ID   G3ST3_BOVIN             Reviewed;         427 AA.
AC   Q0VCH4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Galactose-3-O-sulfotransferase 3;
DE            Short=Gal3ST-3;
DE            EC=2.8.2.-;
DE   AltName: Full=Beta-galactose-3-O-sulfotransferase 3;
DE   AltName: Full=Gal-beta-1, 3-GalNAc 3'-sulfotransferase 3;
GN   Name=GAL3ST3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal pons;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers a sulfate to position 3 of non-reducing beta-
CC       galactosyl residues in N-glycans and core2-branched O-glycans. Has high
CC       activity towards Gal-beta-1,4-GlcNAc, Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc
CC       and lower activity towards Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC       {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; BC120166; AAI20167.1; -; mRNA.
DR   RefSeq; NP_001069308.1; NM_001075840.1.
DR   RefSeq; XP_005227163.1; XM_005227106.2.
DR   AlphaFoldDB; Q0VCH4; -.
DR   STRING; 9913.ENSBTAP00000003590; -.
DR   PaxDb; Q0VCH4; -.
DR   PRIDE; Q0VCH4; -.
DR   Ensembl; ENSBTAT00000003590; ENSBTAP00000003590; ENSBTAG00000002772.
DR   GeneID; 523401; -.
DR   KEGG; bta:523401; -.
DR   CTD; 89792; -.
DR   VEuPathDB; HostDB:ENSBTAG00000002772; -.
DR   VGNC; VGNC:29215; GAL3ST3.
DR   eggNOG; ENOG502QPNT; Eukaryota.
DR   GeneTree; ENSGT00950000182923; -.
DR   HOGENOM; CLU_040616_1_0_1; -.
DR   InParanoid; Q0VCH4; -.
DR   OMA; PDEKDSM; -.
DR   OrthoDB; 1385827at2759; -.
DR   TreeFam; TF314802; -.
DR   UniPathway; UPA00353; -.
DR   Proteomes; UP000009136; Chromosome 29.
DR   Bgee; ENSBTAG00000002772; Expressed in retina and 66 other tissues.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR   GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR14647; PTHR14647; 1.
DR   Pfam; PF06990; Gal-3-0_sulfotr; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Golgi apparatus; Magnesium; Membrane; Reference proteome;
KW   Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..427
FT                   /note="Galactose-3-O-sulfotransferase 3"
FT                   /id="PRO_0000315389"
FT   TOPO_DOM        1..19
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        20..40
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        41..427
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   REGION          404..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        410..427
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        90
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        301
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   427 AA;  48699 MW;  F1204632C9F6ED42 CRC64;
     MPPIFQRLQQ ATKMSRRKIL LLVLGCSTLS LLIHQGAQLS WYPKLFPLSC PPLQDSPPRP
     KHMTVAFLKT HKTAGTTVQN ILFRFAERHN LTVALPHPSC EHQFCYPRNF SAHFVHPATR
     PPHVLASHLR FDRAELQRLM PPGTVYVTIL REPAAMFESL FSYYNQYCPA FRRVPNASLE
     AFLRAPEAYY RAGEHFAMFA HNTLAYDLGG DNERSPRDDD AYLAGLIRQV EEVFSLVMIA
     EYFDESLVLL RRLLAWDLDD VLYARLNARA ASSRLAAIPA ALAQAARAWN ALDAGLYDHF
     NATFWRRVAS AGRACVEREA RELREARERL LRRCFGDEPV LRPAAQIRTK QLQPWQPSRK
     VDIMGYDLPS GRAGPATEAC LKLAMPEVQY SSYLLRKQKR RGGMRLRPEP VLDNPPPRPI
     RALRPGH