G3ST3_HUMAN
ID G3ST3_HUMAN Reviewed; 431 AA.
AC Q96A11; Q14D05;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Galactose-3-O-sulfotransferase 3;
DE Short=Gal3ST-3;
DE EC=2.8.2.-;
DE AltName: Full=Beta-galactose-3-O-sulfotransferase 3;
DE Short=Gal3ST3;
DE AltName: Full=Gal-beta-1, 3-GalNAc 3'-sulfotransferase 3;
GN Name=GAL3ST3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=11356829; DOI=10.1074/jbc.m100348200;
RA El-Fasakhany F.M., Uchimura K., Kannagi R., Muramatsu T.;
RT "A novel human Gal-3-O-sulfotransferase: molecular cloning,
RT characterization, and its implications in biosynthesis of (SO4-3)Galbeta 1-
RT 4(Fucalpha 1-3)GlcNAc.";
RL J. Biol. Chem. 276:26988-26994(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11323440; DOI=10.1074/jbc.m103135200;
RA Suzuki A., Hiraoka N., Suzuki M., Angata K., Misra A.K., McAuliffe J.,
RA Hindsgaul O., Fukuda M.;
RT "Molecular cloning and expression of a novel human beta-Gal-3-O-
RT sulfotransferase that acts preferentially on N-acetyllactosamine in N- and
RT O-glycans.";
RL J. Biol. Chem. 276:24388-24395(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transfers a sulfate to position 3 of non-reducing beta-
CC galactosyl residues in N-glycans and core2-branched O-glycans. Has high
CC activity towards Gal-beta-1,4-GlcNAc, Gal-beta-1,4(Fuc-alpha-1,3)GlcNAc
CC and lower activity towards Gal-beta-1,3(Fuc-alpha-1,4)GlcNAc.
CC {ECO:0000269|PubMed:11323440, ECO:0000269|PubMed:11356829}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.0-8.0.;
CC -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC -!- INTERACTION:
CC Q96A11; Q6NTF9-3: RHBDD2; NbExp=3; IntAct=EBI-17590461, EBI-17589229;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in thyroid, brain, kidney, heart
CC and spinal cord. {ECO:0000269|PubMed:11323440,
CC ECO:0000269|PubMed:11356829}.
CC -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC {ECO:0000305}.
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DR EMBL; AB053232; BAB61900.1; -; mRNA.
DR EMBL; AY026481; AAK01945.1; -; mRNA.
DR EMBL; BC113552; AAI13553.1; -; mRNA.
DR EMBL; BC113554; AAI13555.1; -; mRNA.
DR CCDS; CCDS8128.1; -.
DR RefSeq; NP_149025.1; NM_033036.2.
DR AlphaFoldDB; Q96A11; -.
DR BioGRID; 124603; 1.
DR IntAct; Q96A11; 1.
DR STRING; 9606.ENSP00000308591; -.
DR GlyGen; Q96A11; 4 sites.
DR iPTMnet; Q96A11; -.
DR PhosphoSitePlus; Q96A11; -.
DR BioMuta; GAL3ST3; -.
DR DMDM; 47116425; -.
DR EPD; Q96A11; -.
DR MassIVE; Q96A11; -.
DR PaxDb; Q96A11; -.
DR PeptideAtlas; Q96A11; -.
DR PRIDE; Q96A11; -.
DR ProteomicsDB; 75891; -.
DR Antibodypedia; 2654; 45 antibodies from 15 providers.
DR DNASU; 89792; -.
DR Ensembl; ENST00000312006.5; ENSP00000308591.3; ENSG00000175229.7.
DR Ensembl; ENST00000527878.1; ENSP00000434829.1; ENSG00000175229.7.
DR GeneID; 89792; -.
DR KEGG; hsa:89792; -.
DR MANE-Select; ENST00000312006.5; ENSP00000308591.3; NM_033036.3; NP_149025.1.
DR UCSC; uc001ogv.4; human.
DR CTD; 89792; -.
