G1094_PHOV8
ID G1094_PHOV8 Reviewed; 516 AA.
AC A6KWM1;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Glycosyl hydrolase family 109 protein 4;
DE EC=3.2.1.-;
DE Flags: Precursor;
GN OrderedLocusNames=BVU_0105;
OS Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 /
OS NBRC 14291 / NCTC 11154) (Bacteroides vulgatus).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Phocaeicola.
OX NCBI_TaxID=435590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC
RC 11154;
RX PubMed=17579514; DOI=10.1371/journal.pbio.0050156;
RA Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C.,
RA Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H.,
RA Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K.,
RA Knight R.D., Gordon J.I.;
RT "Evolution of symbiotic bacteria in the distal human intestine.";
RL PLoS Biol. 5:1574-1586(2007).
CC -!- FUNCTION: Glycosidase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC Note=Binds 1 NAD(+) per subunit. The NAD(+) cannot dissociate.
CC {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- SIMILARITY: Belongs to the Gfo/Idh/MocA family. Glycosyl hydrolase 109
CC subfamily. {ECO:0000305}.
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DR EMBL; CP000139; ABR37835.1; -; Genomic_DNA.
DR RefSeq; WP_005843044.1; NC_009614.1.
DR AlphaFoldDB; A6KWM1; -.
DR SMR; A6KWM1; -.
DR STRING; 435590.BVU_0105; -.
DR CAZy; GH109; Glycoside Hydrolase Family 109.
DR EnsemblBacteria; ABR37835; ABR37835; BVU_0105.
DR KEGG; bvu:BVU_0105; -.
DR eggNOG; COG0673; Bacteria.
DR HOGENOM; CLU_046965_0_0_10; -.
DR OMA; HPLWKKY; -.
DR OrthoDB; 1465613at2; -.
DR BioCyc; BVUL435590:G1G59-111-MON; -.
DR Proteomes; UP000002861; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR000683; Gfo/Idh/MocA-like_OxRdtase_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01408; GFO_IDH_MocA; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycosidase; Hydrolase; Lipoprotein; Membrane; NAD;
KW Palmitate; Reference proteome; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 19..516
FT /note="Glycosyl hydrolase family 109 protein 4"
FT /id="PRO_0000348556"
FT BINDING 76..77
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 98
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 146..149
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 166..167
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 195
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 224
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 247
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 259..262
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 259
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 337
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT LIPID 19
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 19
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 516 AA; 57404 MW; 21CE4700350390EE CRC64;
MKKIKLLLVA GACVVLSACS PQAPTVHTTD KGTQWEWNEG TIVVKSPERP AGQKSVIGLT
APKMEVVRVG FVGLGMRGPG AVSRFTHIPG TQIVALCDYD PKRAEACQNI LKKASMPKAA
IYSGETGYEE LCKRDDIDLV YIAADWLHHF PIAKCALENG KHVAIEVPSA MNLQECWDLV
NLSETTRKHC FILENCCYDW FEMNTLNMAQ NGVFGEVIRA QGAYIHNLSD FWDYYWKNGE
NDKLGWRLEF NMRHRGDVYA THGLGPVAQV LDIHRGDRMK TLTAMDTKSV VGKGLVEAKT
GSECTNFRNG DHTTTMIRTE NGKVIEIQHN VMTPQPYNRL YQLTGVKGFA NKYPTEGYAL
GADQLSASGV QPKVDNLSSH GFLPQAEMDA LVEKYQHPIL KKYGEIAKEV GGHGGMDFIM
DSRLVYCLQN GLPLDMDVYD LAEWCALAEL GEISMDNNCA AVEFPDFTRG EWNVVKGYKH
AYASPEEEQA SMEKAKAFTA KLKERGAKEW ATEADK
