ID   G3ST2_MOUSE             Reviewed;         394 AA.
AC   Q6XQH0;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Galactose-3-O-sulfotransferase 2;
DE            Short=Gal3ST-2;
DE            EC=2.8.2.-;
DE   AltName: Full=Beta-galactose-3-O-sulfotransferase 2;
DE   AltName: Full=Gal-beta-1, 3-GalNAc 3'-sulfotransferase 2;
GN   Name=Gal3st2; Synonyms=Gm988;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=CD-1; TISSUE=Colon;
RA   Seko A., Yamashita K.;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers a sulfate group to the hydroxyl group at C3 of non-
CC       reducing beta-galactosyl residues. Acts both on type 1 (Gal-beta-1,3-
CC       GlcNAc) and type 2 (Gal-beta-1,4-GlcNAc) chains with similar efficiency
CC       (By similarity). {ECO:0000250}.
CC   -!- ACTIVITY REGULATION: Strongly inhibited by Cu(2+) and Zn(2+).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC       {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AY216522; AAP51032.1; -; mRNA.
DR   CCDS; CCDS56641.1; -.
DR   RefSeq; NP_001192178.1; NM_001205249.1.
DR   AlphaFoldDB; Q6XQH0; -.
DR   STRING; 10090.ENSMUSP00000136012; -.
DR   GlyGen; Q6XQH0; 4 sites.
DR   PhosphoSitePlus; Q6XQH0; -.
DR   MaxQB; Q6XQH0; -.
DR   PaxDb; Q6XQH0; -.
DR   PRIDE; Q6XQH0; -.
DR   Ensembl; ENSMUST00000178757; ENSMUSP00000136012; ENSMUSG00000093805.
DR   GeneID; 100041596; -.
DR   KEGG; mmu:100041596; -.
DR   UCSC; uc029qsc.1; mouse.
DR   CTD; 100041596; -.
DR   MGI; MGI:2685834; Gal3st2.
DR   VEuPathDB; HostDB:ENSMUSG00000093805; -.
DR   eggNOG; ENOG502QSHR; Eukaryota.
DR   GeneTree; ENSGT00950000182923; -.
DR   HOGENOM; CLU_040616_1_1_1; -.
DR   InParanoid; Q6XQH0; -.
DR   OMA; IHLGYPW; -.
DR   PhylomeDB; Q6XQH0; -.
DR   UniPathway; UPA00353; -.
DR   BioGRID-ORCS; 100041596; 1 hit in 61 CRISPR screens.
DR   ChiTaRS; Gal3st2b; mouse.
DR   PRO; PR:Q6XQH0; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; Q6XQH0; protein.
DR   Bgee; ENSMUSG00000093805; Expressed in colon and 19 other tissues.
DR   ExpressionAtlas; Q6XQH0; baseline and differential.
DR   Genevisible; Q6XQH0; MM.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IDA:MGI.
DR   GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IDA:MGI.
DR   GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR   GO; GO:0008146; F:sulfotransferase activity; ISO:MGI.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0009101; P:glycoprotein biosynthetic process; IDA:MGI.
DR   GO; GO:0051923; P:sulfation; IDA:MGI.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR14647; PTHR14647; 1.
DR   Pfam; PF06990; Gal-3-0_sulfotr; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Signal-anchor;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..394
FT                   /note="Galactose-3-O-sulfotransferase 2"
FT                   /id="PRO_0000085204"
FT   TOPO_DOM        1..8
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..394
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        72
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        284
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        326
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   394 AA;  46909 MW;  20DB234D8A5ED6A9 CRC64;
     MWGSQHRSFQ VALWFLVLAV FLLVGFLHVD FRLLIPDKVQ EPPVTNIMFL KTHKTASSTI
     LNILYRFSES HNLSTALPEG SRVHLGYPWF FVTRYVEGLK QDAHLQHHFN IMCNHLRFNY
     PEVQKVMPRD TFYFSILRNP VFQLESSFIY YKDYAPAFQR AKSLDEFLAD PWKYYNASVS
     LKNVYAKNNM WFDFGFDNNA PADMDYVRKR LAEVEQRFHL VLIADYFDES MVLLRRRLRW
     QLDDVVSFKL NVRSQSTVSH LTPESQERVQ HWCALDWQLY QHFNRTFWTQ LHAELSPRQL
     TEEVEQLRER QRELMALCLQ DPEPKNLTHI DDQNLRPYQG GKANILGYNL RHGLDTTTLH
     ICQRMAMPEL QHMAHMYSLQ FPDKTPKDIP FLKK