G3P_CHAMQ
ID G3P_CHAMQ Reviewed; 16 AA.
AC C0HLQ6;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 17-JUN-2020, sequence version 1.
DT 03-AUG-2022, entry version 5.
DE RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase {ECO:0000303|PubMed:23291769};
DE Short=GAPH {ECO:0000303|PubMed:23291769};
DE EC=1.2.1.12 {ECO:0000250|UniProtKB:P09124};
DE Flags: Fragment;
OS Chattonella marina var. antiqua (Red tide flagellate) (Chattonella
OS antiqua).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Raphidophyceae; Chattonellales;
OC Chattonellaceae; Chattonella.
OX NCBI_TaxID=859642;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, AND INDUCTION.
RC STRAIN=NIES-1 {ECO:0000303|PubMed:23291769};
RX PubMed=23291769; DOI=10.1271/bbb.120543;
RA Qiu X., Shimasaki Y., Tsuyama M., Yamada T., Kuwahara R., Kawaguchi M.,
RA Honda M., Gunjikake H., Tasmin R., Shimizu M., Sato Y., Kato-Unoki Y.,
RA Nakashima T., Matsubara T., Yamasaki Y., Ichinose H., Wariishi H.,
RA Honjo T., Oshima Y.;
RT "Growth-phase dependent variation in photosynthetic activity and cellular
RT protein expression profile in the harmful raphidophyte Chattonella
RT antiqua.";
RL Biosci. Biotechnol. Biochem. 77:46-52(2013).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE, AND INDUCTION.
RC STRAIN=NIES-1 {ECO:0000303|Ref.2};
RX DOI=10.1016/j.jembe.2020.151361;
RA Qiu X., Mukai K., Shimasaki Y., Wu M., Chen C., Lu Y., Ichinose H.,
RA Nakashima T., Kato-Unoki Y., Oshima Y.;
RT "Diurnal variations in expression of photosynthesis-related proteins in the
RT harmful Raphidophyceae Chattonella marina var. antiqua.";
RL J. Exp. Mar. Biol. Ecol. 527:0-0(2020).
CC -!- FUNCTION: Catalyzes the oxidative phosphorylation of glyceraldehyde 3-
CC phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor
CC NAD. {ECO:0000250|UniProtKB:P09124}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-
CC phospho-glyceroyl phosphate + H(+) + NADH; Xref=Rhea:RHEA:10300,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57604, ChEBI:CHEBI:57945, ChEBI:CHEBI:59776; EC=1.2.1.12;
CC Evidence={ECO:0000250|UniProtKB:P09124};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 1/5. {ECO:0000250|UniProtKB:P09124}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P00362}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P09124}.
CC -!- INDUCTION: Expression shows a diurnal pattern of oscillation across the
CC 24-hour light-dark, with increased levels during the dark period (at
CC protein level) (Ref.2). Up-regulated in the early and late stationary
CC growth phases as compared to the exponential growth phase
CC (PubMed:23291769). {ECO:0000269|PubMed:23291769, ECO:0000269|Ref.2}.
CC -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase
CC family. {ECO:0000305}.
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DR AlphaFoldDB; C0HLQ6; -.
DR UniPathway; UPA00109; UER00184.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase.
FT CHAIN 1..>16
FT /note="Glyceraldehyde-3-phosphate dehydrogenase"
FT /id="PRO_0000450207"
FT BINDING 14..15
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P00362"
FT NON_TER 16
FT /evidence="ECO:0000303|PubMed:23291769"
SQ SEQUENCE 16 AA; 1576 MW; 081B10571612778E CRC64;
EGDVIATGIN GFGRIG
