FZR2_ARATH
ID FZR2_ARATH Reviewed; 483 AA.
AC Q8L3Z8; O82740;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein FIZZY-RELATED 2;
DE AltName: Full=Cell cycle switch protein CCS52A1;
GN Name=FZR2; Synonyms=CCS52A1, CDH1-2; OrderedLocusNames=At4g22910;
GN ORFNames=F7H19.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP REVIEW.
RX PubMed=12597875; DOI=10.1016/s1360-1385(02)00028-6;
RA Capron A., Okresz L., Genschik P.;
RT "First glance at the plant APC/C, a highly conserved ubiquitin-protein
RT ligase.";
RL Trends Plant Sci. 8:83-89(2003).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, ASSOCIATION WITH THE APC/C COMPLEX, AND
RP INTERACTION WITH CYCA1-1; CYCA1-2; CYCA3-4; CYCB1-1 AND CYCB1-2.
RX PubMed=15970679;
RA Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K.,
RA Bako L., Brown S., Kondorosi A., Kondorosi E.;
RT "Arabidopsis anaphase-promoting complexes: multiple activators and wide
RT range of substrates might keep APC perpetually busy.";
RL Cell Cycle 4:1084-1092(2005).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19074624; DOI=10.1104/pp.108.132449;
RA Larson-Rabin Z., Li Z., Masson P.H., Day C.D.;
RT "FZR2/CCS52A1 expression is a determinant of endoreduplication and cell
RT expansion in Arabidopsis.";
RL Plant Physiol. 149:874-884(2009).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19553203; DOI=10.1073/pnas.0901193106;
RA Vanstraelen M., Baloban M., Da Ines O., Cultrone A., Lammens T.,
RA Boudolf V., Brown S.C., De Veylder L., Mergaert P., Kondorosi E.;
RT "APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis
RT root.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:11806-11811(2009).
RN [8]
RP REVIEW, AND GENE FAMILY.
RX PubMed=21087491; DOI=10.1186/1471-2229-10-254;
RA Lima M.D.F., Eloy N.B., Pegoraro C., Sagit R., Rojas C., Bretz T.,
RA Vargas L., Elofsson A., de Oliveira A.C., Hemerly A.S., Ferreira P.C.G.;
RT "Genomic evolution and complexity of the Anaphase-promoting Complex (APC)
RT in land plants.";
RL BMC Plant Biol. 10:254-254(2010).
RN [9]
RP INTERACTION WITH GIG1 AND PYM.
RX PubMed=22167058; DOI=10.1105/tpc.111.092049;
RA Iwata E., Ikeda S., Matsunaga S., Kurata M., Yoshioka Y., Criqui M.-C.,
RA Genschik P., Ito M.;
RT "GIGAS CELL1, a novel negative regulator of the anaphase-promoting
RT complex/cyclosome, is required for proper mitotic progression and cell fate
RT determination in Arabidopsis.";
RL Plant Cell 23:4382-4393(2011).
RN [10]
RP INTERACTION WITH PYM.
RX PubMed=22167059; DOI=10.1105/tpc.111.091793;
RA Heyman J., Van den Daele H., De Wit K., Boudolf V., Berckmans B.,
RA Verkest A., Alvim Kamei C.L., De Jaeger G., Koncz C., De Veylder L.;
RT "Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by
RT temporal inhibition of the anaphase-promoting complex/cyclosome.";
RL Plant Cell 23:4394-4410(2011).
RN [11]
RP INTERACTION WITH CDC20-1 AND CDC20-2.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
RN [12]
RP INTERACTION WITH GIG1.
RX PubMed=22844260; DOI=10.1371/journal.pgen.1002865;
RA Cromer L., Heyman J., Touati S., Harashima H., Araou E., Girard C.,
RA Horlow C., Wassmann K., Schnittger A., De Veylder L., Mercier R.;
RT "OSD1 promotes meiotic progression via APC/C inhibition and forms a
RT regulatory network with TDM and CYCA1;2/TAM.";
RL PLoS Genet. 8:E1002865-E1002865(2012).
CC -!- FUNCTION: Activator protein that regulates the ubiquitin ligase
CC activity and substrate specificity of the anaphase promoting
CC complex/cyclosome (APC/C). Necessary and sufficient for
CC endoreduplication and correct cell expansion. Controls meristem size by
CC stimulating endoreduplication in the elongation zone.
CC {ECO:0000269|PubMed:15970679, ECO:0000269|PubMed:19074624,
CC ECO:0000269|PubMed:19553203}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Associates with the APC/C complex. Interacts with CDC20-1,
CC CDC20-2, CYCA1-1, CYCA1-2, CYCA3-4, CYCB1-1 AND CYCB1-2. Binds to GIG1
CC and PYM. {ECO:0000269|PubMed:15970679, ECO:0000269|PubMed:21687678,
CC ECO:0000269|PubMed:22167058, ECO:0000269|PubMed:22167059,
CC ECO:0000269|PubMed:22844260}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19553203}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, flowers, leaves and roots.
