ID   G3P_BPPF3               Reviewed;         483 AA.
AC   P03624;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 79.
DE   RecName: Full=Attachment protein G3P;
DE   AltName: Full=Gene 3 protein;
DE            Short=G3P;
DE   AltName: Full=Minor coat protein;
DE   Flags: Precursor;
GN   Name=III;
OS   Pseudomonas phage Pf3 (Bacteriophage Pf3).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Tertilicivirus.
OX   NCBI_TaxID=10872;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=New York, and Nijmegen;
RX   PubMed=3928901; DOI=10.1128/jvi.56.1.268-276.1985;
RA   Luiten R.G.M., Putterman D.G., Schoenmakers J.G.G., Konings R.N.H.,
RA   Day L.A.;
RT   "Nucleotide sequence of the genome of Pf3, an IncP-1 plasmid-specific
RT   filamentous bacteriophage of Pseudomonas aeruginosa.";
RL   J. Virol. 56:268-276(1985).
RN   [2]
RP   FUNCTION.
RX   PubMed=16298408; DOI=10.1016/j.virol.2005.10.020;
RA   Holland S.J., Sanz C., Perham R.N.;
RT   "Identification and specificity of pilus adsorption proteins of filamentous
RT   bacteriophages infecting Pseudomonas aeruginosa.";
RL   Virology 345:540-548(2006).
CC   -!- FUNCTION: Plays essential roles both in the penetration of the viral
CC       genome into the bacterial host via pilus retraction and in the
CC       extrusion process. During the initial step of infection, G3P mediates
CC       adsorption of the phage to its primary receptor, the tip of host type
CC       IV PAO pilus. Subsequent interaction with the host entry receptor
CC       induces penetration of the viral DNA into the host cytoplasm. In the
CC       extrusion process, G3P mediates the release of the membrane-anchored
CC       virion from the cell via its C-terminal domain.
CC       {ECO:0000269|PubMed:16298408}.
CC   -!- SUBUNIT: Interacts with G6P; this interaction is required for proper
CC       integration of G3P and G6P into the virion. Interacts with G8P (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host membrane
CC       {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
CC       Note=Prior to assembly, G3P is found associated with the bacterial host
CC       inner membrane. There are about five copies of this protein per mature
CC       phage that are located on the head side of the filamentous virion.
CC   -!- MISCELLANEOUS: The strain Nijmegen sequence is shown.
CC   -!- SIMILARITY: Belongs to the inovirus G3P protein family. {ECO:0000305}.
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DR   EMBL; M11912; AAA88379.1; -; Genomic_DNA.
DR   EMBL; M19377; AAA88388.1; -; Genomic_DNA.
DR   PIR; A04232; VCBPI3.
DR   RefSeq; NP_040653.1; NC_001418.1.
DR   GeneID; 1260906; -.
DR   KEGG; vg:1260906; -.
DR   Proteomes; UP000001719; Genome.
DR   Proteomes; UP000009090; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0039667; P:viral entry into host cell via pilus retraction; IEA:UniProtKB-KW.
DR   GO; GO:0099045; P:viral extrusion; IEA:UniProtKB-KW.
DR   GO; GO:0039666; P:virion attachment to host cell pilus; IEA:UniProtKB-KW.
DR   InterPro; IPR008708; Neisseria_TspB.
DR   Pfam; PF05616; Neisseria_TspB; 1.
PE   3: Inferred from homology;
KW   Host membrane; Host-virus interaction; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix;
KW   Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW   Viral attachment to host cell pilus;
KW   Viral attachment to host entry receptor; Viral extrusion;
KW   Viral penetration into host cytoplasm;
KW   Viral penetration into host cytoplasm via pilus retraction;
KW   Viral release from host cell; Virion; Virus entry into host cell.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..483
FT                   /note="Attachment protein G3P"
FT                   /id="PRO_0000098208"
FT   TRANSMEM        460..480
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          324..343
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          354..378
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         419
FT                   /note="L -> F (in strain: New York)"
SQ   SEQUENCE   483 AA;  52197 MW;  DCBBAA598BA700BB CRC64;
     MGLHYLFGFC LALFSFSAIA AGPVSTEVAA GTTTYRVTNT TVRTPPNVTL SPVRDITPYV
     EKIPNKGLAQ AAQGRLIVAQ RAASVPVTGF FNVSGAVVKS GAKSFLRSAG RASGIGLGLA
     ALLEAADWVF DEEGEIVKPL PGGGSPVLMP RPVILNEYTV TGSAGQWSIS KEYEPDPRSV
     PGWYSYNGNP VWVSAVEDVG FTWRYWYFAD VLMDGQGRPN YLVAYSDSGP NEYWQDVGGY
     SLDSLPTEPE FVPLTDAELE AGIDQYYEPD PDDWRNLFPY IEPDSFTIET PIPSLDLSPV
     VSSSTNNQTG KVTVTETTTS VDFEVSDNNS SQPSISVNET TTENVYVDGD LVSSETNTTV
     TNPPSSGTST PPSSGSGSDF QLPSFCSWAT AVCDWFDWTQ EPIDEEPDLS GIISDIDDLE
     RTKDISFGSK SCPAPIALDI EFLDMSVDLS FEWFCELAGI IYFMVMASAY VLAAYITLGV
     VRG