G2L2S_XENLA
ID G2L2S_XENLA Reviewed; 956 AA.
AC A0A1L8H8C0;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=GAS2-like protein 2B;
DE AltName: Full=growth arrest specific 2 like 2 B;
GN Name=gas2l2.S {ECO:0000312|Xenbase:XB-GENE-17330204};
GN ORFNames=XELAEV_18015306mg {ECO:0000312|EMBL:OCT92251.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000312|Proteomes:UP000186698}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J {ECO:0000312|Proteomes:UP000186698};
RX PubMed=27762356; DOI=10.1038/nature19840;
RA Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL Nature 538:336-343(2016).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=30665704; DOI=10.1016/j.ajhg.2018.12.009;
RA Bustamante-Marin X.M., Yin W.N., Sears P.R., Werner M.E., Brotslaw E.J.,
RA Mitchell B.J., Jania C.M., Zeman K.L., Rogers T.D., Herring L.E.,
RA Refabert L., Thomas L., Amselem S., Escudier E., Legendre M., Grubb B.R.,
RA Knowles M.R., Zariwala M.A., Ostrowski L.E.;
RT "Lack of GAS2L2 Causes PCD by Impairing Cilia Orientation and Mucociliary
RT Clearance.";
RL Am. J. Hum. Genet. 104:229-245(2019).
CC -!- FUNCTION: Together with gas2l2.L, regulates ciliary orientation and
CC performance. {ECO:0000269|PubMed:30665704}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}. Cytoplasm,
CC cytoskeleton, cilium basal body {ECO:0000269|PubMed:30665704}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of both gas2l2.L and
CC gas2l2.s resulted in mosiac embryos and impacted the number and
CC distribution of basal bodies on the surface of ciliated cells
CC (PubMed:30665704). As a result, ciliary orientation was significantly
CC reduced (PubMed:30665704). {ECO:0000269|PubMed:30665704}.
CC -!- SIMILARITY: Belongs to the GAS2 family. {ECO:0000305}.
CC -!- CAUTION: Due to the high similarity between gas2l2.L and gas2l2.S, the
CC morpholinos used for knockdown target both gas2l2.L and gas2l2.S RNA.
CC {ECO:0000269|PubMed:30665704}.
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DR EMBL; CM004469; OCT92251.1; -; Genomic_DNA.
DR RefSeq; XP_018105119.1; XM_018249630.1.
DR AlphaFoldDB; A0A1L8H8C0; -.
DR SMR; A0A1L8H8C0; -.
DR STRING; 8355.A0A1L8H8C0; -.
DR GeneID; 108709619; -.
DR KEGG; xla:108709619; -.
DR CTD; 108709619; -.
DR Xenbase; XB-GENE-17330204; gas2l2.S.
DR OrthoDB; 1343422at2759; -.
DR Proteomes; UP000186698; Chromosome 2S.
DR Bgee; 108709619; Expressed in neurula embryo and 1 other tissue.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; IMP:UniProtKB.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.30.920.20; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR003108; GAR_dom.
DR InterPro; IPR036534; GAR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF02187; GAS2; 1.
DR SMART; SM00033; CH; 1.
DR SMART; SM00243; GAS2; 1.
DR SUPFAM; SSF143575; SSF143575; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51460; GAR; 1.
PE 3: Inferred from homology;
KW Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..956
FT /note="GAS2-like protein 2B"
FT /id="PRO_0000448237"
FT DOMAIN 23..150
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 191..263
FT /note="GAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00792"
FT REGION 332..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 853..885
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 914..956
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..357
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 956 AA; 108436 MW; D84D1DA436B59816 CRC64;
MSTHQGGSVH SIRPFKSSEE YLYAMKEDLA EWLKDMYHLE IIVDNFIEIL ENGSVLCQHA
NNVTEVAREF FVQYPHLAEK LQLPKSGVKL NMMAQPGSFL ARDNVSNFIQ WCRKEMDIKD
VIMFETEDLV HRKNEKNFLL CLLELARRAS RFGMSAPMLI QMEEQIEEEI RQEMDLPPQE
IPVPKPQRKL CDFKNLDQMV QNLVSRCTCP VQFSMVKVSE GKYRVGESST LIFVRILRKH
VMVRVGGGWD TLAHYLDKHD PCHCASLSHK PAIKLGSLQK QVSAVHEVKT QLTPRLDNPS
KLETTLILSR SQSPLPPVEW RTYSAERTRT ISSSYSLDDN ENSPSFKNSQ TPPNDRRSMS
IGAHERSATL SRKLFTEDTQ DPQQLGNPQS GHYRHHHSTS SLASQLTGSE EEYSYISEVF
SESQSGRQMG KHTVMNLKEP PKRDAFLSHT QSANRKVISA TQQNNIQQGL IIGHQSVTVQ
DSRPKTPSRF IQPPSPSKQI HFFYNQQNKG VEQNIKTQTN SCTRSSSPVK QTSLTYKQEF
NKRPATPSRI NYSSSQYKDG DKSAATEIIT FHDHVRAKPS VSSEERTIRT GRATPTIHLN
RENTNLPLKM QPKPNVTESS HSIERDCMYT PLPIDPEQEK QIYRSLEDEI RTNIKILEGD
SEENNPENDT AAHDFNVLGK STLRLSSSTS KTPRGQEGVP RSGVYINTAW QSGASYDDVI
TELTKGHVKL NNVDVENWIS KIPLKGMVKE AHTSQRNKVR ENGHTASLVR KKTLSTEMKG
SKQKQLPSQI ARQLALENKK KTVENVLEKQ VSTVSGDSKE AIPEKIKLSQ GLKPKKSLKK
PERVPSIYKL KLRPKIRPRR DNRPEKKPSR IPTPVSYRQV PSRNNAKALK RTQSYQANKV
SVAQTLNESQ ISAVNSEDLD SGDEICEHSA PLQAVETPIT GENNQSRTEE EEESWV