CC Expressed in the differentiating cell files of the root elongation
CC zone. {ECO:0000269|PubMed:19074624, ECO:0000269|PubMed:19553203}.
CC -!- DEVELOPMENTAL STAGE: Expressed from late M until late S-early G2
CC phases. {ECO:0000269|PubMed:15970679}.
CC -!- DISRUPTION PHENOTYPE: Reduced endoreduplication and reduced expansion
CC in trichomes and other leaf cells. Longer roots. Root meristem contains
CC more dividing cells with a delayed onset of endoreduplication.
CC {ECO:0000269|PubMed:19074624, ECO:0000269|PubMed:19553203}.
CC -!- MISCELLANEOUS: FZR2 controls the induction of early rounds of
CC endoreduplication while the remaining rounds may be mediated by FZR1
CC and FZR3.
CC -!- MISCELLANEOUS: FZR1 and FZR2 are functional homologs, and their
CC functional divergence in root development arises from the different
CC expression patterns. {ECO:0000305|PubMed:19553203}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19806.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79246.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Arabidopsis APC/C subunits;
CC URL="http://personal.rhul.ac.uk/ujba/110/apc/APC.htm";
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DR EMBL; AL031018; CAA19806.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161558; CAB79246.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84680.1; -; Genomic_DNA.
DR EMBL; AY099585; AAM20437.1; -; mRNA.
DR EMBL; AY128834; AAM91234.1; -; mRNA.
DR PIR; T05122; T05122.
DR RefSeq; NP_194022.3; NM_118420.5.
DR AlphaFoldDB; Q8L3Z8; -.
DR SMR; Q8L3Z8; -.
DR BioGRID; 13679; 29.
DR ELM; Q8L3Z8; -.
DR IntAct; Q8L3Z8; 21.
DR STRING; 3702.AT4G22910.1; -.
DR iPTMnet; Q8L3Z8; -.
DR PaxDb; Q8L3Z8; -.
DR PRIDE; Q8L3Z8; -.
DR ProteomicsDB; 230440; -.
DR EnsemblPlants; AT4G22910.1; AT4G22910.1; AT4G22910.
DR GeneID; 828390; -.
DR Gramene; AT4G22910.1; AT4G22910.1; AT4G22910.
DR KEGG; ath:AT4G22910; -.
DR Araport; AT4G22910; -.
DR TAIR; locus:2127263; AT4G22910.
DR eggNOG; KOG0305; Eukaryota.
DR HOGENOM; CLU_014831_4_1_1; -.
DR InParanoid; Q8L3Z8; -.
DR OMA; TEPRYKR; -.
DR OrthoDB; 1220675at2759; -.
DR PhylomeDB; Q8L3Z8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8L3Z8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8L3Z8; baseline and differential.
DR Genevisible; Q8L3Z8; AT.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:TAIR.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:TAIR.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0010091; P:trichome branching; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19918; PTHR19918; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome; Repeat;
KW Ubl conjugation pathway; WD repeat.
FT CHAIN 1..483
FT /note="Protein FIZZY-RELATED 2"
FT /id="PRO_0000364436"
FT REPEAT 174..211
FT /note="WD 1"
FT REPEAT 215..254
FT /note="WD 2"
FT REPEAT 257..294
FT /note="WD 3"
FT REPEAT 298..337
FT /note="WD 4"
FT REPEAT 340..382
FT /note="WD 5"
FT REPEAT 384..425
FT /note="WD 6"
FT REPEAT 428..467
FT /note="WD 7"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 483 AA; 52840 MW; CF4E5411B9DE1687 CRC64;
MEEEDPTASN VITNSNSSSM RNLSPAMNTP VVSLESRINR LINANQSQSP SPSSLSRSIY
SDRFIPSRSG SNFALFDLSP SPSKDGKEDG AGSYATLLRA AMFGPETPEK RDITGFSSSR
NIFRFKTETH RSLNSFSPFG VDDDSPGVSH SGPVKAPRKV PRSPYKVLDA PALQDDFYLN
LVDWSAQNVL AVGLGNCVYL WNACSSKVTK LCDLGAEDSV CSVGWALRGT HLAVGTSTGK
VQIWDASRCK RTRTMEGHRL RVGALAWGSS VLSSGSRDKS ILQRDIRCQE DHVSKLAGHK
SEVCGLKWSY DNRELASGGN DNRLFVWNQH STQPVLKYSE HTAAVKAIAW SPHVHGLLAS
GGGTADRCIR FWNTTTNTHL SSIDTCSQVC NLAWSKNVNE LVSTHGYSQN QIIVWKYPTM
SKIATLTGHT YRVLYLAVSP DGQTIVTGAG DETLRFWNVF PSPKSQNTDS EIGSSFFGRT
TIR
