G2OX3_ARATH
ID G2OX3_ARATH Reviewed; 335 AA.
AC O64692;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Gibberellin 2-beta-dioxygenase 3;
DE EC=1.14.11.13;
DE AltName: Full=GA 2-oxidase 3;
DE AltName: Full=Gibberellin 2-beta-hydroxylase 3;
DE AltName: Full=Gibberellin 2-oxidase 3;
GN Name=GA2OX3; OrderedLocusNames=At2g34555; ORFNames=T31E10.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10200325; DOI=10.1073/pnas.96.8.4698;
RA Thomas S.G., Phillips A.L., Hedden P.;
RT "Molecular cloning and functional expression of gibberellin 2-oxidases,
RT multifunctional enzymes involved in gibberellin deactivation.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:4698-4703(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16139211; DOI=10.1016/j.cub.2005.07.023;
RA Jasinski S., Piazza P., Craft J., Hay A., Woolley L., Rieu I., Phillips A.,
RA Hedden P., Tsiantis M.;
RT "KNOX action in Arabidopsis is mediated by coordinate regulation of
RT cytokinin and gibberellin activities.";
RL Curr. Biol. 15:1560-1565(2005).
RN [5]
RP INDUCTION BY AUXIN AND PACLOBUTRAZOL.
RX PubMed=16905669; DOI=10.1104/pp.106.084871;
RA Frigerio M., Alabadi D., Perez-Gomez J., Garcia-Carcel L., Phillips A.L.,
RA Hedden P., Blazquez M.A.;
RT "Transcriptional regulation of gibberellin metabolism genes by auxin
RT signaling in Arabidopsis.";
RL Plant Physiol. 142:553-563(2006).
RN [6]
RP FUNCTION.
RX PubMed=18805991; DOI=10.1105/tpc.108.058818;
RA Rieu I., Eriksson S., Powers S.J., Gong F., Griffiths J., Woolley L.,
RA Benlloch R., Nilsson O., Thomas S.G., Hedden P., Phillips A.L.;
RT "Genetic analysis reveals that C19-GA 2-oxidation is a major gibberellin
RT inactivation pathway in Arabidopsis.";
RL Plant Cell 20:2420-2436(2008).
RN [7]
RP GENE FAMILY.
RX PubMed=21056641; DOI=10.1016/j.gene.2010.10.010;
RA Han F., Zhu B.;
RT "Evolutionary analysis of three gibberellin oxidase genes in rice,
RT Arabidopsis, and soybean.";
RL Gene 473:23-35(2011).
CC -!- FUNCTION: Catalyzes the 2-beta-hydroxylation of several biologically
CC active gibberellins, leading to the homeostatic regulation of their
CC endogenous level. Catabolism of gibberellins (GAs) plays a central role
CC in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to
CC GA34/GA8. {ECO:0000269|PubMed:10200325, ECO:0000269|PubMed:18805991}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A1 + O2 = CO2 + gibberellin A8 +
CC succinate; Xref=Rhea:RHEA:15005, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58524, ChEBI:CHEBI:58594; EC=1.14.11.13;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC -!- TISSUE SPECIFICITY: Not expressed in the apex.
CC {ECO:0000269|PubMed:16139211}.
CC -!- INDUCTION: Up-regulated by auxin and paclobutrazol.
CC {ECO:0000269|PubMed:16905669}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA2OX subfamily. {ECO:0000305}.
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DR EMBL; AJ132437; CAB41009.1; -; mRNA.
DR EMBL; AC004077; AAM14908.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08989.1; -; Genomic_DNA.
DR PIR; T52577; T52577.
DR RefSeq; NP_181002.1; NM_129007.2.
DR AlphaFoldDB; O64692; -.
DR SMR; O64692; -.
DR STRING; 3702.AT2G34555.1; -.
DR PaxDb; O64692; -.
DR PRIDE; O64692; -.
DR ProteomicsDB; 248475; -.
DR EnsemblPlants; AT2G34555.1; AT2G34555.1; AT2G34555.
DR GeneID; 818019; -.
DR Gramene; AT2G34555.1; AT2G34555.1; AT2G34555.
DR KEGG; ath:AT2G34555; -.
DR Araport; AT2G34555; -.
DR TAIR; locus:505006289; AT2G34555.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_16_3_1; -.
DR InParanoid; O64692; -.
DR OMA; NLCLESH; -.
DR OrthoDB; 622449at2759; -.
DR PhylomeDB; O64692; -.
DR BioCyc; ARA:AT2G34555-MON; -.
DR BioCyc; MetaCyc:AT2G34555-MON; -.
DR BRENDA; 1.14.11.13; 399.
DR UniPathway; UPA00390; -.
DR PRO; PR:O64692; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64692; baseline and differential.
DR Genevisible; O64692; AT.
DR GO; GO:0052634; F:C-19 gibberellin 2-beta-dioxygenase activity; IBA:GO_Central.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IDA:CACAO.
DR GO; GO:0045487; P:gibberellin catabolic process; IBA:GO_Central.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..335
FT /note="Gibberellin 2-beta-dioxygenase 3"
FT /id="PRO_0000067307"
FT DOMAIN 175..278
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT ACT_SITE 269
FT /evidence="ECO:0000255"
FT BINDING 202
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 204
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 259
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 335 AA; 38216 MW; 181F6EAA1EE1C331 CRC64;
MVIVLQPASF DSNLYVNPKC KPRPVLIPVI DLTDSDAKTQ IVKACEEFGF FKVINHGVRP
DLLTQLEQEA INFFALHHSL KDKAGPPDPF GYGTKRIGPN GDLGWLEYIL LNANLCLESH
KTTAIFRHTP AIFREAVEEY IKEMKRMSSK FLEMVEEELK IEPKEKLSRL VKVKESDSCL
RMNHYPEKEE TPVKEEIGFG EHTDPQLISL LRSNDTEGLQ ICVKDGTWVD VTPDHSSFFV
LVGDTLQVMT NGRFKSVKHR VVTNTKRSRI SMIYFAGPPL SEKIAPLSCL VPKQDDCLYN
EFTWSQYKLS AYKTKLGDYR LGLFEKRPPF SLSNV
