G2OX4_ARATH
ID G2OX4_ARATH Reviewed; 321 AA.
AC Q9C7Z1;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Gibberellin 2-beta-dioxygenase 4;
DE EC=1.14.11.13;
DE AltName: Full=GA 2-oxidase 4;
DE AltName: Full=Gibberellin 2-beta-hydroxylase 4;
DE AltName: Full=Gibberellin 2-oxidase 4;
GN Name=GA2OX4; OrderedLocusNames=At1g47990; ORFNames=T2J15.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RA Rieu I., Thomas S.G., Phillips A.L., Hedden P.;
RT "Recombinant AtGA2ox4 shows gibberellin 2-oxidase activity in vitro.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16139211; DOI=10.1016/j.cub.2005.07.023;
RA Jasinski S., Piazza P., Craft J., Hay A., Woolley L., Rieu I., Phillips A.,
RA Hedden P., Tsiantis M.;
RT "KNOX action in Arabidopsis is mediated by coordinate regulation of
RT cytokinin and gibberellin activities.";
RL Curr. Biol. 15:1560-1565(2005).
RN [5]
RP INDUCTION BY AUXIN AND PACLOBUTRAZOL, AND TISSUE SPECIFICITY.
RX PubMed=16905669; DOI=10.1104/pp.106.084871;
RA Frigerio M., Alabadi D., Perez-Gomez J., Garcia-Carcel L., Phillips A.L.,
RA Hedden P., Blazquez M.A.;
RT "Transcriptional regulation of gibberellin metabolism genes by auxin
RT signaling in Arabidopsis.";
RL Plant Physiol. 142:553-563(2006).
RN [6]
RP FUNCTION.
RX PubMed=18805991; DOI=10.1105/tpc.108.058818;
RA Rieu I., Eriksson S., Powers S.J., Gong F., Griffiths J., Woolley L.,
RA Benlloch R., Nilsson O., Thomas S.G., Hedden P., Phillips A.L.;
RT "Genetic analysis reveals that C19-GA 2-oxidation is a major gibberellin
RT inactivation pathway in Arabidopsis.";
RL Plant Cell 20:2420-2436(2008).
RN [7]
RP GENE FAMILY.
RX PubMed=21056641; DOI=10.1016/j.gene.2010.10.010;
RA Han F., Zhu B.;
RT "Evolutionary analysis of three gibberellin oxidase genes in rice,
RT Arabidopsis, and soybean.";
RL Gene 473:23-35(2011).
CC -!- FUNCTION: Catalyzes the 2-beta-hydroxylation of several biologically
CC active gibberellins, leading to the homeostatic regulation of their
CC endogenous level. Catabolism of gibberellins (GAs) plays a central role
CC in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to
CC GA34/GA8. {ECO:0000269|PubMed:18805991, ECO:0000269|Ref.1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A1 + O2 = CO2 + gibberellin A8 +
CC succinate; Xref=Rhea:RHEA:15005, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58524, ChEBI:CHEBI:58594; EC=1.14.11.13;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC -!- TISSUE SPECIFICITY: Expressed at the base of the shoot apical meristem
CC and developing leaf primordia. {ECO:0000269|PubMed:16139211,
CC ECO:0000269|PubMed:16905669}.
CC -!- INDUCTION: Not regulated by auxin. Down-regulated by paclobutrazol.
CC {ECO:0000269|PubMed:16905669}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA2OX subfamily. {ECO:0000305}.
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DR EMBL; AY859740; AAW56769.1; -; mRNA.
DR EMBL; AC051631; AAG51528.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32235.1; -; Genomic_DNA.
DR PIR; C96520; C96520.
DR RefSeq; NP_175233.1; NM_103695.2.
DR AlphaFoldDB; Q9C7Z1; -.
DR SMR; Q9C7Z1; -.
DR STRING; 3702.AT1G47990.1; -.
DR PaxDb; Q9C7Z1; -.
DR PRIDE; Q9C7Z1; -.
DR EnsemblPlants; AT1G47990.1; AT1G47990.1; AT1G47990.
DR GeneID; 841217; -.
DR Gramene; AT1G47990.1; AT1G47990.1; AT1G47990.
DR KEGG; ath:AT1G47990; -.
DR Araport; AT1G47990; -.
DR TAIR; locus:2202587; AT1G47990.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_16_3_1; -.
DR InParanoid; Q9C7Z1; -.
DR OMA; ARMSMAY; -.
DR OrthoDB; 622449at2759; -.
DR PhylomeDB; Q9C7Z1; -.
DR BRENDA; 1.14.11.13; 399.
DR UniPathway; UPA00390; -.
DR PRO; PR:Q9C7Z1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7Z1; baseline and differential.
DR Genevisible; Q9C7Z1; AT.
DR GO; GO:0052634; F:C-19 gibberellin 2-beta-dioxygenase activity; IDA:TAIR.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0045487; P:gibberellin catabolic process; IDA:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..321
FT /note="Gibberellin 2-beta-dioxygenase 4"
FT /id="PRO_0000422355"
FT DOMAIN 156..269
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT ACT_SITE 260
FT /evidence="ECO:0000255"
FT BINDING 193
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 195
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 250
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 321 AA; 35873 MW; 2802A9CE2B30C973 CRC64;
MVKGSQKIVA VDQDIPIIDM SQERSQVSMQ IVKACESLGF FKVINHGVDQ TTISRMEQES
INFFAKPAHE KKSVRPVNQP FRYGFRDIGL NGDSGEVEYL LFHTNDPAFR SQLSFSSAVN
CYIEAVKQLA REILDLTAEG LHVPPHSFSR LISSVDSDSV LRVNHYPPSD QFFGEANLSD
QSVSLTRVGF GEHTDPQILT VLRSNGVGGL QVSNSDGMWV SVSPDPSAFC VNVGDLLQVM
TNGRFISVRH RALTYGEESR LSTAYFAGPP LQAKIGPLSA MVMTMNQPRL YQTFTWGEYK
KRAYSLRLED SRLDMFRTCK D
