ID   G3P_BPPF1               Reviewed;         437 AA.
AC   P25129;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   29-SEP-2021, entry version 84.
DE   RecName: Full=Attachment protein G3P;
DE   AltName: Full=Gene 3 protein;
DE            Short=G3P;
DE   AltName: Full=Minor coat protein;
DE   Flags: Precursor;
GN   Name=III;
OS   Pseudomonas phage Pf1 (Bacteriophage Pf1).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Primolicivirus.
OX   NCBI_TaxID=2011081;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25102-B1 / pf;
RX   PubMed=2010913; DOI=10.1016/0022-2836(91)90717-k;
RA   Hill D.F., Short N.J., Perham R.N., Petersen G.B.;
RT   "DNA sequence of the filamentous bacteriophage Pf1.";
RL   J. Mol. Biol. 218:349-364(1991).
RN   [2]
RP   FUNCTION.
RX   PubMed=16298408; DOI=10.1016/j.virol.2005.10.020;
RA   Holland S.J., Sanz C., Perham R.N.;
RT   "Identification and specificity of pilus adsorption proteins of filamentous
RT   bacteriophages infecting Pseudomonas aeruginosa.";
RL   Virology 345:540-548(2006).
CC   -!- FUNCTION: Plays essential roles both in the penetration of the viral
CC       genome into the bacterial host via pilus retraction and in the
CC       extrusion process. During the initial step of infection, G3P mediates
CC       adsorption of the phage to its primary receptor, the tip of host type
CC       IV PAK pilus. Subsequent interaction with the host entry receptor
CC       induces penetration of the viral DNA into the host cytoplasm. In the
CC       extrusion process, G3P mediates the release of the membrane-anchored
CC       virion from the cell via its C-terminal domain.
CC       {ECO:0000269|PubMed:16298408}.
CC   -!- SUBUNIT: Interacts with G6P; this interaction is required for proper
CC       integration of G3P and G6P into the virion. Interacts with G8P (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane
CC       {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
CC       Note=Prior to assembly, G3P is found associated with the bacterial host
CC       inner membrane. There are about five copies of this protein per mature
CC       phage that are located on the head side of the filamentous virion.
CC   -!- SIMILARITY: Belongs to the inovirus G3P protein family. {ECO:0000305}.
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DR   EMBL; X52107; CAA36332.1; -; Genomic_DNA.
DR   PIR; S15144; S15144.
DR   Proteomes; UP000002121; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0039667; P:viral entry into host cell via pilus retraction; IEA:UniProtKB-KW.
DR   GO; GO:0099045; P:viral extrusion; IEA:UniProtKB-KW.
DR   GO; GO:0039666; P:virion attachment to host cell pilus; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Host membrane; Host-virus interaction; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix;
KW   Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW   Viral attachment to host cell pilus;
KW   Viral attachment to host entry receptor; Viral extrusion;
KW   Viral penetration into host cytoplasm;
KW   Viral penetration into host cytoplasm via pilus retraction;
KW   Viral release from host cell; Virion; Virus entry into host cell.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..437
FT                   /note="Attachment protein G3P"
FT                   /id="PRO_0000003295"
FT   TRANSMEM        415..435
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          214..347
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   437 AA;  44800 MW;  E16CC41A3324B47A CRC64;
     MSIKTLISVL RVTLLTACLL PSLFFVRSAI AGPYIWEVVM YSSSGSSTPA EACEKARVVA
     DRSPDWNYTS ATPKMNGLDN SYCSVVYVSR RDPSVVNTCD DCASWKLFRK GDQCANADDT
     YNASTGICEP PPKECKEGEL FPAKGPDSPV VTSGGRNYVG DGGAPTACYQ SCEYGGNPSP
     ASCYLVKGST TTGFCNYILK GTGQSCGADS YTFSQTGDSL NPPDTPNTDP SDPNDPGCPP
     GWSWSGTTCV KTPTDPTDPT DPTTPGGDGG GDGNGGGNNN GGGNDGGTGN GDGSGGGDGN
     GAGDGSGDGD GSGTGGDGNG TCDPAKENCS TGPEGPGGEL KEPTPGTWDD AIATWEKKVE
     EAKKELKTKV KANVDQMKGA FDLNLAEGGG QLPCESMTIW GKSYSLCISD YAGQLSSLRV
     ALLLMAALIA ALILLKD