G23A_DROME
ID G23A_DROME Reviewed; 374 AA.
AC P83292; Q4V3K4; Q9VQE7;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Gustatory receptor 23a;
GN Name=Gr23a; Synonyms=GR23A.1; ORFNames=CG15396;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-374 (ISOFORM D).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10710312; DOI=10.1126/science.287.5459.1830;
RA Clyne P.J., Warr C.G., Carlson J.R.;
RT "Candidate taste receptors in Drosophila.";
RL Science 287:1830-1834(2000).
RN [5]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
CC -!- FUNCTION: Probable gustatory receptor which mediates acceptance or
CC avoidance behavior, depending on its substrates. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=B {ECO:0000312|FlyBase:FBgn0041248};
CC IsoId=P83292-1; Sequence=Displayed;
CC Name=C {ECO:0000312|FlyBase:FBgn0041248};
CC IsoId=P83292-2, Q9VQE7-1;
CC Sequence=VSP_059041;
CC Name=D;
CC IsoId=P83292-3; Sequence=VSP_059040;
CC -!- TISSUE SPECIFICITY: Expressed in the adult labellar chemosensory
CC neurons and labral sense organ. Expressed in neurons of the dorsal
CC pharyngeal sense organ of larvae. {ECO:0000269|PubMed:10710312,
CC ECO:0000269|PubMed:22031876}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr2a subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF51227.3; -; Genomic_DNA.
DR EMBL; AE014134; AAO41162.1; -; Genomic_DNA.
DR EMBL; BT023352; AAY55768.1; -; mRNA.
DR RefSeq; NP_523459.3; NM_078735.6. [P83292-2]
DR RefSeq; NP_787965.1; NM_175951.2. [P83292-1]
DR AlphaFoldDB; P83292; -.
DR SMR; P83292; -.
DR STRING; 7227.FBpp0077386; -.
DR PaxDb; P83292; -.
DR EnsemblMetazoa; FBtr0077702; FBpp0077386; FBgn0041248. [P83292-1]
DR EnsemblMetazoa; FBtr0273435; FBpp0271943; FBgn0041248. [P83292-2]
DR GeneID; 33453; -.
DR KEGG; dme:Dmel_CG15396; -.
DR CTD; 33453; -.
DR FlyBase; FBgn0041248; Gr23a.
DR VEuPathDB; VectorBase:FBgn0041248; -.
DR OMA; FWYGDAG; -.
DR PhylomeDB; P83292; -.
DR BioGRID-ORCS; 33453; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33453; -.
DR PRO; PR:P83292; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0041248; Expressed in mouthpart.
DR Genevisible; P83292; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..374
FT /note="Gustatory receptor 23a"
FT /id="PRO_0000216500"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..36
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 37..57
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 73..93
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..112
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 113..133
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 227..247
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..263
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 264..284
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 344..364
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..374
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..329
FT /note="MFPPTRVQASSRVVLKIFHFILVAFSLRSRRLSRLVLWLQFLGWLTWFISMW
FT TQSVIYAQTIDCTLDCSLRHILTFFQTVSHAFIVVTSFLDGFRIKQDQLDEPIAFEDSD
FT PWLAFTVLAMLVPTLGVEYLVCSNAPEYAFRIRIYHLKTLPSFLALQVQIISFILEVMK
FT VNIRVRQTKLQLLILARELSCRWPQRKQKPQFSDQQAHRVKDLKRRYNDLHYLFVRING
FT YFGGSLLTIIIVHFAIFVSNSYWLFVDIRTRPWRIYAILLNLGFIFNVALQMAAACWHC
FT QQSYNLGRQIGCLISKLVKPQGSKLYNDLVSEFSLQTLHQR -> MKTLECLTRRFLEV
FT IFSVLALVPLPPISQLGWLFLSLAIRCCWIVYFIYLLDVAISFSWVAIENVGNAVGTML
FT FVGNSVLGFALLLESVLKQKTHSQLEDLRVQTELQLQRLGMFGRSRHAAYLLPLIGVQF
FT TCDLVRLATNFGETVSPVFCISLPLMWLLRYRYVQLVQHVMDLNQRSIHLRRSLLSMAS
FT GNDLWQPYGVQECLQLQTLRTTYERIFECYETFSDCYGWGMLGLHLLTSFQFVTNAYWM
FT IMGIYDGGNVRSLIFNGATGIDFGTPIATLFWHGDSGAENNQAGPVGRTDCVRGLRSLV
FT GLHCAGDAGSHFGSRVFGVLERARICLSYQDLSPENAAQFSGSAGADYILYPGGHEGEH
FT KG (in isoform D)"
FT /id="VSP_059040"
FT VAR_SEQ 1..294
FT /note="MFPPTRVQASSRVVLKIFHFILVAFSLRSRRLSRLVLWLQFLGWLTWFISMW
FT TQSVIYAQTIDCTLDCSLRHILTFFQTVSHAFIVVTSFLDGFRIKQDQLDEPIAFEDSD
FT PWLAFTVLAMLVPTLGVEYLVCSNAPEYAFRIRIYHLKTLPSFLALQVQIISFILEVMK
FT VNIRVRQTKLQLLILARELSCRWPQRKQKPQFSDQQAHRVKDLKRRYNDLHYLFVRING
FT YFGGSLLTIIIVHFAIFVSNSYWLFVDIRTRPWRIYAILLNLGFIFNVALQMAAACWHC
FT QQSYNL -> MKTLECLTRRFLEVIFSVLALVPLPPISQLGWLFLSLAIRCCWIVYFIY
FT LLDVAISFSWVAIENVGNAVGTMLFVGNSVLGFALLLESVLKQKTHSQLEDLRVQTELQ
FT LQRLGMFGRSRHAAYLLPLIGVQFTCDLVRLATNFGETVSPVFCISLPLMWLLRYRYVQ
FT LVQHVMDLNQRSIHLRRSLLSMASGNDLWQPYGVQECLQLQTLRTTYERIFECYETFSD
FT CYGWGMLGLHLLTSFQFVTNAYWMIMGIYDGGNVRSLIFNGATGIDFGTPIATLFWHGD
FT SGAEN (in isoform C)"
FT /id="VSP_059041"
SQ SEQUENCE 374 AA; 43587 MW; 11FEE5851BD3319D CRC64;
MFPPTRVQAS SRVVLKIFHF ILVAFSLRSR RLSRLVLWLQ FLGWLTWFIS MWTQSVIYAQ
TIDCTLDCSL RHILTFFQTV SHAFIVVTSF LDGFRIKQDQ LDEPIAFEDS DPWLAFTVLA
MLVPTLGVEY LVCSNAPEYA FRIRIYHLKT LPSFLALQVQ IISFILEVMK VNIRVRQTKL
QLLILARELS CRWPQRKQKP QFSDQQAHRV KDLKRRYNDL HYLFVRINGY FGGSLLTIII
VHFAIFVSNS YWLFVDIRTR PWRIYAILLN LGFIFNVALQ MAAACWHCQQ SYNLGRQIGC
LISKLVKPQG SKLYNDLVSE FSLQTLHQRF VVTAKDFFSL NLHLLSSMFA AVVTYLVILI
QFMFAERSST RGSG
