G3O23_WHEAT
ID G3O23_WHEAT Reviewed; 369 AA.
AC Q3I409;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Gibberellin 3-beta-dioxygenase 2-3;
DE EC=1.14.11.15;
DE AltName: Full=Gibberellin 3 beta-hydroxylase 2-3;
DE AltName: Full=Gibberellin 3-oxidase 2-3;
GN Name=GA3ox2-3;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Maris Huntsman; TISSUE=Scutellum;
RX PubMed=16160850; DOI=10.1007/s00425-005-0104-0;
RA Appleford N.E., Evans D.J., Lenton J.R., Gaskin P., Croker S.J.,
RA Devos K.M., Phillips A.L., Hedden P.;
RT "Function and transcript analysis of gibberellin-biosynthetic enzymes in
RT wheat.";
RL Planta 223:568-582(2006).
CC -!- FUNCTION: Converts the inactive gibberellin precursors GA9 and GA20 in
CC the bioactives gibberellins GA4 and GA1. {ECO:0000269|PubMed:16160850}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A20 + O2 = CO2 + gibberellin A1 +
CC succinate; Xref=Rhea:RHEA:10104, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58524, ChEBI:CHEBI:58526; EC=1.14.11.15;
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA3OX subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ118252; AAZ94379.1; -; mRNA.
DR AlphaFoldDB; Q3I409; -.
DR SMR; Q3I409; -.
DR STRING; 4565.Traes_3B_791A6E8DF.1; -.
DR EnsemblPlants; TraesCAD_scaffold_041986_01G000200.1; TraesCAD_scaffold_041986_01G000200.1; TraesCAD_scaffold_041986_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_003708_01G000100.1; TraesCLE_scaffold_003708_01G000100.1; TraesCLE_scaffold_003708_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_005851_01G000100.1; TraesPAR_scaffold_005851_01G000100.1; TraesPAR_scaffold_005851_01G000100.
DR EnsemblPlants; TraesROB_scaffold_007358_01G000100.1; TraesROB_scaffold_007358_01G000100.1; TraesROB_scaffold_007358_01G000100.
DR Gramene; TraesCAD_scaffold_041986_01G000200.1; TraesCAD_scaffold_041986_01G000200.1; TraesCAD_scaffold_041986_01G000200.
DR Gramene; TraesCLE_scaffold_003708_01G000100.1; TraesCLE_scaffold_003708_01G000100.1; TraesCLE_scaffold_003708_01G000100.
DR Gramene; TraesPAR_scaffold_005851_01G000100.1; TraesPAR_scaffold_005851_01G000100.1; TraesPAR_scaffold_005851_01G000100.
DR Gramene; TraesROB_scaffold_007358_01G000100.1; TraesROB_scaffold_007358_01G000100.1; TraesROB_scaffold_007358_01G000100.
DR eggNOG; KOG0143; Eukaryota.
DR BioCyc; MetaCyc:MON-11646; -.
DR BRENDA; 1.14.11.15; 6500.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; Q3I409; baseline and differential.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0016707; F:gibberellin 3-beta-dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IBA:GO_Central.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..369
FT /note="Gibberellin 3-beta-dioxygenase 2-3"
FT /id="PRO_0000067319"
FT DOMAIN 205..306
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT ACT_SITE 297
FT /evidence="ECO:0000255"
FT BINDING 229
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 231
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 287
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 369 AA; 40326 MW; FC13E8D7908274F8 CRC64;
MPTPAHLSKD PHYFDFRAAR RVPETHAWPG LHDHPVVDGS GAGGEPDAVP VVDMRDPFAA
EAVGLAAQDW GAFLLVGHGV PLDLLVRVEA AIAGMFALPA SEKMRAVRRP GDSCGYGSPP
ISSFFSKCMW SEGYTFSPAN LRSDLRKLWP KAGHDYRHFC AVMEEFHREM RALADKLLEL
FLVALGLTGE QVAAVESEQK IAETMTATMH LNWYPKCPDP KRALGLIAHT DSGFFTFVLQ
SLVPGLQLFR HGPDRWVTVP AVPGAMVVNV GDLFQILTNG RFHSVYHRAV VNRESDRISL
GYFLGPPAHV KVAPLREALA GTPAAYRAVT WPEYMGVRKK AFTTGASALK MVAISTDDAA
NDTDDLILS
