ID   3NBB_COERA              Reviewed;          77 AA.
AC   P83490;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Alpha-colubritoxin {ECO:0000303|PubMed:14708577};
OS   Coelognathus radiatus (Radiated ratsnake) (Elaphe radiata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Colubridae; Colubrinae; Coelognathus.
OX   NCBI_TaxID=201391;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   PYROGLUTAMATE FORMATION AT GLN-1.
RC   TISSUE=Venom;
RX   PubMed=14708577; DOI=10.1007/s00239-003-2497-3;
RA   Fry B.G., Lumsden N.G., Wuester W., Wickramaratna J.C., Hodgson W.C.,
RA   Kini R.M.;
RT   "Isolation of a neurotoxin (alpha-colubritoxin) from a 'non-venomous'
RT   colubrid: evidence for early origin of venom in snakes.";
RL   J. Mol. Evol. 57:446-452(2003).
CC   -!- FUNCTION: Potent postsynaptic neurotoxin. Displays readily reversible
CC       competitive antagonism at the nicotinic acetylcholine receptor (nAChR).
CC       {ECO:0000269|PubMed:14708577}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14708577}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=8498; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:14708577};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Boigatoxin sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P83490; -.
DR   SMR; P83490; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Pyrrolidone carboxylic acid; Secreted; Toxin.
FT   CHAIN           1..77
FT                   /note="Alpha-colubritoxin"
FT                   /evidence="ECO:0000269|PubMed:14708577"
FT                   /id="PRO_0000093668"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:14708577"
FT   DISULFID        10..34
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        13..21
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        27..55
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        59..70
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        71..76
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
SQ   SEQUENCE   77 AA;  8526 MW;  D08E2E5906321B41 CRC64;
     QAIGPPYGLC FQCNQKTSSD CTEARRCSPF HEKCYTLYQP DENWMKSSGL SHFGCGKQCP
     TAGPEGRVTC CLTPRCN