G2OX6_ARATH
ID G2OX6_ARATH Reviewed; 329 AA.
AC Q9FZ21; Q56Y91;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Gibberellin 2-beta-dioxygenase 6;
DE EC=1.14.11.13;
DE AltName: Full=GA 2-oxidase 6;
DE AltName: Full=Gibberellin 2-beta-hydroxylase 6;
DE AltName: Full=Gibberellin 2-oxidase 6;
GN Name=GA2OX6; OrderedLocusNames=At1g02400; ORFNames=T6A9.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RA Rieu I., Thomas S.G., Phillips A.L., Hedden P.;
RT "Recombinant AtGA2ox6 shows gibberellin 2-oxidase activity in vitro.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidosis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY AUXIN AND PACLOBUTRAZOL.
RX PubMed=16905669; DOI=10.1104/pp.106.084871;
RA Frigerio M., Alabadi D., Perez-Gomez J., Garcia-Carcel L., Phillips A.L.,
RA Hedden P., Blazquez M.A.;
RT "Transcriptional regulation of gibberellin metabolism genes by auxin
RT signaling in Arabidopsis.";
RL Plant Physiol. 142:553-563(2006).
RN [7]
RP FUNCTION.
RX PubMed=18805991; DOI=10.1105/tpc.108.058818;
RA Rieu I., Eriksson S., Powers S.J., Gong F., Griffiths J., Woolley L.,
RA Benlloch R., Nilsson O., Thomas S.G., Hedden P., Phillips A.L.;
RT "Genetic analysis reveals that C19-GA 2-oxidation is a major gibberellin
RT inactivation pathway in Arabidopsis.";
RL Plant Cell 20:2420-2436(2008).
RN [8]
RP GENE FAMILY.
RX PubMed=21056641; DOI=10.1016/j.gene.2010.10.010;
RA Han F., Zhu B.;
RT "Evolutionary analysis of three gibberellin oxidase genes in rice,
RT Arabidopsis, and soybean.";
RL Gene 473:23-35(2011).
CC -!- FUNCTION: Catalyzes the 2-beta-hydroxylation of several biologically
CC active gibberellins, leading to the homeostatic regulation of their
CC endogenous level. Catabolism of gibberellins (GAs) plays a central role
CC in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to
CC GA34/GA8. Has no C20-GA 2 oxidase activity against GA12 and GA53.
CC {ECO:0000269|PubMed:18805991, ECO:0000269|Ref.1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A1 + O2 = CO2 + gibberellin A8 +
CC succinate; Xref=Rhea:RHEA:15005, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58524, ChEBI:CHEBI:58594; EC=1.14.11.13;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC -!- INDUCTION: Up-regulated by auxin. Down-regulated by paclobutrazol.
CC {ECO:0000269|PubMed:16905669}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA2OX subfamily. {ECO:0000305}.
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DR EMBL; AY859741; AAW56770.1; -; mRNA.
DR EMBL; AC064879; AAG00891.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27425.1; -; Genomic_DNA.
DR EMBL; BT014886; AAT42378.1; -; mRNA.
DR EMBL; AK228830; BAF00726.1; -; mRNA.
DR EMBL; AK221432; BAD94454.1; -; mRNA.
DR PIR; D86154; D86154.
DR RefSeq; NP_171742.1; NM_100121.5.
DR AlphaFoldDB; Q9FZ21; -.
DR SMR; Q9FZ21; -.
DR STRING; 3702.AT1G02400.1; -.
DR PaxDb; Q9FZ21; -.
DR PRIDE; Q9FZ21; -.
DR ProteomicsDB; 248599; -.
DR EnsemblPlants; AT1G02400.1; AT1G02400.1; AT1G02400.
DR GeneID; 839508; -.
DR Gramene; AT1G02400.1; AT1G02400.1; AT1G02400.
DR KEGG; ath:AT1G02400; -.
DR Araport; AT1G02400; -.
DR TAIR; locus:2204823; AT1G02400.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_16_3_1; -.
DR InParanoid; Q9FZ21; -.
DR OMA; KPRMSMM; -.
DR OrthoDB; 830141at2759; -.
DR PhylomeDB; Q9FZ21; -.
DR BRENDA; 1.14.11.13; 399.
DR UniPathway; UPA00390; -.
DR PRO; PR:Q9FZ21; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FZ21; baseline and differential.
DR Genevisible; Q9FZ21; AT.
DR GO; GO:0052634; F:C-19 gibberellin 2-beta-dioxygenase activity; IDA:TAIR.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0045487; P:gibberellin catabolic process; IDA:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR GO; GO:0009639; P:response to red or far red light; IEP:TAIR.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..329
FT /note="Gibberellin 2-beta-dioxygenase 6"
FT /id="PRO_0000422356"
FT DOMAIN 175..280
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT ACT_SITE 271
FT /evidence="ECO:0000255"
FT BINDING 203
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 205
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 260
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 329 AA; 36838 MW; 7D106DF0449C5A13 CRC64;
MVLPSSTPLQ TTGKKTISSP EYNFPVIDFS LNDRSKLSEK IVKACEVNGF FKVINHGVKP
EIIKRFEHEG EEFFNKPESD KLRAGPASPF GYGCKNIGFN GDLGELEYLL LHANPTAVAD
KSETISHDDP FKFSSATNDY IRTVRDLACE IIDLTIENLW GQKSSEVSEL IRDVRSDSIL
RLNHYPPAPY ALSGVGQIGF GEHSDPQILT VLRSNDVDGL EICSRDGLWI PIPSDPTCFF
VLVGDCLQAL TNGRFTSVRH RVLANTAKKP RMSAMYFAAP PLEAKISPLP KMVSPENPRR
YNSFTWGDYK KATYSLRLDV PRLEFFKTL
