G2L2L_XENLA
ID G2L2L_XENLA Reviewed; 980 AA.
AC A0A1L8HFX9;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=GAS2-like protein 2A;
DE AltName: Full=growth arrest specific 2 like 2 A;
GN Name=gas2l2.L {ECO:0000312|Xenbase:XB-GENE-17330203};
GN ORFNames=XELAEV_18012681mg {ECO:0000312|EMBL:OCT94996.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000312|Proteomes:UP000186698}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J {ECO:0000312|Proteomes:UP000186698};
RX PubMed=27762356; DOI=10.1038/nature19840;
RA Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL Nature 538:336-343(2016).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=30665704; DOI=10.1016/j.ajhg.2018.12.009;
RA Bustamante-Marin X.M., Yin W.N., Sears P.R., Werner M.E., Brotslaw E.J.,
RA Mitchell B.J., Jania C.M., Zeman K.L., Rogers T.D., Herring L.E.,
RA Refabert L., Thomas L., Amselem S., Escudier E., Legendre M., Grubb B.R.,
RA Knowles M.R., Zariwala M.A., Ostrowski L.E.;
RT "Lack of GAS2L2 Causes PCD by Impairing Cilia Orientation and Mucociliary
RT Clearance.";
RL Am. J. Hum. Genet. 104:229-245(2019).
CC -!- FUNCTION: Together with gas2l2.S, regulates ciliary orientation and
CC performance. {ECO:0000269|PubMed:30665704}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:30665704}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000269|PubMed:30665704}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of both gas2l2.L and
CC gas2l2.s resulted in mosaic embryos and impacted the number and
CC distribution of basal bodies on the surface of ciliated cells
CC (PubMed:30665704). As a result, ciliary orientation was significantly
CC reduced (PubMed:30665704). {ECO:0000269|PubMed:30665704}.
CC -!- SIMILARITY: Belongs to the GAS2 family. {ECO:0000305}.
CC -!- CAUTION: Due to the high similarity between gas2l2.L and gas2l2.S, the
CC morpholinos used for knockdown target both gas2l2.L and gas2l2.S RNA.
CC {ECO:0000269|PubMed:30665704}.
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DR EMBL; CM004468; OCT94996.1; -; Genomic_DNA.
DR RefSeq; XP_018102346.1; XM_018246857.1.
DR RefSeq; XP_018102347.1; XM_018246858.1.
DR AlphaFoldDB; A0A1L8HFX9; -.
DR SMR; A0A1L8HFX9; -.
DR STRING; 8355.A0A1L8HFX9; -.
DR GeneID; 108708297; -.
DR KEGG; xla:108708297; -.
DR CTD; 108708297; -.
DR Xenbase; XB-GENE-17330203; gas2l2.L.
DR OMA; QPCHFRN; -.
DR OrthoDB; 1343422at2759; -.
DR Proteomes; UP000186698; Chromosome 2L.
DR Bgee; 108708297; Expressed in neurula embryo and 3 other tissues.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; IMP:UniProtKB.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.30.920.20; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR003108; GAR_dom.
DR InterPro; IPR036534; GAR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF02187; GAS2; 1.
DR SMART; SM00033; CH; 1.
DR SMART; SM00243; GAS2; 1.
DR SUPFAM; SSF143575; SSF143575; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51460; GAR; 1.
PE 3: Inferred from homology;
KW Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..980
FT /note="GAS2-like protein 2A"
FT /id="PRO_0000448236"
FT DOMAIN 39..166
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 207..279
FT /note="GAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00792"
FT REGION 323..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 523..607
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 763..980
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..387
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..445
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 474..501
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 523..573
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 777..795
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 829..849
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 853..873
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 899..940
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 954..968
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 980 AA; 110778 MW; 3C93F6B0F44EFFCE CRC64;
MCIALSHSLA ALNSGIMSTH QGGSVRSIRP FKSSEEYLYA MKEDLAEWLK DMYHLEIIVD
NFTEILENGS VLCQHANNVT EVAREFSVQY PQLAEKLQLP KSGVKLNMMA QPGTFLARDN
VSNFIQWCRK EMDIKDVLMF ETEDLVLRKN EKNFVLCLLE LARRASRFGM SAPMLIQMEE
QIEEEIREEM DLPPPEIPVP KPQRKLCDFK NLDQMVQHLV SRCTCPVQFS MIKVSEGKYK
VGESSTLIFV RILRKHVMVR VGGGWDTLEH YLDKHDPCRC TSLSHKPAIK LGSLQKQVSA
VHEVKTRLTP RLDNPSKVET TLIVSRSQSP LPPVEWRSYS SGRTRTTSSS NSLDNNENSP
TFKMSPSDRR SMSTSYRAHE RSATPSRKLF TEDNPEPQQL GNPQSVNFRH HHSTSSLASQ
LTGSEEENSC LSEVFSESQS RRQMGRTTVM SLKEPAMKEA FLSHTQSAHR KVMSATEQNN
IQQGSRIVNQ SINSQDSRPK TPSRFIRPPS PSKQIHFFHN QQNKGVEQNP KTQINSCTRS
PSPVKQTSLT YKQEFTKRPA TPSRISSQYK DGDKSAATEI IPSQDHIKSK PSVSSEERTI
RTGRSTPTIH LNKNGITYRE VSNLPPRMQP KPDALELSHS IERDCMYTPL PIDPEQEKQI
YRSLEDEILT NIKVLEGDSE ENHPENDSHG FNMLEKSTLP LNASTLKNPS GQGSVPRSGV
YINTTWQSGA CYDDVITELT KGHVKLNRVD VENWISKIPS KGMVKEAEEA HTSQRNQVRE
NGHTGAVSQR TIVRKKTPST EIKGSKPKQL PSQRARQLAL ENKKKTVDNV SENQASTFSG
DSGETVQEKI KLSQGLKPKR TLKKPERVPS IYKLKLRPKI RPRRDNRPEK KPSRIPTPVS
YRQMPSRNNG KAVKRTQSYQ ANKTSAPQTL NESQSSTVIL EDLDSDDKTL EHSAPSQAVE
TPVTIGNDQS KTEEEEESWV
