G3PT_MESAU
ID G3PT_MESAU Reviewed; 100 AA.
AC P86211;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, testis-specific {ECO:0000250|UniProtKB:Q64467};
DE EC=1.2.1.12;
DE AltName: Full=Spermatogenic glyceraldehyde-3-phosphate dehydrogenase {ECO:0000250|UniProtKB:Q64467};
DE Flags: Fragments;
GN Name=GAPDHS {ECO:0000250|UniProtKB:Q64467};
OS Mesocricetus auratus (Golden hamster).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Mesocricetus.
OX NCBI_TaxID=10036;
RN [1]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=20400973; DOI=10.1038/aja.2010.19;
RA Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.;
RT "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen
RT (GP96) are unique to hamster caput epididymal spermatozoa.";
RL Asian J. Androl. 12:344-355(2010).
CC -!- FUNCTION: May play an important role in regulating the switch between
CC different pathways for energy production during spermiogenesis and in
CC the spermatozoon. Required for sperm motility and male fertility (By
CC similarity). {ECO:0000250|UniProtKB:Q64467}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-
CC phospho-glyceroyl phosphate + H(+) + NADH; Xref=Rhea:RHEA:10300,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57604, ChEBI:CHEBI:57945, ChEBI:CHEBI:59776; EC=1.2.1.12;
CC Evidence={ECO:0000250|UniProtKB:Q64467, ECO:0000255|PROSITE-
CC ProRule:PRU10009};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 1/5. {ECO:0000250|UniProtKB:Q64467}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q64467}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q64467}.
CC -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase
CC family. {ECO:0000255}.
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DR AlphaFoldDB; P86211; -.
DR UniPathway; UPA00109; UER00184.
DR Proteomes; UP000189706; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR10836; PTHR10836; 2.
DR SMART; SM00846; Gp_dh_N; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Glycolysis; NAD; Oxidoreductase; Reference proteome.
FT CHAIN <1..>100
FT /note="Glyceraldehyde-3-phosphate dehydrogenase, testis-
FT specific"
FT /id="PRO_0000394396"
FT BINDING 39
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O14556"
FT BINDING 64
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O14556"
FT BINDING 89
FT /ligand="D-glyceraldehyde 3-phosphate"
FT /ligand_id="ChEBI:CHEBI:59776"
FT /evidence="ECO:0000250|UniProtKB:Q64467"
FT NON_CONS 22..23
FT /evidence="ECO:0000305"
FT NON_CONS 33..34
FT /evidence="ECO:0000305"
FT NON_CONS 59..60
FT /evidence="ECO:0000305"
FT NON_CONS 80..81
FT /evidence="ECO:0000305"
FT NON_CONS 89..90
FT /evidence="ECO:0000305"
FT NON_TER 1
FT NON_TER 100
SQ SEQUENCE 100 AA; 11166 MW; 51163C88EE924823 CRC64;
DRCPCPCPCP CPCPVIRPPP PKELTVGING FGRVVAVNDP FIDPEYMVYM FKYDSTHGRR
VVVTAPSPDA PMFVMGVNEK GKLTGMAFRV VDLLRYMFSR