DR DisGeNET; 89792; -.
DR GeneCards; GAL3ST3; -.
DR HGNC; HGNC:24144; GAL3ST3.
DR HPA; ENSG00000175229; Tissue enhanced (thyroid).
DR MIM; 608234; gene.
DR neXtProt; NX_Q96A11; -.
DR OpenTargets; ENSG00000175229; -.
DR PharmGKB; PA134864180; -.
DR VEuPathDB; HostDB:ENSG00000175229; -.
DR eggNOG; ENOG502QPNT; Eukaryota.
DR GeneTree; ENSGT00950000182923; -.
DR HOGENOM; CLU_040616_1_0_1; -.
DR InParanoid; Q96A11; -.
DR OMA; PDEKDSM; -.
DR OrthoDB; 1385827at2759; -.
DR PhylomeDB; Q96A11; -.
DR TreeFam; TF314802; -.
DR PathwayCommons; Q96A11; -.
DR SignaLink; Q96A11; -.
DR UniPathway; UPA00353; -.
DR BioGRID-ORCS; 89792; 12 hits in 1063 CRISPR screens.
DR GeneWiki; GAL3ST3; -.
DR GenomeRNAi; 89792; -.
DR Pharos; Q96A11; Tdark.
DR PRO; PR:Q96A11; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96A11; protein.
DR Bgee; ENSG00000175229; Expressed in left lobe of thyroid gland and 62 other tissues.
DR Genevisible; Q96A11; HS.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0050656; F:3'-phosphoadenosine 5'-phosphosulfate binding; NAS:UniProtKB.
DR GO; GO:0030246; F:carbohydrate binding; NAS:UniProtKB.
DR GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IDA:UniProtKB.
DR GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR GO; GO:0050698; F:proteoglycan sulfotransferase activity; NAS:UniProtKB.
DR GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR GO; GO:0005996; P:monosaccharide metabolic process; NAS:UniProtKB.
DR GO; GO:0009311; P:oligosaccharide metabolic process; NAS:UniProtKB.
DR GO; GO:0030309; P:poly-N-acetyllactosamine metabolic process; NAS:UniProtKB.
DR GO; GO:0030166; P:proteoglycan biosynthetic process; NAS:UniProtKB.
DR GO; GO:0006790; P:sulfur compound metabolic process; NAS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR14647; PTHR14647; 1.
DR Pfam; PF06990; Gal-3-0_sulfotr; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Magnesium; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..431
FT /note="Galactose-3-O-sulfotransferase 3"
FT /id="PRO_0000085206"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..431
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 399..431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 411..431
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 177
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 221
FT /note="A -> D (in dbSNP:rs35285455)"
FT /id="VAR_053988"
FT VARIANT 410
FT /note="E -> A (in dbSNP:rs4565902)"
FT /id="VAR_053989"
SQ SEQUENCE 431 AA; 48956 MW; 3E1B8418A72A5EB6 CRC64;
MPPILQRLQQ ATKMMSRRKI LLLVLGCSTV SLLIHQGAQL SWYPKLFPLS CPPLRNSPPR
PKHMTVAFLK THKTAGTTVQ NILFRFAERH NLTVALPHPS CEHQFCYPRN FSAHFVHPAT
RPPHVLASHL RFDRAELERL MPPSTVYVTI LREPAAMFES LFSYYNQYCP AFRRVPNASL
EAFLRAPEAY YRAGEHFAMF AHNTLAYDLG GDNERSPRDD AAYLAGLIRQ VEEVFSLVMI
AEYFDESLVL LRRLLAWDLD DVLYAKLNAR AASSRLAAIP AALARAARTW NALDAGLYDH
FNATFWRHVA RAGRACVERE ARELREARQR LLRRCFGDEP LLRPAAQIRT KQLQPWQPSR
KVDIMGYDLP GGGAGPATEA CLKLAMPEVQ YSNYLLRKQK RRGGARARPE PVLDNPPPRP
IRVLPRGPQG P
