocaudal orientation of dopaminergic and serotonergic axons, a
CC large reduction or complete absence of ascending spinal axon tracts in
CC the braistem, midbrain and thalamus, peripheral nerves defect in
CC several motor neurons, such as in the VIIth and XIIth cranial motor
CC nerves, the phrenic nerve, and the spinal motor nerve which failed to
CC form connections with their respective targets and display also
CC aberrant migration of a subpopulation of cranial neural crest cells
CC (PubMed:12351730, PubMed:24347548, PubMed:24799694). Neonate knockout
CC mice show fewer S-phase proliferating neuroblasts, premature cell cycle
CC exit and enhanced apoptosis in early-stage superior cervical ganglia
CC (SCGs), and in some cases, complete absence of sympathetic innervation
CC of several peripheral targets (PubMed:21325504). Display also impaired
CC rostral turning by growth cones of spinal cord commissural sensory
CC axons (PubMed:14671310). FZD3 and FZD6 double knockout embryos have a
CC curled tail, exhibit defects in neural tube and eyelids closure, in the
CC orientation of hair bundles on inner-ear sensory cells and die at birth
CC (PubMed:16495441). The following conditional knockout mice display the
CC corresponding phenotypes: dopaminergic neuron-specific shows a defect
CC in the orientation and growth of midbrain dopaminergic axons with an
CC absence of striatum innervation; retinal ganglion cell (RGC)-specific
CC displays a misrouting of a subset of optic tract axons and a lack of
CC the medial terminal nucleus (MTN) innervation; neocortex neuron-
CC specific displays a total absence of the posterior part of the anterior
CC commissure and aberrant axon trajectories appearing in the external
CC capsule; ventral telencephalon neuron-specific shows corticothalamic,
CC thalamocortical and corticospinal tracts defect to various extent;
CC telencephalon neuron-specific exhibits the full spectrum of axon
CC defects seen in the classical null mutant knockout mice; cholinergic
CC neuron-specific shows an absence of cholinergic fiber tracts passing
CC through the striatum, a defective caudal migration of neurons of the
CC VIIth motor nucleus and a loss of motor innervation to the face, a
CC decrease in motor innervation of the tongue by the XIIth nerve and a
CC complete loss of cholinergic neurons in the vomeronasal organ;
CC oligodendrocyte neuron-specific leads to the complete spectrum of motor
CC neuron phenotypes shown by the classical mutant knockout mice; caudal
CC and upper thorax region-specific leads to a loss of motor innervation
CC and an atrophy of anterior compartment muscles in the lower hindlimb by
CC the deep peroneal nerve and a nearly absence in ascending spinal
CC sensory axons in the brainstem, midbrain and thalamus altering the
CC ability to transmit sensory information from the trunk and limbs to the
CC brain in postnatal life (PubMed:24347548, PubMed:24799694).
CC {ECO:0000269|PubMed:12351730, ECO:0000269|PubMed:14671310,
CC ECO:0000269|PubMed:16495441, ECO:0000269|PubMed:21325504,
CC ECO:0000269|PubMed:24347548, ECO:0000269|PubMed:24799694}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor Fz/Smo family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U43205; AAC52429.1; -; mRNA.
DR CCDS; CCDS27212.1; -.
DR RefSeq; NP_067433.1; NM_021458.2.
DR AlphaFoldDB; Q61086; -.
DR SMR; Q61086; -.
DR BioGRID; 199776; 1.
DR STRING; 10090.ENSMUSP00000115325; -.
DR GlyGen; Q61086; 3 sites.
DR iPTMnet; Q61086; -.
DR PhosphoSitePlus; Q61086; -.
DR SwissPalm; Q61086; -.
DR MaxQB; Q61086; -.
DR PaxDb; Q61086; -.
DR PRIDE; Q61086; -.
DR ProteomicsDB; 272925; -.
DR Antibodypedia; 10432; 461 antibodies from 35 providers.
DR DNASU; 14365; -.
DR Ensembl; ENSMUST00000131309; ENSMUSP00000115325; ENSMUSG00000007989.
DR GeneID; 14365; -.
DR KEGG; mmu:14365; -.
DR UCSC; uc007ujb.2; mouse.
DR CTD; 7976; -.
DR MGI; MGI:108476; Fzd3.
DR VEuPathDB; HostDB:ENSMUSG00000007989; -.
DR eggNOG; KOG3577; Eukaryota.
DR GeneTree; ENSGT00940000156491; -.
DR HOGENOM; CLU_007873_4_1_1; -.
DR InParanoid; Q61086; -.
DR OMA; WIVFYLW; -.
DR OrthoDB; 330751at2759; -.
DR PhylomeDB; Q61086; -.
DR TreeFam; TF317907; -.
DR Reactome; R-MMU-4086398; Ca2+ pathway.
DR Reactome; R-MMU-4086400; PCP/CE pathway.
DR Reactome; R-MMU-4608870; Asymmetric localization of PCP proteins.
DR BioGRID-ORCS; 14365; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Fzd3; mouse.
DR PRO; PR:Q61086; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q61086; protein.
DR Bgee; ENSMUSG00000007989; Expressed in medial ganglionic eminence and 257 other tissues.
DR Genevisible; Q61086; MM.
DR GO; GO:0045177; C:apical part of cell; IDA:MGI.
DR GO; GO:0016324; C:apical plasma membrane; IDA:MGI.
DR GO; GO:0030424; C:axon; IDA:BHF-UCL.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030425; C:dendrite; IDA:BHF-UCL.
