ID   G1P_BPPHL               Reviewed;         440 AA.
AC   O55247;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Gene 1 protein;
DE   AltName: Full=G1P;
GN   Name=I;
OS   Xanthomonas phage phiLf (Bacteriophage phi-Lf).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; unclassified Inoviridae.
OX   NCBI_TaxID=28365;
OH   NCBI_TaxID=340; Xanthomonas campestris pv. campestris.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8654978; DOI=10.1016/0378-1119(96)00197-7;
RA   Wen F.S., Tseng Y.H.;
RT   "Nucleotide sequence of the gene presumably encoding the adsorption protein
RT   of filamentous phage phi Lf.";
RL   Gene 172:161-162(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9367841; DOI=10.1006/bbrc.1997.7548;
RA   Liu T.J., Wen F.S., Tseng T.T., Yang M.T., Lin N.T., Tseng Y.H.;
RT   "Identification of gene VI of filamentous phage phi Lf coding for a 10-kDa
RT   minor coat protein.";
RL   Biochem. Biophys. Res. Commun. 239:752-755(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9571147; DOI=10.1006/bbrc.1998.8432;
RA   Chang K.H., Wen F.S., Tseng T.T., Lin N.T., Yang M.T., Tseng Y.H.;
RT   "Sequence analysis and expression of the filamentous phage phi Lf gene I
RT   encoding a 48-kDa protein associated with host cell membrane.";
RL   Biochem. Biophys. Res. Commun. 245:313-318(1998).
CC   -!- FUNCTION: Isoform G1P plays an essential role in phage assembly. It is
CC       required to increase the number of adhesion zones between the inner and
CC       outer membranes of the host cell. The extrusion of neo-synthesized
CC       phages occurs at these adhesion sites. May be involved with G4P in
CC       creating zone through which the phage assembled and extruded (By
CC       similarity). {ECO:0000250}.
CC   -!- FUNCTION: Isoform G11P is also involved in phage assembly, probably
CC       playing a structural role in the formation of the phage assembly site.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with G4P; this interaction results in a complex that
CC       spans the inner an outer host membranes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=G1P; Synonyms=Gene 1 protein;
CC         IsoId=O55247-1; Sequence=Displayed;
CC       Name=G11P; Synonyms=Gene 11 protein;
CC         IsoId=O55247-2; Sequence=VSP_037576;
CC   -!- SIMILARITY: Belongs to the inovirus G1P protein family. {ECO:0000305}.
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DR   EMBL; AF018286; AAB88261.1; -; Genomic_DNA.
DR   PIR; JE0085; JE0085.
DR   Proteomes; UP000007611; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0099045; P:viral extrusion; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008900; Zot_N.
DR   Pfam; PF05707; Zot; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; ATP-binding; Host membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW   Viral extrusion; Viral release from host cell.
FT   CHAIN           1..440
FT                   /note="Gene 1 protein"
FT                   /id="PRO_0000378353"
FT   TRANSMEM        213..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          386..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          413..440
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           95..98
FT                   /note="DEAH box"
FT   COMPBIAS        421..440
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         7..14
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..249
FT                   /note="Missing (in isoform G11P)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_037576"
SQ   SEQUENCE   440 AA;  48137 MW;  2EF946E538CCD2E0 CRC64;
     MLVFNEGVPR AGKSYDAVKN HILPALKKGR RVFARLNGLR FDRIAKHLGM AENDVQHLLV
     LVDTKDVSKL FACTQDESGK WCIPDEFKDA LVVIDEVHEF YVNERKPLAP AVENFWALLG
     QNGGDAVIMT QWINRLHSAV KARIEKKNTF QKLTAIGMKG RYRVTYFHTT SPGKFEKVGG
     QTLKYDPAIF PLYDGYAPGA ENTEVYEEGG KNVWAAMAVR AAIFLTLGGV GIYFFMHYFT
     KDRADPNKPM ASASQTTKPT HVGAGFANGA PSVPIQPPPP DPLADLTQEQ RYVAQLADKG
     RIRLSARARV GDQDRAWIQW IDASNNVVEE LDLSQLRALG YSVSVVTYGV RLSAGKHIMV
     ATAWPWTAPI REKDARLYNM APDGSGGAAG VATAGSDGGG ADRDQVRGGV IEYGPRTQGT
     FPDNKGYSSS TSTPATTLQM