DR GO; GO:0032433; C:filopodium tip; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0016328; C:lateral plasma membrane; IDA:MGI.
DR GO; GO:0043025; C:neuronal cell body; IDA:BHF-UCL.
DR GO; GO:0005886; C:plasma membrane; IDA:BHF-UCL.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0030165; F:PDZ domain binding; ISO:MGI.
DR GO; GO:0042813; F:Wnt receptor activity; IMP:ParkinsonsUK-UCL.
DR GO; GO:0017147; F:Wnt-protein binding; ISO:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; ISO:MGI.
DR GO; GO:0033278; P:cell proliferation in midbrain; IGI:MGI.
DR GO; GO:0071679; P:commissural neuron axon guidance; IMP:MGI.
DR GO; GO:0036514; P:dopaminergic neuron axon guidance; IMP:ParkinsonsUK-UCL.
DR GO; GO:0001736; P:establishment of planar polarity; IGI:MGI.
DR GO; GO:0001942; P:hair follicle development; IEP:BHF-UCL.
DR GO; GO:0042472; P:inner ear morphogenesis; IGI:MGI.
DR GO; GO:0030901; P:midbrain development; IGI:MGI.
DR GO; GO:0097475; P:motor neuron migration; IMP:MGI.
DR GO; GO:1900118; P:negative regulation of execution phase of apoptosis; IMP:UniProtKB.
DR GO; GO:0045976; P:negative regulation of mitotic cell cycle, embryonic; IMP:UniProtKB.
DR GO; GO:0001843; P:neural tube closure; IGI:MGI.
DR GO; GO:1904938; P:planar cell polarity pathway involved in axon guidance; IMP:ParkinsonsUK-UCL.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:0036342; P:post-anal tail morphogenesis; IMP:MGI.
DR GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0036515; P:serotonergic neuron axon guidance; IMP:ParkinsonsUK-UCL.
DR GO; GO:0061549; P:sympathetic ganglion development; IMP:UniProtKB.
DR CDD; cd07449; CRD_FZ3; 1.
DR Gene3D; 1.10.2000.10; -; 1.
DR InterPro; IPR015526; Frizzled/SFRP.
DR InterPro; IPR000539; Frizzled/Smoothened_TM.
DR InterPro; IPR020067; Frizzled_dom.
DR InterPro; IPR036790; Frizzled_dom_sf.
DR InterPro; IPR041769; FZ3_CRD.
DR InterPro; IPR026553; FZD3_vertebrates.
DR InterPro; IPR017981; GPCR_2-like.
DR PANTHER; PTHR11309; PTHR11309; 1.
DR PANTHER; PTHR11309:SF22; PTHR11309:SF22; 1.
DR Pfam; PF01534; Frizzled; 1.
DR Pfam; PF01392; Fz; 1.
DR PRINTS; PR00489; FRIZZLED.
DR SMART; SM00063; FRI; 1.
DR SMART; SM01330; Frizzled; 1.
DR SUPFAM; SSF63501; SSF63501; 1.
DR PROSITE; PS50038; FZ; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Developmental protein; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Neurogenesis; Receptor;
KW Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix;
KW Ubl conjugation; Wnt signaling pathway.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..666
FT /note="Frizzled-3"
FT /id="PRO_0000012983"
FT TOPO_DOM 23..205
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..237
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..288
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..328
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 350..374
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..477
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 499..666
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 23..136
FT /note="FZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT REGION 538..666
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 502..507
FT /note="Lys-Thr-X-X-X-Trp motif, mediates interaction with
FT the PDZ domain of Dvl family members"
FT /evidence="ECO:0000250"
FT COMPBIAS 545..570
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..601
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..635
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 636..666
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 265
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 356
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 28..89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT DISULFID 36..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT DISULFID 73..110
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT DISULFID 99..133
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT DISULFID 103..127
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
SQ SEQUENCE 666 AA; 76208 MW; 0920038B79A73335 CRC64;
MAVSWIVFDL WLLTVFLGQI GGHSLFSCEP ITLRMCQDLP YNTTFMPNLL NHYDQQTAAL
AMEPFHPMVN LDCSRDFRPF LCALYAPICM EYGRVTLPCR RLCQRAYSEC SKLMEMFGVP
WPEDMECSRF PDCDEPYPRL VDLNLVGDPT EGAPVAVQRD YGFWCPRELK IDPDLGYSFL
HVRDCSPPCP NMYFRREELS FARYFIGLIS IICLSATLFT FLTFLIDVTR FRYPERPIIF
YAVCYMMVSL IFFIGFLLED RVACNASSPA QYKASTVTQG SHNKACTMLF MVLYFFTMAG
SVWWVILTIT WFLAAVPKWG SEAIEKKALL FHASAWGIPG TLTIILLAMN KIEGDNISGV
CFVGLYDVDA LRYFVLAPLC LYVVVGVSLL LAGIISLNRV RIEIPLEKEN QDKLVKFMIR
IGVFSILYLV PLLVVIGCYF YEQAYRGIWE TTWIQERCRE YHIPCPYQVT QMSRPDLILF
LMKYLMALIV GIPSIFWVGS KKTCFEWASF FHGRRKKEIV NESRQVLQEP DFAQSLLRDP
NTPIIRKSRG TSTQGTSTHA SSTQLAMVDD QRSKAGSVHS KVSSYHGSLH RSRDGRYTPC
SYRGMEERLP HGSMSRLTDH SRHSSSHRLN EQSRHSSIRD LSNNPMTHIT HGTSMNRVIE
EDGTSA
